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Articles by P. Jayasankar
Total Records ( 2 ) for P. Jayasankar
  P.K. Nanda , P. Swain , S.K. Nayak , T. Behera , P. Jayasankar and K. Dhama
  The success in establishing in vitro culture from anchorage dependent tissue explants depends upon their ability to attach to culture substratum. In this study, evaluation of different coating factors on the attachment and subsequent monolayer formation from various tissue explants of Indian Major Carp, Mrigal, Cirrhinus mrigala was investigated to establish cell culture. Explants from heart, liver, gills, testis, ovary, kidney and fin were taken in respective culture flasks coated either with type I collagen, gelatin or fibronectin to know their ability on attachment and proliferation of cells resulting in attaining confluency. The percentage attachment of explants from same tissue, when cultured in different coated flasks, varied greatly. Explants (40-65%) from liver, ovary, testis and fin were found to attach well in fibronectin coated flasks, whereas maximum attachment (>65%) from heart tissue was recorded in gelatin coated flasks. This difference in percentage attachment of explants to substratum significantly affected growth, proliferation of cells and subsequent formation of confluent monolayer. Overall, fibronectin as a coating factor was found to be good for attachment of explants from most of the tissues of C. mrigala. Contrary to this, attachment percentage of explants from fin, gills, ovary and testis in type I collagen coated flasks was less which eventually failed to form monolayer. The findings indicate that coating factor(s) has a major role in establishing cell culture which not only influences attachment but also growth and proliferation of cells from explants obtained from different tissues of C. mrigala. Further investigations are suggested to find out suitable coating factors for each cell types of different fish species so as to establish cell cultures.
  P. Mohapatra , R.K. Swain , S.K. Mishra , T. Behera , P. Swain , S.S. Mishra , N.C. Behura , S.C. Sabat , K. Sethy , K. Dhama and P. Jayasankar
  Comparative study on the effect of nano selenium (nano-Se) and sodium selenite on the growth, bioavailability, antioxidative activities, hematological and biochemical parameters, cellular and humoral immunity was done in layer chicks upto 8th week post feeding. The results showed significant differences (p<0.05) in relative weight gain and final body weight of the nano-Se treated groups upto a dose of 0.3 mg kg-1 of diet as compared to sodium selenite and control groups. However, further increase in dietary nano-Se content in feed had negative effect on weight and Relative Gain Rate (RGR). Survival rate and Feed Conversion Ratio (FCR) were not affected by dietary treatments. Chicks fed with both nano-Se and sodium selenite showed higher (p<0.05) Se content in different tissues (breast muscle, liver, kidney, pancreas, serum and feathers). However, highest value (p<0.05) of Se content in breast muscle and liver was observed in nano-Se treated groups. Selenium concentrations in serum, liver and breast muscle increased linearly and quadratically (p<0.05) as dietary Se level increased for all Se sources but its magnitude was substantially greater (p<0.05) when nano-Se was fed. Glutathione peroxidase (GSH-Px), erythrocyte catalase (CAT) and superoxide dismutase (SOD) activities were significantly different (p<0.05) in all treated groups than control. Dietary nano-Se also increased several serum biochemical and haematological parameters. In addition, it significantly increased both cellular and humoral immunity in layer chicks after 8th weeks of post feeding. In conclusion, dietary administration of nano-Se was found superior than that of inorganic sodium selenite in various aspects in layer chicks. Further extensive study for exploring absorption mechanisms, metabolic pathways, ideal dose/form of nano-Se is suggested for optimum utilization of nano-material based application of Se feeding in poultry.
 
 
 
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