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Articles by P.U. Krishnaraj
Total Records ( 3 ) for P.U. Krishnaraj
  A. Vikram , A.R. Alagawadi , H. Hamzehzarghani and P.U. Krishnaraj
  The aim of present research is to isolate phosphate solubilizing bacteria and study their relationship with physical, chemical and biological properties of the soil. Phosphate Solubilizing Bacteria (PSB) were enumerated in 66 soil samples collected from eleven districts of Northern Karnataka. It revealed the presence of PSB in 31 soil samples and indicated a strong positive correlation to exist between total bacterial populations and the population of PSB. The organic carbon and available N content of soils also showed a significant positive correlation while soil pH, available P and total P showed a positive but non-significant correlation with that of PSB population. Thirty one PSB containing soil samples were grouped based on their similarity in bacterial population composition using cluster procedure of SAS and population of total and phosphate solubilizing bacteria as similarity measures. Five soil classes were created and subjected to ANOVA to find any significant effect of soil characteristics. Cation exchange capacity, exchangeable Ca (both in cmol kg-1) and clay content showed significant differences between different soil unsupervised formed groups. A total of 55 PSB were isolated from the rhizosphere soil samples collected from different crops grown in vertisols of Northern Karnataka. These isolated PSB strains will be identified and efficient strains will be selected and used for further studies.
  A. Vikram , H. Hamzehzarghani , A.R. Alagawadi , P.U. Krishnaraj and B.S. Chandrashekar
  Phosphate Solubilizing Bacteria (PSB) isolated from the crops grown in vertisols were tested for the production of Plant Growth Promoting Substances (PGPS) such as Indole Acetic Acid (IAA), Gibberellic Acid (GA) and organic acids. All the 30 isolates of PSB were able to produce both IAA and GA and the amount ranged from 1.1 to 28.0 µg/25 and from 0.6 µg to 9.8 µg/25 mL of broth, respectively. The strains PSBV-4 and PSBV-5 produced the maximum amounts of IAA and GA, respectively. The organic acids produced were gluconic acid, tartaric acid, citric acid, maleic acid, succinic acid, glyoxalic acid and a few unidentified acids. The strain PSBV-9 and the standard Pseudomonas striata produced six organic acids while strains PSBV-10, PSBV-25 and PSBV-16 produced only five organic acids. Hierarchical cluster analysis was used to separate PSBs according to IAA and GA production and the PSBs were classified into three major groups. Correspondence analysis was used to find any association between PSB isolates and organic acid production and it showed a significant association between organic acid production and PSB isolates. The PSB strains isolated here have the potential to promote plant growth, as demonstrated here based on the production of PGPS and organic acids. However, their ability to promote plant growth under greenhouse and field conditions has to be validated.
  A. Vikram , P.U. Krishnaraj , H. Hamzehzarghani and K.S. Jagadeesh
  A number of rhizosphere bacterial strains belonging to fluorescent pseudomonads have been used as seed inoculants to promote plant growth and increase yields. A pot experiment was devised as completely randomized factorial design with two main factors to study the effect of application of seven levels of Pseudomonas fluorescens FPD-10 with Bradyrhizobium strains NC-92 and SSP-24 under preincubated and co inoculated conditions (as first main factor) in different time intervals (as the second main factor) with 3 replicates. For each measured response variable (root and shoot biomass, nodule number and biomass and nitrogen content) an analysis of variance (ANOVA) was performed. So the ability of Pseudomonas fluorescens FPD-10 to promote plant growth was assessed under greenhouse conditions using JL-24 variety of peanut as test crop. The inoculation of FPD-10 significantly increased the root dry weight when compared to control. The interaction between FPD-10 and Bradyrhizobium strains NC-92 and SSP-24 were studied under preincubated and coinoculated conditions. The preincubated treatments gave better results than the coinoculated treatments with respect to root dry weight, shoot dry weight, nodule number, nodule dry weight and per cent N content of shoot.
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