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Articles by Parvati Sharma
Total Records ( 3 ) for Parvati Sharma
  Ram C. Sihag and Parvati Sharma
  Diseases are considered to be the major constraint in aquaculture production. They cause mortality in shrimp larviculture and fish hatcheries. They are also a constraint on consistent production of fish and shell fish. Traditionally, the control of diseases in aquaculture has relied on the use of chemical compounds. More recently probiotic microorganisms and vaccination or other forms of immunostimulation have also been employed. The abuse of antimicrobials can result in the development of resistant strains of bacteria. Such resistance can be readily transferred to other strains, either following alterations to the existing genome or by transfer of genetic material between cells through plasmids or bacteriophages. The massive use of antibiotics for the control of diseases has been questioned by acquisition of antibiotic resistance in disease causing agents and the need of alternative measures to control these diseases is of prime importance. In recent years, probiotics have a center stage and are used as alternative measures to control the fish diseases. Probiotics have been used by man for millennia since the time humans first consumed fermented milk products. Probiotics can be essential for the normal digestive, endocrine and immunological functions of the bowel. They inhibit pathogenic microorganisms and have been used therapeutically to treat a variety of gastrointestinal and even systemic disorders. Probiotics transiently colonize the bowel and except when used to treat an acute disorder, must be regularly consumed to maintain benefit. Use of microbial probiotics to promote health maintenance and disease prevention and control is now widely accepted as the new ecofriendly alternative measures for sustainable aquaculture.
  Parvati Sharma , Ram C. Sihag and S.K. Gahlawat
  The Epizootic Ulcerative Syndrome (EUS) is a dreaded disease of mrigal (Cirrhinus mrigala Ham.) inflicting heavy mortality in the latter fish at the farmers’ ponds. To realize a good harvest, the control of this disease through the use of some probiotics is most important. The aim of study was to ascertain the effect of two probiotics on various blood and life parameters of mrigal (C. mrigala). Two commercially available probiotics with different compositions were tested for this purpose. In vitro trials were performed by the usual ‘well poisoning method’ whereas in vivo trials were performed in the fishes that were subjected to induced pathogenicity. In the treated fishes, periodic observations were recorded on their different hematological (viz., amount of hemoglobin, total erythrocyte count and total leukocyte count), survival and growth (length and weight) parameters. In the in vitro trials both probiotics developed clear zones of inhibition. However, the zone shown by probiotic-1 (having bacteria only) was smaller in size than that by probiotic-2 (in addition to bacteria also having vitamins, enzymes and salts), revealing the probable greater efficacy of the latter than the former. In the in vivo trials, the values of all the hematological parameters were found to decrease (leukocyte count increased) in the fish having induced pathogenicity and so were survival and growth parameters. But these values showed significant increase (leukocyte count decreased) in the probiotic treated fishes. This confirmed the useful role of both the probiotics in controlling the EUS disease in the mrigal. However, probiotic-2 was found to be more effective than probiotic-1 in increasing the hematological parameters and growth and survival in this fish.
  Parvati Sharma and R.C. Sihag
  The study was conducted for 8 weeks to determine pathogenic effect of different fish pathogens in Cirrihinus mrigal (C. mrigala) which was infected with EUS disease. The pathogenic organisms (bacteria and fungi) collected from the infected part of the disease fish. Isolation and screening of microbes were carried out with the help of a number of biochemical tests. A total of eight bacterial isolates were obtained from the diseased fish. Out of eight, six bacteria viz., Streptococcus grp Q1, Aeromonas hydrophilla, Shigella spp., Streptococcus faecalis, Cellobiosococcus sciuri, Micrococcus luteus were found to be pathogenic. The fungus, Aphanomyces invadens was also isolated from the diseased fishes. The pathogenicity of disease causing organisms was tested through in vitro and in vivo experiments in different treatments. The result of experiment was found to be significant at level of p≤0.05.
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