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Articles by P.K. Singh
Total Records ( 7 ) for P.K. Singh
  R.D. Ningombam , P.K. Singh and J.S. Salam
  Psophocarpus tetragonolobus (L.) DC. (Winged bean), a lesser known nutritious leguminous plant is grown luxuriantly in Manipur State, North-east India. Almost all the plant parts; leaves, flowers, immature pods, matured seeds and tubers are edible. Winged bean plant parts viz., seeds (tender, matured and fully matured seed), pods case (tender, matured and fully matured pod case) and tubers were chemically analysed on dry weight basis. The concentration of crude protein, fat, and fibre, total sugar, reducing and non-reducing sugars, starch, total amino acid and minerals (Ca, K, Mg, Fe, Zn, Mn, Cu and Co) were analysed. The results indicated that the highest crude fat (1.7%) was present in mature seed and crude protein was present in fully mature seed (50.7%). The maximum amounts of total sugar (488.90 mg g-1), non-reducing sugar (415.95 mg g-1) and starch (420.60 mg g-1) were recorded in tuber. The plant was also found to have significant quantity of minerals. As regard to the mineral content, mature pod case showed the maximum amount of K (8.9 mg g-1), Ca (8.06 mg g-1) and Mg (5.72 mg g-1). Thus, among the stages taken for analysis, mature pod case contains the maximum amount of macro- and micro-elements. So P. tetragonolobus has got a great future prospect, if properly exploited may serve as a supplementary source of protein and minerals, as a subsidiary food material in Manipur.
  V.R. Pandey , P.K. Singh , O.P. Verma and P. Pandey
  Crop yield mostly depends on net result of yield contributing traits, action of genes and their interactions with environment. Hence, an experiment was undertaken with fifty two rice genotypes to know genetic relationship of various polygenic traits on yield. All the genotypes were grown in a randomized complete block design with three replications during Kharif 2009 under salt affected soil (pH of 9.2 and EC 1.61 dS m-1). Results showed that substantial amount of variability exists in the entire gene pool for all traits studied validated ample scope of selection. Magnitude of Phenotypic Coefficient of Variation (PCV) along with Genotypic Coefficient of Variation (GCV) was recorded high for biological yield per plant; moderate for grain yield per plant, spikelets per panicle and flag leaf area. However, days to 50% flowering, days to maturity, plant height, panicle length, spikelet fertility (%), 1000-grain weight and harvest-index had low estimates. High heritability coupled with high genetic advance in percent of mean were observed for biological yield per plant, grain yield per plant, spikelets per panicle and flag leaf area while, the 1000-grain weight and harvest-index suggesting preponderance of additive gene action in the expression of these characters. Grain yield per plant exhibited a very strong positive association with biological yield per plant, flag leaf area and spikelet fertility at phenotypic and genotypic level which indicated that these traits were the strongest associates of grain yield per plant. However, significant negative correlation was observed in case of harvest-index with biological yield per plant, and 1000-grain weight with effective tillers per plant. The genetic reasons for this type of negative association may be linkage or pleiotropy. Path analysis identified harvest-index, days to maturity and effective tillers per plant, 1000-grain weight and flag leaf area and panicle length as major direct contributors. Hence, these traits should be given much emphasis on the selection criteria for genetic improvement of rice yield in salt stress environment.
  P.K. Singh and Vijay Kumar
  Fusarium oxysporum f. sp. chrysanthemi (FOC) is one of the most wide spread and destructive pathogen, causing infection to Chrysanthemum plants throughout the world. Qualitative evaluation of 10 FOC isolates was done for their ability to produce fusaric acid, using thin layer chromatography. A variation in FOC isolates was observed and isolate FO-1, FO-2 and FO-3 were very strong producers of fusaric acid. Isolate FO-1 produced highest biomass in liquid medium and radial diameter on solid medium and FO-2 was the slowest. Growth of FOC isolates was recorded maximum on Potato Dextrose Broth (PDB), followed by Czapex Dox Broth (CDB) and FSM-Liquid. All the isolates of FOC showed significantly (p<0.01) higher growth on pH 5 followed by 7, 9, 3 and 11. FOC isolates growth was highest at temperature of 25°C followed by 30, 20, 15, 35 and 40°C. None of FOC isolates showed radial growth at 40 and 45°C. Results of this study has revealed that a temperature of 25°C, pH of 5.0 and Potato dextrose medium was most suitable for the growth of FOC isolates.
  P.K. Singh , S.R. Gaur and M.S. Chari
  A forty-nine day feeding experiment conduced to determine protein requirement of fingerlings of Labeo rohita (average weight 1.83±0.02 g) feeding on four formulated diets with varying protein levels (25, 30, 35 and 40%) using slaughter house waste as the major protein source. In terms of growth, food conversion ratio, protein efficiency ratio, survival and ratios of protein and lipid deposition in muscle, diet containing 30% protein level revealed a significantly (p<0.01) better performance of the fish in comparison with the fish fed on other diets containing lower or higher protein levels. These findings suggest that about 30 percent crude protein with 370 kcal/100 g energy content in diet appears to be sufficient for obtaining optimum growth in Labeo rohita.
  D.B. Barad , B.S. Chandel , A.I. Dadawala , H.C. Chauhan , H.S. Kher , S. Shroff , A.G. Bhagat , S.V. Singh , P.K. Singh , A.V. Singh , J.S. Sohal , S. Gupta , K.K. Chaubey , S. Chakraborty , R. Tiwari , R. Deb and K. Dhama
  Mycobacterium avium subspecies paratuberculosis (MAP) is the causative agent of chronic enteric granulomatous inflammation in animals and is known as Johne’s Disease (JD) or Paratuberculosis. JD, being spectral in nature, presents variable bacteriological, immunological and pathological spectra leading to variable efficacy of diagnostic methods at different points of time during the course of infection. The present study aimed to estimate the incidence of MAP in two important breeds of goats (Mehsani and Surti) from South Gujarat region of India by applying conventional, molecular and serological methods. A total 219 goats were screened and categorized into Group-I (123 Mehsani goats), Group-II (76 Surti goats) and Group-III, (20 Non-descript goats). Percent positivity by faecal smear examination, delayed type hypersensitivity (DTH), agar gel immunodiffusion (AGID), IS900 polymerase chain reaction) (PCR) and indigenous enzyme linked immunosorbent assay (ELISA) kit was 9.2 (7/76), 21.9 (27/123), 10.9 (24/219), 12.5 (5/40) and 43.3% (95/219), respectively. Of the 123 goats of Group-I, 27 (21.9%) were positive in DTH test. Of the 5 faecal positive goats which also showed clinical signs, 2 (3.5%) goats died during study were negative by Johnin test. Similar to these findings, sensitivity of Johnin test in goats ranged between 18-30% with least specificity in both preclinical and advanced stage of disease. Of 34 cases of caprine paratuberculosis, 73.5% goats were positive for Johnin test. In the present study, out of the 5 infected goats, 3 (60%) were positive in Johnin test. Rectal pinch smear examination was carried out in 27 DTH positive goats and all smears were negative for the presence of acid fast bacilli. Screening tests (Indigenous ELISA and DTH) showed very high incidence of MAP infection in the goat population. The utility of multiple diagnostic tests is suggested for confirmatory detection and epidemiological diseases investigations of MAP in animals.
  A. Kumar , S.V. Singh , A.K. Srivastava , N.K. Gangwar , P.K. Singh , S. Gupta , K.K. Chaubey , R. Tiwari , S. Chakraborty and K. Dhama
  Johne’s Disease (JD), caused by Mycobacterium avium subspecies paratuberculosis, is endemic in domestic animals and adversely affects dairy industry worldwide. In the present study, efficacies of ‘Indigenous’and commercial (Gudair, Spain) vaccines were evaluated for control of JD in experimentally challenged goats. Goats were grouped into Sham-immunized, Indigenous and Gudair vaccine groups. Vaccinated kids were challenged at 50 and 270 Days Post Vaccination (DPV), with 3×109 and 5×109 ‘Indian Bison Type strain ‘S 5’, respectively and sacrificed at 150 and 450 DPV after 1st and 2nd challenge, respectively. Vaccines were evaluated for improvements in physical condition (diarrhea, weakness, body coat color), clinical symptoms (shedding of bacilli, mortality, morbidity), immune responses (cell-mediated and humoral), pathology (gross and microscopic lesions) and production status (body weights, growth rates). Vaccinated goats gained higher body weights vis a vis sham-immunized. Mortality was higher in sham-immunized. Cell Mediated Immunity (CMI) response increased at 30 DPV and showed down regulation from 90 DPV onwards in vaccinated goats. Significant increase in humoral immune response was observed in vaccinated goats at 180 DPV and maintained till 450 DPV. Microscopical examination at 180 DPV showed reduced shedding in vaccinated groups, At 200 DPV, group 1 goats showed thickening of small intestine with corrugations specifically at ileocaecal junction, catarrhal enteritis with infiltration of mononuclear cells and epitheloid cells. In vaccinated groups, there were focal thickening of intestines at 450 DPV with lesions of chronic catarrhal enteritis and presence of lymphocyte, plasma cells and macrophages cells with a few epitheloid cells. Monitoring of MAP DNA in the blood of experimental goats of all the groups was done by testing of blood samples by Polymerase Chain Reaction (PCR) and the vaccinated groups of goats revealed MAP bacilli free status upto 300 DPV. Both the vaccines provided protection after challenge I, but since indigenous vaccine also protected goats after challenge II, was therefore superior. In conclusion, the indigenous vaccine must be exploited for its full potential for effective prevention and control of this economically important disease having public health concerns.
  S.V. Singh , A.V. Singh , P.K. Singh , B. Singh , A.S. Ranjendran and N. Swain
  Study aimed to recover and genotype Mycobacterium avium subspecies paratuberculosis isolates from free-ranging wild bison (Bos gourus) found in the Nilgiri hills of Kodai Kanal region of Tamil Nadu (South India). Of the 13 fecal samples screened for the presence of MAP infection, 6 (46.1%) and 8 (61.8%) were positive in microscopic examination and culture, respectively. Further characterization and genotyping of cultures, using IS900 PCR and IS1311 PCR-REA, study first time revealed presence of Indian Bison Type genotype in the bison population of India. More studies may provide link on the evolution of Indian Bison Type genotype which has been widely reported from domestic ruminants in India. Control of MAP infection in bison will be crucial to check transmission to domestic ruminants and conserve fast dwindling population of wild in India.
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