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Articles by P.V. Bramhachari
Total Records ( 4 ) for P.V. Bramhachari
  Siddhartha Eadlapalli , Sarojamma Vemula , P.V. Bramhachari and Ramakrishna Vadde
  Background: Antibiotic resistance become global concern due to over use and getting drug resistance in bacteria drawn attention for best candidate from natural resources like spices which were using since, ancient days in culinary and also in traditional medicine like Ayurveda for development of new antimicrobial compounds. Methodology: Methanolic extracts of 20 routinely consumed spices in Indian culinary were evaluated for their total phenolics, flavonoids, terpenoids and alkaloids with antioxidant and antibacterial potential against two Gram-positive bacteria (Staphylococcus aureus and Bacillus subtilis) and two Gram-negative bacteria (Escherichia coli and Klebsiella pneumonia). Results: Results revealed that higher levels of total phenolics were noticed in clove (175±21 mg GAE g–1) followed by star anise, bay leaf and caper. Terpenoid rich caper (639±19 mg LE g–1) exhibited elevated levels of antioxidant potential (642±6 mg TE g–1) as compared to other spices. Turmeric exhibited highest amount of flavonoids (31.4±2.34 mg RE g–1) followed by clove and black pepper. Mustard showed higher alkaloid content (1.6±0.08 mg AE g–1) followed by chilli and black pepper. The Gram-positive and Gram-negative bacteria were exhibited an increased growth inhibition (antibacterial and bactericidal activities) at lower concentration of garlic (2.5 μg GAE disc–1), cinnamon (4.5 μg GAE disc–1) and tamarind extracts (6 μg GAE disc–1) as compared to the standard antibiotic and streptomycin (20 μg disc–1). Conclusion: The results concluded that the phenolics and terpenoid rich spices exhibited elevated antioxidant, antibacterial and bactericidal activities. Indian spices could also be used as potential antimicrobials that develop the promising leads to the pharmaceutical industry.
  K.V. Deepika , B. Anand Kumar , S. Gnanender and P.V. Bramhachari
  Biosurfactant-producing bacteria, isolate KVD1, was isolated from Krishna river delta mangrove sediments. The taxonomic identification of KVD1 strain was done by sequencing of 16S rRNA gene showed that it formed a coherent cluster with the clad that comprised of Pseudomonas aeruginosa to be its closest phylogenetic neighbour. Strikingly, for the first time Pseudomonas aeruginosa strain KVD1 isolated from mangroves was shown to produce highest biosurfactant (4.08 g L-1) in optimised MSM containing 1.5% diesel as sole carbon source, 1% (NH4)2SO4 as nitrogen source and 1 g L-1 NaCl, at pH (7±2), 30°C, 150 rpm. The biosurfactant was obtained by cold acetone extraction method showed emulsification activity of E24% = 61±2% with n-hexadecane. The stability of the biosurfactant at different salinity, pH and temperature was also investigated. This biosurfactant also exhibited significant efficiency in oil recovery sand experiment. The present study emphasizes that biosurfactant produced by strain KVD1 is critical to open new biotechnological applications in enhanced oil removal from spillages and also as cleaning and emulsifying agents in the industry.
  P.V. Bramhachari , J. Ravichand , K.V. Deepika , P. Yalamanda and K.V. Chaitanya
  The aim of the present study was to isolate and characterize the bacterial strain of a chromium-contaminated site and to evaluate the differentially expressed proteins of strain VKM014, when exposed to varied concentrations of heavy metal chromium. Sediment samples were collected from the Port of Kakinada situated on the Southern Part of East Coast of India and plated on Nutrient agar medium containing chromate. Strain VKM014 produced both mucoid biofilm and biosurfactant. The strain VKM014 which could tolerate high levels of chromate (0.75 mM) was further selected for strain characterization, growth kinetics and expression of chromate-induced proteins. Based on morphological, biochemical characteristics and phenogram, the isolate was tentatively grouped under Pseudomonas sp. and identified as Pseudomonas stutzeri based on 16S rRNA gene sequence analysis (GenBank EF079450). This study results indicated that chromate upshock resulted in induction/enhancement of two unidentified 25 and 28 kDa induced proteins in the cell extract of the VKM014 following the exposures to 0.2 and 0.5 mM of chromate. In contrast, changes at biochemical level (induced proteins) are the first detectable responses to environmental perturbations and so the sensitive indicators of pollution. This study demonstrates new links between biosurfactant production, differential subpopulation response and metal exposures and involvement of certain enzymes in chromate resistance.
  P.V. Bramhachari , B. Anand Kumar , K.V. Deepika and S. Gnanender
  Microorganisms play a pivotal role in biogeochemical transformations and hence termed as natural decontamination agents. Therefore, screening for tributyltin (TBT) resistant and degrading bacteria is relevant for selection of isolates with decontamination ability of TBT polluted areas. With this rationale, 105 strains were isolated from sediment and surface waters of Vishakapatnam shipping harbor and their tolerance to TBT was evaluated. Further screening was done based on the ability of bacteria to grow in Minerals Medium (MM) containing 3 mM TBT as sole source of carbon. Ten selective TBT resistant isolates showed cross tolerance to six heavy metals and ten antibiotics. Among them, one interesting isolate VBAK101 showing highest TBT resistance (4 mM) and maximum growth yield (A600 nm of 1.6 after 36 h) was selected for further study. The taxonomic identification of strain VBAK101 strain was done by sequencing of 16S rRNA gene. The maximum-likelihood algorithm showed that it formed a coherent cluster with the clad that comprised of Alcaligenes sp., to be its closest phylogenetic neighbor. Under TBTCl stressed condition, VBAK101 produced more exopolysaccharide (EPS) 92 μg mL-1) compared to control conditions (82 μg mL-1). Overall, synthesis of EPS in bacteria is a protective barrier against TBTCl stress. These results suggest that Alcaligenes sp. VBAK101 is potentially useful for the bioremediation of TBT contamination.
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