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Articles by Paul Klenerman
Total Records ( 3 ) for Paul Klenerman
  Victoria Kasprowicz , Julian Schulze zur Wiesch , Thomas Kuntzen , Brian E. Nolan , Steven Longworth , Andrew Berical , Jenna Blum , Cory McMahon , Laura L. Reyor , Nahel Elias , William W. Kwok , Barbara G. McGovern , Gordon Freeman , Raymond T. Chung , Paul Klenerman , Lia Lewis-Ximenez , Bruce D. Walker , Todd M. Allen , Arthur Y. Kim and Georg M. Lauer
  We monitored expression of PD-1 (a mediator of T-cell exhaustion and viral persistence) on hepatitis C virus (HCV)-specific CD8+ and CD4+ T cells from blood and liver during acute and chronic infections and after the resolved infection stage. PD-1 expression on HCV-specific T cells was high early in acute infection irrespective of clinical outcome, and most cells continued to express PD-1 in resolved and chronic stages of infection; intrahepatic expression levels were especially high. Our results suggest that an analysis of PD-1 expression alone is not sufficient to predict infection outcome or to determine T-cell functionality in HCV infection.
  Christoph Neumann-Haefelin , David N. Frick , Jing Jing Wang , Oliver G. Pybus , Shadi Salloum , Gagandeep S. Narula , Anna Eckart , Andrea Biezynski , Thomas Eiermann , Paul Klenerman , Sergei Viazov , Michael Roggendorf , Robert Thimme , Markus Reiser and Jorg Timm
  Failure of the adaptive immune response to control infection with the hepatitis C virus (HCV) can result from mutational escape in targeted T-cell epitopes. Recent studies suggest that T-cell immune pressure is an important factor in the evolution of the nonstructural proteins in HCV. The aim of this study was to characterize the forces that contribute to viral evolution in an HLA-A*01-restricted epitope in HCV NS3. This epitope represents a potentially attractive target for vaccination strategies since it is conserved across all genotypes. In our cohort of subjects with chronic HCV infection (genotype 1b or 3a), it is a frequently recognized CD8 epitope in HLA-A*01-positive subjects. Viral sequence data reveal that an escape variant is the dominant residue in both genotypes. The predominant Y1444F substitution seemingly impairs binding to the HLA-A*01 molecule, which may have an important impact on the ability to prime a functional CD8 response upon infection. Interestingly, a case of evolution toward the prototype sequence was observed during chronic infection, possibly because the helicase activity of the protein containing the Y1444F substitution is reduced compared to the prototype sequence. Comparison of HCV sequences from Asia and Europe suggests that the frequency of the HLA-A*01 allele in a population may influence the frequency of the escape variant in circulating strains. These data suggest a complex interaction of multiple forces shaping the evolution of HCV in which immune pressure both within the individual and also at the population level in addition to functional constraints are important contributing factors.
  Shadi Salloum , Cesar Oniangue-Ndza , Christoph Neumann-Haefelin , Laura Hudson , Silvia Giugliano , Marc aus dem Siepen , Jacob Nattermann , Ulrich Spengler , Georg M. Lauer , Manfred Wiese , Paul Klenerman , Helen Bright , Norbert Scherbaum , Robert Thimme , Michael Roggendorf , Sergei Viazov and Joerg Timm
  The inherent sequence diversity of the hepatitis C virus (HCV) represents a major hurdle for the adaptive immune system to control viral replication. Mutational escape within targeted CD8 epitopes during acute HCV infection has been well documented and is one possible mechanism for T-cell failure. HLA-B*08 was recently identified as one HLA class I allele associated with spontaneous clearance of HCV replication. Selection of escape mutations in the immunodominant HLA-B*08-restricted epitope HSKKKCDEL1395-1403 was observed during acute infection. However, little is known about the impact of escape mutations in this epitope on viral replication capacity. Their previously reported reversion back toward the consensus residue in patients who do not possess the B*08 allele suggests that the consensus sequence in this epitope is advantageous for viral replication in the absence of immune pressure. The aim of this study was to determine the impact of mutational escape from this immunodominant epitope on viral replication. We analyzed it with a patient cohort with chronic HCV genotype 1b infection and in a single-source outbreak (genotype 1b). Sequence changes in this highly conserved region are rare and selected almost exclusively in the presence of the HLA-B*08 allele. When tested in the subgenomic replicon (Con1), the observed mutations reduce viral replication compared with the prototype sequence. The results provide direct evidence that escape mutations in this epitope are associated with fitness costs and that the antiviral effect of HLA-B*08-restricted T cells is sufficiently strong to force the virus to adopt a relatively unfavorable sequence.
 
 
 
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