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Articles by P. Thirunavukkarasu
Total Records ( 9 ) for P. Thirunavukkarasu
  P. Thirunavukkarasu , T. Ramanathan , G. Umamaheshwari , V. Manigandan and P. Dinesh
  Ipomoea pes-caprae is a sand dune plant commonly used as folklore medicine for fisherman communities based on the traditional knowledge. Little information is available on the feasibility of applying micropropagation techniques for production of dune and marsh species. The main aim of this study is to increase the callus induction and shoot generation of sand dune plant Ipomoea pes-caprae with easily available low cost natural materials. Coconut water is the rich source of carbohydrate and other nutrients which enhance callus and plant regeneration. In our present study, we tried different type of MS medium (Full and half strength) with coconut water at three different percentage (10, 15, 20%) and different concentration of plant growth regulators for callus induction and shoot regeneration. Well-developed callus inoculated in full and half strength MS medium with different concentration of CW and plant growth regulators. The best results were accomplished with half strength MS medium with 15% coconut water and with 2, 4-D and IAA 0.7 mg L-1 concentration which shows better callus induction and shoot regeneration. Young shoot and root developed plants transferred to green house and then followed to soil.
  P. Thirunavukkarasu , T. Ramanathan , S. Asha , V. Manigandan and D. Kannan
  Rhizophora apiculata, a mangrove specie is used in traditional medicine against several human diseases including tumor. To validate the ethnopharmacological claims against cancer, we examined the effects of Rhizophora apiculata leaf oil on the anti gastric cancer activities of Benzo(a)pyrene (BaP) induced gastric cancer in albino mice. The animals were divided into five groups. Group I were given BaP (40 mg kg-1 b.wt.) with 100 μL sesame oil by oral gavage twice a week for 4 consecutive weeks. Group II were given 100 μL sesame oil (SMO) treated (Vehicle treated control) a week for 4 consecutive weeks. Group III mice (BaP) (40 mg kg-1 b.wt.)+R. apiculata (50 μL) for 14 weeks. Group IV only R. apiculata (50 μL kg-1 b.wt.) dialy for two weeks. Goup V as the control non treated. At end of 14 weeks, all the animal were killed. Tumor incidence was observed to be 100% in mice that received only BaP. However, treatment with R. apiculata oil reduced the tumor incidence as observed in mice of BaP+R. apiculata oil group when compared to that of BaP group. Similarly, the tumor burden and body weight were seen to decrease by 137.21, respectively in mice of BaP+R. apiculata oil group when compared to those of BaP group. Diminished lipid peroxidation in the stomach tumor tissue was associated with enhanced antioxidant levels. In contrast to tumor tissue, enhanced lipid peroxidation with compromised antioxidant defences was found in the liver and erythrocytes of tumor bearing animals. Administration of R. apiculata oil significantly reduced the incidence of stomach tumors, modulated lipid peroxidation and enhanced antioxidant status in the stomach, liver and blood. From the results of our study, we suggest that R. apiculata oil may exert its chemopreventive effects by modulating lipid peroxidation and enhancing the antioxidant status in the stomach, liver and erythrocytes.
  L. Ramkumar , T. Ramanathan , P. Thirunavukkarasu and N. Arivuselvan
  This study revealed that antioxidant properties, 1, 1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity and reducing power were observed in different mushroom species namely Agaricus bisporus, Pleurotus sajor-caju, Pleurotus euos, Hybsizus ulmarius, Pleurotus florida, Volveriela volvaciea, Pleurotus platypus, Pleurotus djamor and Calocybe indica. Methonolic extract of P. euos was recorded maximum levels of DPPH free radical scavenging (6 mg mL-1), reducing power (6 mg mL-1) and enzymatic antioxidants viz., catalase, superoxide dismutase and peroxidise, 68.7±1.1, 0.39±0.01, 42.21±0.06, 37.12±0.04 and 7.21±0.05, respectively. The maximum amount of enzymatic antioxidants were recorded viz., CAT, SOD and POX; 42.21±0.06, 37.12±0.04 and 7.21±0.05 μmol, respectively.
  P. Thirunavukkarasu , M. Moorthy and K. Viswanathan
  A study was carried out to find out the effect of induced moulting of commercial SCWL laying hens at 60, 65 and 70 weeks of age group. A control group was kept without moulting at their respective ages. The layers were reared in cages for the entire experimental period adopting standard managemental practices. The layers fed commercially available layer diet. After 28 days period of egg production (pre-moult), the layers were subjected to induced moulting by feed and water restriction followed by ad-libitum feeding and had free access to wholesome water. Seventeen hours photoperiod was provided daily through out the post-moult laying period. The birds in 70 weeks age group had higher livability among the moult induced groups and no change in body weight.
  D. Kannan , K. Jagadeesan , N. Shettu , A. Gopalakrishnan and P. Thirunavukkarasu
  A quantitative study was made on the occurrence of cholesterol in the muscle of different maturity stages of the wilder tiger shrimp Penaeus monodon collected from Pazhayar coast, Nagapattinam, Tamil Nadu. As the size increased from 8-10 cm to 14-16 cm the cholesterol content increased from 1.4-4.5 mg g-1. The shrimp contained almost the same level of cholesterol in both sexes. The variation in the cholesterol level in relation to moulting in the male and female shrimp revealed that in both the sexes high cholesterol content was noticed in the intermoult stage. Subsequently, in the premoult stage the cholesterol content decreased from 2.0-1.8 mg g-1 and it was found to be the lowest among all the moult stages. In the matured stages the shrimp contained higher level of cholesterol (2.1 mg g-1) and very less amount of cholesterol level (1.2 mg g-1) in the immature and early mature stages. It is inferred from this study that the cholesterol level was more in the intermoult stage suggesting that cholesterol may be converted to some moulting hormones.
  P. Thirunavukkarasu , T. Ramanathan , N. Ravichandran and L. Ramkumar
  The objective of this study was to determine the potential anti-microbial activity of aqueous ethanolic and chloroform extracts from 3 medicinal plants against bacteria and some fungal strains. The antimicrobial efficacy of Citrullus colocynthis, Citrullus lanatus and Citrullus vulgaris, was evaluated against Gram negative, positive and some fungal strains and including Esherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Bacillus subtilis and Bacillus pumilus some fungal Candida albicans, Aspergillus niger, Penicillium chrysogenum and Penicillium chrysogenum using agar well diffusion method at concentration levels (100 mg mL-1). Among the tested plants, Citrullus colonthis extract showed the highest antibacterial and anti fungal activity and all bacterial and fungal strains at 100 mg mL-1. In addition, almost all species of plants were found to have activity on at least two microbial strains. However, the diameters of inhibition zone were different according to the kinds and concentrations of plant extracts and bacterial and fungal strains. The ethanol extract of citrullus colocynthis was very active against bacterial and fungal at a concentration of 100 mg mL-1. But Trichosporon begelli showed that no zone inhibition in C. valgaries plants of ethanol and chloroform extracts.
  D. Kannan , K. Jagadeesan , N. Shettu and P. Thirunavukkarasu
  The Penaeid shrimp commonly known as tiger shrimp is commercially important candidate species for brackish water aquaculture in India. The spawning seasons of penaeid shrimp, P. monodon from Pazhayar coast were studied on the basis of ovarian colour. During maturation shrimp ovaries pass through a series of colour changes. Females with olive green i.e., black green with butterfly band shaped ovaries considered as fully ripening one which is ready to spawn. The presence of fully ripened ovaries of P. monodon throughout the year suggests that these shrimp have the potential for spawning all the twelve months in a year. However, P. monodon noticed with two spawning peaks viz., March and August. Among these two seasons, the highest number of stages with maturity was noticed in the month of March.
  P. Thirunavukkarasu , T. Ramanathan and L. Ramkumar
  Acanthus ilicifolius is a very potential plant and it has more phyto chemical compounds. In present study, anti microbial and hemolytic effects of Acanthus ilicifolius leaves extracts of chloroform and aqueous in isolated protein. The antimicrobial activity of crude extract against bacterial and fungal pathogens showed the clear inhibition zone against Vibrio cholerae and Aspergillus niger in chloroform extract and aqueous extracts showed clear inhibition zone for Pseudomonas sp. and Candida albicans. Both the extracts exhibited hemolytic activity which was estimated as 10.80 ht mg-1 for chloroform extract and 9.5 ht mg-1 for aqueous extract. The partial purification of protein is done by using DEAE cellulose. On SDS-PAGE the crude protein yielded three well defined bands at 100.5, 52 and 21.4 kDa in both the extracts.
  P. Thirunavukkarasu , T. Ramanathan , R. Shanmugapriya , G. Umamaheswari and G. Renugadevi
  Acanthus ilicifolius is a mangrove plant and it used coastal village peoples for traditional folk medicine method for varies disease. In general salt tolerance plants have a more antioxidant constitute. In the present study researchers have examine different type of anti oxidant capacity like that total phenolic, DPPH radical, nitraxcite radical, hydroxyl radical and ABC radical in two solvent extract of ethanol and aqueous for different concentration like 0.1, 0.2, 0.5, 1.0 and 2.0. In the result showed that maximum in 2.0 mg mL-1 of leaves extract of ethanol and minimum in aqueous extract in 0.1 concentration for all antioxidant assay. In this result showed that highly effect in antioxidant levels in two solvent extract and varies concentration so there would be a good antioxidant property.
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