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Articles by P. Shayan
Total Records ( 1 ) for P. Shayan
  B. Meshgi , A. Karimi and P. Shayan
  Random amplified polymorphic DNA (RAPD-PCR) was used to study the genetic variation of Fasciola hepatica in sheep, cattle and buffalo. DNA was extracted from adult worms collected from livers of each infected animal in a slaughter house at Gilan province Caspian Sea region of Iran and amplified by the polymerase chain reaction, using four oligonucleotide decamers with arbitrary DNA sequences as primers. RAPD patterns showed the specific but different pattern DNA fragments for each primer. The intraspecific similarity coefficient within three isolates of Fasciola hepatica was ranged between 78 to 100%. Present findings showed that the interspecific genetic distance was higher than intraspecific genetic distances (22-50% compare to 0-22%). Which similarity coefficient between sheep and buffalo strains were the lowest values (50%). The inferred phylogenetic tree on the fingerprinting of these isolates clearly demonstrated the existence of population genetic diversity sub structuring within Fasciola hepatica of sheep, cattle and buffalo of Iran, raising interesting questions on the host specificity, epidemiology (e.g., zoonotic transmission) and ecology of this fluke.
 
 
 
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