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Articles by Pankaj Kumar Bhowmik
Total Records ( 7 ) for Pankaj Kumar Bhowmik
  Pankaj Kumar Bhowmik , Toshiyuki Matsui and A.K.M. Shameem Alam
  A 1-aminocyclopropane-1-carboxylate (ACC) synthase cDNA from Asparagus spears was isolated and sequenced as a continuation after cloning, sequencing and expression analysis of ACC oxidase. The partial cDNA clone encoded an mRNA of 968 bp and the derived amino acid sequence was highly homologous to ACC synthase from bamboo banana, rice and wheat. Northern blot analysis showed increase level of pAS-ACS mRNA until 8 h at 20°C, which coincided with ethylene production and the ACC synthase activity, suggesting that the increase might be a response to the wounding associated with harvest.
  Pankaj Kumar Bhowmik , Toshiyuki Matsui , Masayuki Fujita , Md. Zakir Hossain and Eizou Miki
  Two inhibitors of the first enzyme phenylalanine ammonia lyase (PAL) in the phenylpropanoid pathway were used to investigate the role of phenolic metabolism in lignification and toughness of Asparagus spears. Spears were soaked for 1 h at 20°C in aqueous solution of α-aminooxi-β-phenylpropionic acid (AOPP) at 0, 100, 300 and 600 μM. Liquid 2,5-Norbornadiene (NBD) was applied with a micropipette onto a sheet of filter paper in a 6.4 liter big jar which was immediately sealed with a cover. Liquid NBD readily volatilized at 25°C in a closed chamber. The amount of liquid applied was calculated to give the desired gaseous concentration (6000 μl L-1) in the jar. Both AOPP and NBD inhibited PAL activity and significantly decreased fiber content in both top and bottom portions of the spears. Northern blot analysis showed that expression in mRNA level coincided well with the changes in PAL activity and fiber content. These results confirm the view that AOPP and NBD, inhibitors of PAL enzyme activity, reduce PAL gene expression and decrease the fiber content of Asparagus spears, suggesting that downstream products of PAL enzyme activity may have a regulatory role in the lignification process.
  Toshiyuki Matsui , Pankaj Kumar Bhowmik and Kyosuke Yokozeki
  Previously isolated cDNA clones encoding glutamine synthetase (GS, EC 6.3.1.2) genes from asparagus and broccoli were observed that the level of transcripts decreased in both plant species during storage. In order to identify factors regulating the postharvest deterioration of another important Asian vegetable bamboo (Phyllostachys edulis) shoot, the present study was conducted to isolate glutamine synthetase (pBA-GS) cDNA clone from a cDNA library prepared from harvested bamboo shoot using reverse transcriptase-polymerase chain reaction. The partial cDNA clone encodes an mRNA of 832 bp and the derived amino acid sequence is highly homologous to GS from rice, barely and maize. Northern blot analysis showed decrease level of pBA-GS mRNA until 9 d at 20°C, which coincided well with enzyme activity and ammonia accumulation. Results demonstrated that GS activity decrease during the postharvest storage of bamboo shoot which is a highly perishable vegetable like asparagus spears and broccoli head and is likely to be a critical feature of their postharvest physiology.
  Toshiyuki Matsui , Pankaj Kumar Bhowmik and Kyosuke Yokozeki
  In plants, phenylalanine ammonia-lyase (PAL; EC 4.3.1.5) catalyzes the conversion of L-phenylalanine to trans cinnamic acid in the first step of phenylpropanoid pathway. We previously isolated cDNA clones encoding PAL from asparagus spears and observed that the level of transcripts correlated well with increase enzyme activity and lignification. In order to identify factors regulating the postharvest deterioration of another important Asian vegetable bamboo (Phyllostachys edulis) shoot, we have now isolated PAL (pBA-PAL), cDNA clone from a cDNA library prepared from harvested bamboo shoot using reverse transcriptase-polymerase chain reaction. The partial cDNA clone encodes an mRNA of 527 bp and the derived amino acid sequence is highly homologous to PAL from rice, barely, maize and asparagus. Northern blot analysis showed increasing trend in the level of pBA-PAL mRNA reaching a peak after 3 d of storage at 20°C, which coincided well with enzyme activity. Our results demonstrate that during the postharvest storage of bamboo shoot PAL activity is regulated by increase level of pBA-PAL mRNA suggesting that the increase might be a response to the wounding associated with harvest.
  Toshiyukii Matsui , Pankaj Kumar Bhowmik and Kyousuke Yokozeki
  Broccoli (Brassica oleracea L. cv. Hartland) cDNA clone coding the enzyme glutamine synthetase (GS; EC 6.3.1.2.) was isolated from a cDNA library prepared from broccoli head using reverse transcription-polymerase chain reaction (RT-PCR). The partial cDNA clone encodes an mRNA of 695 bp and the derived amino acid sequence is highly homologus to GS from rice cytosol, maize and European grape. GS has a central role in plant nitrogen metabolism and a key enzyme in the assimilation of ammonia. Broccoli head quality deteriorates rapidly after harvest and is associated with an increase in the ammonia content of the floret portions. In order to define the factors contributing to postharvest deterioration of broccoli head, the changes in GS activity and gene expression in the floret and branchlet portions of the broccoli were examined during storage. Northern blot analysis showed that the level of transcript of GS fluctuated in the floret and branchlet portions during storage. Expression of GS gene in the floret portion was observed more clearly but it was weak in the branchlet portion that might be due to the lower activity of GS in branchlet portions.
  Toshiyuki Matsui , Pankaj Kumar Bhowmik and Kyosuke Yokozeki
  Actin is one of the most ubiquitous and conserved eukaryotic protein. All eukaryotic cells, from yeast to plants to animals, have an internal framework called the cytoskeleton. The plant actin cytoskeleton is central to many different sub-cellular processes and actin genes have been the focus of numerous scientific studies because of their involvement in basic cellular processes. In order to explain the structure and evolution of monocot actin gene family we have cloned and sequenced a 515 bp cDNA sequence from moso bamboo (Phyllostachys edulis) shoot. Phylogenetic analysis of the bamboo actin along with asparagus actin and those previously published in the database, indicates that monocots actin genes are highly conserved and actin multigene family underwent many gene duplication events early in the evolution of angiosperms.
  Pankaj Kumar Bhowmik , Toshyuki Matsui and Zakaria Ahmed
  Changes in the activities of acid invertase and sucrose synthase during germination of wheat (Triticum aestivum L. cv. Kanchan) seeds were investigated. The activities of both acid invertase and sucrose synthase were changed during growth of seedlings. Until third day of germination their activities increased and thereafter decreased. The activity of acid invertase in the soluble fraction was higher than that of cell wall- bound fraction and for soluble fraction coleoptiles showed a significantly higher acid invertase activity than roots. But in the cell wall-bound fraction a higher activity was found in roots. Sucrose synthase activity was also higher in roots than in coleoptiles. Sucrose content was negatively correlated with the acid invertase activity in both coleoptiles and roots accounting well for the relation between substrate and the activity. Fructose content was significantly higher than the glucose and sucrose content and the amount of sugar was higher in roots than in coleoptiles.
 
 
 
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