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Articles by P.K. Chauhan
Total Records ( 6 ) for P.K. Chauhan
  R.K. Sharma , P.K. Chauhan and A. Fulia
  Endosulphan is a xenoestrogen that imitates the effect of estrogens, causing reproductive and developmental abnormalities in mammals. The aim of the present investigation was to analyze the effect of vitamin E in rescue of degenerating changes induced by endosulphan in granulosa cells of goat Capra hircus in vitro. On the basis of colour and texture normal follicles (3-5 mm in diameter) were selected for the tissue culture. The follicles were divided into two groups (one control+two experimental groups). Experimental group (A) was exposed with 100 nmol mL-1 endosulphan concentration. Experimental group (B) was exposed with 100 nmol mL-1 endosulphan as well as supplemented with 100 μmol L-1 concentration of vitamin E (α-Tocopherol). Harvesting was carried out after 1 h, 4 h and 8 h of exposure. Control was run simultaneously along with all the experimental groups. Endosulphan at dose level 100 nmol mL-1 induced a decline in cell diameter from 7.5±0.0456 in control to 4.5±0.1024, 3.7±0.1001 and 3.2±0.1154 μm after exposure of 1, 4 and 8 h, respectively but in case of endosulphan supplemented with vitamin E, there was less decline in cell diameter that was 6.4±0.1235, 4.8±0.1809 and 4.1±0.0809 μm after exposure of 1, 4 and 8 h, respectively. Endosulphan induced atretogenic changes like hyalinization of granulosa cells, crinkled and wavy membranes and pycnosis and thus affects the functions in adult goat due to the oxidative stress. Vitamin E treatment at dose level 100 μmol L-1 in experimental group (B) these atretogenic changes were milder and restore the normal structure of granulosa cells.
  R.K. Sharma , A. Fulia and P.K. Chauhan
  During the present investigation effect of vitamin C in rescue of degenerating changes induced by endosulphan have been observed. Small pieces of testicular tissue (approximately 1 mm3) were divided into three groups (One control group + two experimental groups). Experimental group (A) was treated with 100 nmol mL-1 endosulphan concentration and experimental group (B) was supplemented with 100 nmol mL-1 endosulphan and 1000 μmol L-1 concentration of vitamin C (Ascorbic acid) and harvesting was carried out after 1, 4 and 8 h of exposure duration. Control was run simultaneously along with all the experimental groups. In the experimental group (A) treated with endosulphan there was alteration in histoarchitecture of seminiferous tubule. Endosulphan exposure after 1 h induced elevation in atretic spermatocytes from 22% in control testicular tissue to 54%, from 28 to 68% after 4 h and from 32 to 74% after 8 h of exposure duration. Testicular tissue exposed with endosulphan and supplemented with Vitamin C at dose level 1000 μmol L-1 in experimental group (B) restore the normal structure of the seminiferous tubule because of its scavenging property to scavenge the free radicals produced due to the oxidative stress. Due to the supplementation of vitamin C along with the exposure of endosulphan to the testicular tissue, atretic spermatocytes were declined from 54% in endosulphan exposed tissue [experimental group (A)], to 32% in vitamin C supplemented group [experimental group (B)] after 1 h, from 68 to 42% after 4 h and from 74 to 54% after exposure duration of 8 h.
  R.K. Sharma , A. Fulia and P.K. Chauhan
  The aim of the present study was to investigate the ameliorating effect of vitamin C on atrazine induced testicular toxicity in Capra hircus in vitro. Small pieces (approximately 1 mm3) of testicular tissue were divided into three groups (One control and two experimental groups). One experimental group was supplemented with 100 nmol mL-1 atrazine concentrations and another experimental group was treated with 100 nmol mL-1 atrazine and simultaneously supplemented with 1000 μmol L-1 concentration of vitamin C (Ascorbic acid). Controls were run simultaneously along with all the experimental groups. Harvesting of tissue was carried out after 1, 4 and 8 h of exposure. In the experimental group treated with atrazine at dose level 100 nmol mL-1, several alterations were observed in the seminiferous tubule. After 1 h of exposure duration there was degeneration in germ cells and somatic cells. Pycnotic nuclei which stained darkly with the eosin were clearly observed after 1 h of exposure duration. The numbers of atretic spermatogonia were increased from 24% in control group to 60% after 1 h, from 30 to 66% after 4 h and from 36 to 76% after 8 h of exposure duration. Similar atretogenic changes were also observed in the testicular slices cultured in atrazine+ vitamin C but were milder as compared to atrazine treatment exclusively. Reduction in atretic spermatogonia was recorded from 60 to 32% after 1 h, from 66 to 42% after 4 h and from 76 to 50% after 8 h of supplementation of vitamin C.
  R.K. Sharma , P.K. Chauhan and A. Fulia
  The presence of pesticide residues in food, in soils and sediments as well as in run-off water are ubiquitous environmental toxicants that pose a risk to human health. Aim of the present investigation was to study the effect of different doses of atrazine on spermatocytes of Capra hircus in vitro. Small pieces (approximately 1 mm3) of testicular tissue were divided into one experimental and one control group; the experimental group was treated with atrazine (1x10-3, 1.0 and 100 nmol mL-1) and exposed for different durations. Pycnosis, chromolysis and vacuoles of varied sizes and shapes were frequently observed in spermatocytes due to the atrazine exposure. All the degenerating changes increased as the exposure duration was increased from 1 to 4 and 4 to 8 h. The relative frequency of atretic spermatocytes was 30, 38 and 44% after 1, 4 and 8 h, respectively at 1x10-3 nmol mL-1 atrazine concentration. Ultra-structurally, shrinkage in cytoplasm of zygotene primary spermatocytes was distinct and prominent, chromolysis, ruptured membrane of turgid mitochondria and Golgi bodies were scattered in the spermatocyte cytoplasm.
  A. Fulia , P.K. Chauhan and R.K. Sharma
  During the present investigation ameliorating effect of vitamin E on endosulphan induced testicular toxicity has been analyzed in Capra hircus in vitro. Vitamin E exhibited the protective role against the damage induced by endosulphan in the testicular tissue. Small pieces (approximately 1 mm3) of testicular tissue were divided into three groups (One control and two experimental groups). One experimental group was treated with 100 nmol mL-1 endosulphan concentration and another experimental group was treated with 100 nmol mL-1 endosulphan and supplemented with 100 μmol L-1 concentration of vitamin E (α-Tocopherol). Harvesting of the testicular tissue was carried out after 1, 4 and 8 h of exposure durations in vitro. Hyalinization and fragmentation was observed in the endosulphan treated group. Chromolysis was observed in spermatogonia, Sertoli cells and spermatids. As the exposure duration enhanced from 4 to 8 h there was elevation in number of pycnotic nuclei, fragmented nuclei, chromolysis of germ cells and somatic cells present in the testis. Endosulphan exposure induced the number of atretic spermatogonia from 24% in control group to 68% after 1 h, from 30 to 76% after 4 h and from 36 to 84% after 8 h of exposure duration. In the experimental group treated with endosulphan and supplemented with vitamin E there was decline in number of pycnotic nuclei, fragmented nuclei and chromolysis as compared with the endosulphan exposed group. There was decline in atretic spermatogonia from 68 to 36% at 1 h, from 76 to 44% after 4 h and from 84 to 58% after 8 h of supplementation duration.
  R.K. Sharma , P.K. Chauhan and A. Fulia
  The aim of the present study is to analyze the effect of different doses of endosulphan on spermatogonia of Capra hircus in vitro. Small pieces (approximately 1 mm3) of testicular tissue were divided into two groups (one experimental group and one control group). Experimental group was treated with different concentrations of endosulphan (1x10-3, 1.0 and 100 nmol mL-1) and was further divided into three subgroups (1), (2) and (3) exposed for 1, 4 and 8 h, respectively. The endosulphan exposure revealed increasing morphological alterations with increasing dose of endosulphan in spermatogonia. At dose level 1x10-3 nmol mL-1 of endosulphan, light microscopic changes in spermatogonia were characterized by condensed nuclei which were darkly stained with haematoxylene eosin. Hyalinization and chromolysis, vacuolization in spermatogonia were clearly observed. Vacuolization in spermatogonia were also enhanced as the exposure duration extended. Endosulphan at concentration level 1x10-3 nmol mL-1 induced decline in spermatogonial diameter from 7.245±0.201 in control to 6.75±0.231 μm after 1 h, 6.5± 0.2115 μm after 4 h and 6.45±0.2233 μm after exposure duration of 8 h. At the same dose level of endosulphan atretic spermatogonia were 34, 44 and 52% after exposure duration of 1, 4 and 8 h, respectively. Due to increase in the endosulphan concentration upto 100 nmol mL-1, atretogenic changes induced in spermatogonial cells were characterized by increased fragmentation and pycnosis of nucleus. Ultrastructurally, chromolysis, vacuolization, hyalinization, condensation of nucleus, apoptotic vacuoles and shrinkage of cytoplasm was generally observed. These changes were prominent at higher dose level and extended exposure duration.
 
 
 
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