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Articles by P.K. Mishra
Total Records ( 6 ) for P.K. Mishra
  D. Kumar , J.P. Pandey , A.K. Sinha , S. Salaj , P.K. Mishra and B.C. Prasad
  The Antheraea mylitta produces tasar silk having massive demand in international market. Its rearing is carried out in outdoor conditions and during course of I to III instar rearing, a major proportion (20-30%) of larvae die due to vagaries of nature, pests and predators etc. which considerably affects the production and productivity. The solution to this major bottleneck of the silkworm rearing lies in evolving a suitable tasar silkworm feed (semi-synthetic diet) for young age tasar silkworm. In present study, comparative evaluation was done among semi-synthetic diet fed; fresh leaf fed indoor reared and fresh leaf fed in natural outdoor reared insects. Data revealed that young age survival and Effective Rate of Rearing (ERR) were higher when larvae were brushed on semi-synthetic diet in contrast to indoor rearing on fresh leaf and complete outdoor rearing. Semi-synthetic diet fed larvae showed greater body weight and their cocoon showed higher weight, shell weight and shell ratio than controls. Concentration of hemolymph protein was slightly higher in semi-synthetic diet fed than outdoor reared larvae whereas, significantly lower in case of indoor reared. Hemolymph protein SDS-PAGE analysis indicates that, the semi-synthetic diet fed larvae is closer to complete outdoor reared larvae than indoor reared. Comparative assessment of rearing, cocoon trait and biomolecular profile of A. mylitta it is assumed that tasar feed developed by our laboratory is novel. It will be helpful in minimizing impact of unfavorable condition during I crop rearing and maintenance of precious eco-races stocks to enhance productivity of tasar silk.
  M. Dash , P.K. Mishra and D. Mohapatra
  Mass propagation of sugarcane through meristem culture ensures quick availability of the genetically uniform virus free newly released varieties to the farmers but the occurrence of genetic variation however, limits its commercial application. Isoenzyme analysis may be used to screen somaclones to maintain varietal purity of a sugarcane hybrid like that of a breeder seed in conventionally propagated variety which will be of great benefit to the farmers. Organogenic plant regeneration directly as well as via callus induction from shoot tip of sugarcane indicated that the hormone treatment of KIN (2.0 mg L-1) and NAA (5.0 mg L-1) along with 3% table sugar was best for meristem tip enlargement and shoot initiation. For shoot multiplication a treatment combination of BAP and KIN (1.5 mg L-1 each) was found to be optimum. Highest number of shoots was obtained in treatment combinations of BAP and KIN (1.5 mg L-1 each) along with GA3 (3.0 mg L-1). NAA (5.0 mg L-1) was found optimum with high mean response for rhizogenesis. The detection of genetic variability by analysis of peroxidase isozyme of mericlones and calliclones indicated presence of polymorphism within the mericlones and calliclones. The mericlones exhibited genetic integrity for invertase isozyme while exhibiting the lowest polymorphism for total soluble proteins. The calliclones regenerated after third callus subculture exhibited lowest polymorphism over peroxidase, invertase and total soluble proteins. Thus, micropropagation by callus culture can be advanced up to third subculture to obtain large number of plantlets with minimum variability from that of the parental genotype.
  S. Dutta , P.K. Mishra , G.M. Prasad , S. Shukla and S.K. Chaulya
  Next generation internet protocol IPv6 provides great solution to the problems associated with IPv4. Large address space of IPv6 solves the problem of lack of IP addresses which was a major concern with the enormous growth of the internet. The new version of protocol provides innumerable benefits like auto-configuration, simple header, multicasting, quality of service, etc. which improve the overall functionality of IPv6. In the present study, the benefits of IPv6 on IPv4 have been discussed in detail including the lacuna of IPv6 in present day scenario. The future challenges have also been identified during the implementation of IPv6.
  J.P. Pandey , P.K. Mishra , Dinesh Kumar , A.K. Sinha , B.C. Prasad , B.M.K. Singh and T.K. Paul
  Generally, Antheraea mylitta cocoons cooking is carried out in alkaline condition by using soap, soda, H2O2, etc., which adversely affects the natural beautiful colour and softness of tasar silk. At eclosion stage, the emerging adults of tasar silkworm, A. mylitta exude a proteolytic enzyme ‘cocoonase’ which helps in softening anterior portion of cocoon shell and facilitates emergence of moths. Interestingly, cocoonase directly acts on the sericin protein without affecting the fibroin protein. It evidently indicates that, sericin is excellent natural substrate of cocoonase. This natural phenomenon engenders an idea to collect the cocoonase of A. mylitta and investigate its possible-efficacy in cocoon cooking. The SDS-PAGE analysis of freshly collected cocoonase (from emerging moths) showed molecular weight around 26 kDa. A simple technique for cocoonase collection from freshly pierced cocoons has been developed. Cooking of cocoon in cocoonase is concentration, pH, temperature and time dependent. Low concentration (1:15, 1:20, 1:25, 1:30 and 1:35) increases the cooking time and decreases the cooking efficiency. Higher concentration (1:5 dilutions) minimises the cocking time and increases the cooking efficiency. But cocoons were not fully reeled due to hardness in inner portion of the cocoons. Initial boiling of cocoon in water for 30 min followed by cooking in cocoonase (1:5) at 35-40°C temperature and 8.5 to 9.0 pH yielded comparatively better cooking efficiency with 50-55% silk recovery. Yarn obtained from the cocoons cooked in cocoonase preserve natural beautiful unique tasar silk colour, softness and lustre.
  D. Kumar , J.P. Pandey , J. Jain , P.K. Mishra and B.C. Prasad
  In the present study, quantitative and qualitative changes in protein profile of different tissue of larvae, pupae, adult and eggs of Tasar silkworm Antheraea mylitta Drury was investigated. Stage and age dependent variation in protein concentration and SDS-PAGE protein profile of 36 and 64 kDa protein was observed in different tissue. The concentration of protein was recorded higher in eggs laid by fresh moth than 3 days old moth and significant variation was also noticed in normal and depressed eggs. Interestingly, substantial changes in SDS-PAGE protein profile was observed in normal and depressed eggs and eggs laid by fresh moth than 3 days old moth. Haemolymph and midgut protein concentration was recorded higher in 3rd and 5th instar feeding larvae and in 4th instar mature larvae. Concentration of protein in the haemolymph of pupae before the brain window becomes opaque was higher in both the sexes than opaque stage. Fat body protein concentration in larvae showed increasing trend from 3rd to 5th instar larvae and it was higher in pupae after the brain window becomes opaque and fresh moth. In addition, higher protein concentration was recorded in gonads of pupae after the brain window becomes opaque and in reproductive organs of fresh moth. Present findings would promote to further understand the precise reason for depression of eggs and changes in protein profile in different tissue of A. mylitta.
  J.P. Pandey , Dinesh Kumar , Sony K. Roy , P.K. Mishra , A.K. Sinha and B.C. Prasad
  Tropical tasar silkworm, Antheraea mylitta Drury DABA-ecorace is commercially exploited in India for tasar silk production. Generally, its cocoons used to preserve in outdoor and indoor conditions. But in recent past, due to global warming, tasar silk zone day time temperature (May-June) shoots up and it goes up to 35 to 45°C or even more. It cause adverse impact on the survival of tasar silkworm pupae and invite heavy loss to the tasar silk industry. In the present study, hemocyte and enzyme-based methods have been used to evaluate the impact of outdoor and indoor seed cocoon preservation on A. mylitta pupae. Interestingly, differences in Total Hemocyte Count (THC), hemocyte contour/morphology, protein concentration, fat body and hemolymph acid phosphatase (Acp) activity of outdoor and indoor preserved pupae have been observed. Marked variation in THC was observed among high, low and median weight pupae. Although, no significant variation was observed in THC of indoor and outdoor preserved female pupae but significant difference was found in male pupae of both the preservation conditions. Differential profile of hemocytes was differing in outdoor and indoor conditions with change in cell-contour. Elevation in immune cell “plasmatocytes (PLs)” numbers was recorded in indoor preserved cocoon. Acp activity in fat body of indoor preserved pupae was higher than outdoor but reverse trend was found in hemolymph. Activity profile Acp vary in male and female with higher, lower and median weight pupae. Protein concentration in hemolymph of outdoor preserved pupae was more in comparison to indoor but this trend was found usually reverse in fat body. In addition, less protein concentration was recorded in fat body and hemolymph of male pupae in contrast to female. It is expected that, based on hemocyte and enzyme-based method impact of cocoon preservation conditions on A. mylitta pupae can be evaluated/observed.
 
 
 
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