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Articles by Pavan Kumar
Total Records ( 3 ) for Pavan Kumar
  Sunil Kumar , Z.F. Bhat and Pavan Kumar
  A study was conducted to evaluate the effect of apple pulp on the quality characteristics of Shrikhand. Various levels of apple pulp viz. 0, 10, 20 and 30 percent were used in the preparation of Shrikhand replacing chakka in the formulation. Further the use of dried ‘Celosia argentea’ (flower part only) as a colouring agent in the preparation of the Shrikhand containing optimum level of the apple pulp was explored. The protein, fat and ash percent of the Shrikhand decreased significantly (p<0.05) whereas moisture content increased significantly (p<0.05) with increasing level of apple pulp. On the basis of various sensory parameters, Shrikhand containing 20% apple pulp and dried Celosia argentea flower was selected as optimum. The product was further packed in polystyrene cups and stored under refrigerated conditions at 4±1°C for a period of three weeks. The mean scores of all the sensory parameters decreased significantly (p<0.05) with storage. Total plate count and psychrophilic count increased significantly (p<0.05) whereas coliforms were not detected throughout the period of storage. Thiobarbituric acid reacting substances (TBARS) values also increased significantly (p<0.05) with storage period. The product was acceptable for a period of three weeks under refrigerated conditions.
  Pramod Kumar Singh , Sunil Kumar , Pavan Kumar and Z.F. Bhat
  Growing awareness and preference of the consumers towards the minimum processed meat with natural sensory and nutritive characteristics leads to the introduction of non thermal techniques for the decontamination of meat. The Pulse Light (PL) and the Pulse Electric Field (PEF) are the latest non thermal techniques employed for decontamination of meat without production of any harmful toxic compounds. These techniques retain the natural freshness, colour and nutritive characteristics of meat in comparison to thermal and heat processing. The present review discusses the PL and PEF techniques in enhancing the storage life of meat.
  Veena S. Anil , Premraj Rajkumar , Pavan Kumar and M. K. Mathew
  Stress responses in both plants and yeast utilize calcium-mediated signaling. A yeast strain, K616, which lacks Ca2+ pumps, requires micromolar Ca2+ for growth. In medium containing 100 µM Ca2+, K616 can withstand osmotic stress (750 mM sorbitol) and ionic stress (300 mM KCl) but not hypersodic stress (300 mM NaCl). Heterologous expression of the endoplasmic reticulum-located Arabidopsis thaliana Ca2+-ATPase, ACA2, permits K616 to grow under NaCl stress even in Ca2+-depleted medium. All stresses tested generated transient elevation of cytosolic Ca2+ in wild type yeast, K601, whereas NaCl alone induced prolonged elevation of cytosolic Ca2+ in K616. Both the Ca2+ transient and survival of cultures subjected to NaCl stress was similar for the ACA2 transformant and K601. However, whereas K601 maintained low cytosolic Na+ predominantly by pumping it out across the plasma membrane, the transformant sequestered Na+ in internal organelles. This sequestration requires the presence of an endomembrane Na+/H+-antiporter, NHX1, which does not play a significant role in salt tolerance of wild type yeast except at acidic pH. Transcript levels of the plasma membrane Na+-ATPase, ENA1, were strongly induced only in K601, whereas NHX1 was strongly induced in both K601 and the ACA2 transformant. The calmodulin kinase inhibitor KN62 significantly reduced the salt tolerance of the ACA2 transformant and the transcriptional induction of NHX1. Thus, the heterologous expression of a plant endomembrane Ca2+ pump results in the rapid depletion of cytosolic Ca2+ and the activation of an alternate mechanism for surviving saline stress.
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