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Articles by P. Sharma
Total Records ( 9 ) for P. Sharma
  R. K. Manna , B. B. Satpathy , C. M. Roshith , M. Naskar , Utpal Bhaumik and P. Sharma
  Hooghly estuary, the lowermost part of River Ganges in India, following the construction of Farakka barrage in 1975, witnessed improved freshwater inflow in the estuarine stretch of the river with significant impact on the salinity regime, water transparency, suspended sediments and nutrient load leading to alternations in the distribution and abundance of fish and other biotic organisms like plankton and benthos. A detailed seasonal study was conducted in Hooghly estuary during 2010-2012 to evaluate the spatio-temporal changes in selected physico-chemical parameters like temperature, transparency, pH, free CO2, dissolved oxygen, specific conductivity, total alkalinity, total hardness, salinity, available nutrients like nitrate, phosphate and silicate under the present hydrological regime in determining the distribution of fish and other aquatic organisms. The salinity based zonation during pre-Farakka period apparently stands modified with a downward shift of freshwater zone. Accordingly, distribution and abundance of biotic communities like plankton and fish now present a completely different scenario from the past.
  N.S. Gill , J. Bajwa , K. Dhiman , P. Sharma , S. Sood , P.D. Sharma , B. Singh and M. Bali
  The objective of present study was to evaluate the therapeutic potential of Cucumis melo seeds traditionally consumed and it was studied for free radical scavenging potential by hydrogen peroxide (H2O2) and DPPH method. Anti-inflammatory activity was evaluated using carrageenan-induced paw edema in rats. Analgesic activity was evaluated by tail immersion and tail flick methods in mice. The percentage scavenging of DPPH radical and H2O2 radical was 74.9% at 300 μg mL-1 and 58.91% at 200 μg mL-1 respectively concentration of. The H2O2 scavenging effect of MECM was 58.91%. The percentage reduction of paw edema was 56.6% at the dose of 300 mg kg-1. The high dose caused significant reduction in pain. Thus it can be concluded from the above results that Cucumis melo seeds have potent anti-inflammatory, antioxidant and analgesic activity.
  N.S. Gill , K. Dhiman , J. Bajwa , P. Sharma and S. Sood
  The present study was designed to investigate the free radical scavenging, anti-inflammatory and analgesic potential of methanolic extract of Benincasa hispida seeds (MEBH). Free radical scavenging potential of MEBH was evaluated by DPPH (1,1-diphenyl-2-picryl-hydrazyl) and hydrogen peroxide (H2O2) method. The extract showed significant free radical scavenging activity in a dose dependent manner when compared with ascorbic acid. The highest radical scavenging activity of MEBH was found to be 79.8% at concentration of 300 μg mL-1. The H2O2 scavenging effect of MEBH was 63.7% at a concentration of 200 (μg mL-1). Further, the extract was studied for its anti-inflammatory and analgesic activities at the dose level of 100, 200 and 300 mg kg-1. Anti-inflammatory activity was evaluated using carrageenan-induced paw edema in rats. Analgesic activity was evaluated by tail immersion and tail flick methods in mice. The extract showed significant decrease in paw volume (59.7% reduction) and pain at the dose level of 300 mg kg-1 when compared with reference drug diclofenac and morphine, respectively. The MEBH may be useful as a natural antioxidant in the treatment of inflammation and pain.
  M. Pant , P. Sharma , T. Radha , R.S. Sangwan and U. Roy
  In the analysis of enzyme kinetics data, Km and Vmax play a very important role. Linearization of kinetic equation is still a common practice for determining these parameters. Although graphical methods help in understanding the kinetic behavior of enzymes, they have certain shortcomings associated with them due to which they sometimes lead to an anomalous estimation of the kinetic parameters. In order to yield a more accurate estimate of parameters, minimization of least square error can be quite useful. However, since the least square error determination is a non linear function, the usual methods may not be fruitful. This research recommends the use of two simple and fast evolutionary optimization techniques such as Genetic Algorithms (GA) and Particle Swarm Optimization (PSO) which may be applied for the determination of Michaelis Menten (MM) enzyme analysis. We have shown the working of these methods on a set of six enzymes taken from literature along with a unique enzyme, geraniol acetyltransferase (GAAT), purified from the aromatic grass palmarosa. The entire study shows that GA and PSO can be used efficiently for determining accurate values for Km and Vmax.
  A. Mishra , P. Sharma , Namrata Soni and Ruchita Awate
  Copper and cobalt complexes derived from 3-(N-phenyl)-thiourea-pentanone-2 were characterized by elemental, XRD, FTIR, UV-Vis, SEM and 1H NMR spectroanalytical studies. The X-ray diffraction studies indicate that 3-(N-phenyl)-thiourea-pentanone-2 and complexes with copper and cobalt are crystalline in nature with simple cubic lattice structure. IR spectroscopic data were used to assign characteristic vibrational frequencies of groups present in these compounds. Scanning electron micrograms were used to assign morphology and particle size.
  N.S. Gill , J. Bajwa , P. Sharma , K. Dhiman , S. Sood , P.D. Sharma , B. Singh and M. Bali
  The present study was to investigate the anti-ulcer activity of methanolic extract of Cucumis melo (MECM) seeds. The seeds were extracted with methanol and the extract was evaluated for its free radical scavenging activity by DPPH. It shows maximum antioxidant activity. Thus extract was further evaluated against gastric ulcerations by Pyloric Ligation (PL), Water Immersion Stress (WIS) and NSAID (indomethacin) induced ulcer (NIU) models. In case of PL method, the extract inhibited gastric ulcerations in a dose-related manner (150 and 300 mg kg-1). Then it was evaluated for gastric volume, free acidity, total acidity, ulcerative index. The ulcerative index was also measured in WIS model and NIU with same doses. The MECM caused 57.6, 67.6 and 61.9% inhibition of ulcers in pyloric ligation, water immersion stress, NSAID induced ulcer models respectively at 300 mg kg-1. The results concluded that MECM possess high antiulcer potential which is due to its higher antioxidant activity.
  N.S. Gill , P. Sharma , J. Bajwa , K. Dhiman , S. Sood , P.D. Sharma and M. Bali
  The present study was designed to investigate Cucumis melo var. utilissimus seeds for their antioxidant and therapeutic potential. The antioxidant activity of methanolic extract was measured by DPPH method and hydrogen peroxide free radical scavenging activity. The methanolic extract of Cucumis melo var. utilissimus seeds (MECU) showed maximum antioxidant potential. Hence, it was further evaluated for anti-inflammatory activity by carrageenan induced rat paw edema and analgesic activity by tail immersion method and tail flick method at different concentrations, i.e., 100, 200 and 300 mg kg-1. The significant results was found at maximum dose. The results suggested that methanolic seed extract possess great therapeutic potential.
  N.S. Gill , K. Dhiman , P. Sharma , J. Bajwa , S. Sood , P.D. Sharma , B. Singh and M. Bali
  In the present study free radical scavenging and antiulcer potential of Benincasa hispida seeds was evaluated. The powdered seeds were extracted with methanol. The extract was evaluated for its free radical scavenging activity by DPPH method followed by antiulcer activity using pyloric ligation, water immersion stress and NSAID (indomethacin) induced gastric ulcer model (NIU) in rats. The parameters assessed were gastric volume, free acidity, total acidity, ulcer index and percentage ulcer protection. Ranitidine was used as the reference antiulcer drug. The methanolic extract showed concentration dependent DPPH radical scavenging activity. The methanolic extract of Benincasa hispida seeds (MEBH) inhibited gastric ulceration by decreasing the gastric volume, free and total acidity. The high dose (300 mg kg-1) showed significant reduction in the above parameters which was comparable to the standard drug ranitidine (p<0.05). The MEBH caused 52.7, 67.4 and 61.2% inhibition of ulcers in pyloric ligation, water immersion stress and NSAID induced ulcer models, respectively at 300 mg kg-1. Thus it can be concluded that the seeds of Benincasa hispida possess potent antiulcer activity. The antiulcer action may be exerted through free radical scavenging mechanism.
  R. Singh , S.A. Dar and P. Sharma
  Herbs are largely unexplored source of drug repository. Medicinal plants have great potential for providing novel drug leads with novel mechanism of action. The present study describes the antibacterial activity, phytochemical profile and toxicological evaluation of Urtica dioica. U. dioica leaves were subjected to solvent extraction by hexane, ethyl acetate, chloroform, methanol and water. All the extracts were tested for antibacterial activity against five clinical isolates of Gram positive and Gram negative bacteria by disc diffusion method. Hexane extract showed good antibacterial activity against all the five bacterial strains, hence it was further purified using silica column chromatography and Minimum Inhibitory Concentration (MIC) of the semi purified fraction-B (FB) of hexane extract was determined by serial tube dilution method. FB showed MIC value of 31.25, 250, 7.81, 31.25 and 125 μg mL-1 against Staphylococcus aureus, Pseudomonas aeruginosa, Salmonella typhi, Klebsiella pneumoniae and Shigella flexneri, respectively. FB was subjected to GC-MS analysis in search of potent antibacterial compound(s). Neophytadiene (26.97%) butyl tetradecyl ester (9.53%), Dibutyl phthalate (7.45%), Bis (2-ethyl hexyl) maleate (8.80%) and 1,2-benzenedicarboxylic acid (9.89%) were the major constituents which may be responsible for the antibacterial activity of FB of U. dioica. Sub-acute oral toxicity of FB was carried out in wistar rats at doses of 100, 200, 400 and 800 mg kg-1 Body Weight (b.wt.) to assess index of safety. FB showed MIC value as low as 7.81 μg mL-1 against Salmonella typhi, exhibiting that a promising antibacterial agent is present in this fraction. Hematological and biochemical parameters showed that the FB was safe at tested concentrations. Hence further purification of FB is required to obtained potential antibacterial compound.
 
 
 
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