Asian Science Citation Index is committed to provide an authoritative, trusted and significant information by the coverage of the most important and influential journals to meet the needs of the global scientific community.  
ASCI Database
308-Lasani Town,
Sargodha Road,
Faisalabad, Pakistan
Fax: +92-41-8815544
Contact Via Web
Suggest a Journal
 
Articles by P.K. Lo
Total Records ( 1 ) for P.K. Lo
  P.K. Lo , C.Y. Tan , O. Hassan , A. Ahmad , N.M. Mahadi and R.M. Illias
  In the study presented, Design of Experiments (DOE) was combined with statistical analysis such as fractional factorial design and small central composite design Response Surface Methodology (RSM) to significantly increase the extracelullar recombinant CGTase yields in fermentation flasks. The new medium obtained by the statistical analysis for the significant medium components comprised of 12 g L-1 NZ Amine A, 24 g L-1 yeast extract, 9.44 g L-1 KH2PO4, 4.4 g L-1 K2HPO4, 4.58 mL L-1 glycerol, 7 mg L-1 sucrose and 3 mg L-1 CuCl2. Yields were improved about 68% from 9.54 to 16.07 kU mL-1 in flasks when using the optimized cultivation medium. The results suggest that the overexpression level of recombinant CGTase excreted into the culture medium using the recombinant Escherichia coli could be improved through medium optimization.
 
 
 
Copyright   |   Desclaimer   |    Privacy Policy   |   Browsers   |   Accessibility