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Articles by P. Jaisil
Total Records ( 6 ) for P. Jaisil
  C. Bounphanousay , P. Jaisil , J. Sanitchon , M. Fitzgerald , N.R. Sackville Hamilton and J. Sanitchon
  Fifty three accessions of lowland Black Glutinous Rice (BGR) and three white rice varieties from Lao PDR were evaluated for variations in 2-acetyl-1-pyrroline (2-AP), a main aroma compound present in fragrant rice and a 257 bp genomic fragment associated with a gene encoding 2-AP. The objective of this study was to characterize the variation in aromatic characters of Lao BGR by using chemical and molecular techniques. Thirty six out of the 56 accessions had aromatic properties, containing 2-acetyl-1-pyrroline (2-AP). The concentrations of 2-AP in unpolished samples were about 50% higher than those of the polished samples. The concentrations of 2-AP in BGR accessions were lower than those of white aromatic rice varieties in which 2-AP was highest in Kai Noi Leuang (0.688 ppm). Seventeen accessions and two of white rice (Kai Noi Leuang and TDK5) had a common 355 bp allele corresponding to that found in Calrose, a non-fragrant variety. A 257 bp allele was found in the 36 varieties of BGR and a local aromatic white rice variety, Hom Nang Nouan, sharing a common allele as in the fragrant variety KDML 105. Surprisingly, Kai Noi Leuang, a popular Lao local aromatic rice variety with the highest 2-AP concentration did not possess a 257 bp fragment which has been identified previously as a fragrant allele. This is the first study of the chemical and molecular aromatic properties of lowland BGR varieties of the Lao PDR and the data obtained will be valuable in screening other germplasm and in planning a breeding program in the country geared towards fragrant rices with superior agronomic properties.
  C. Bounphanousay , P. Jaisil , K.L. McNally , J. Sanitchon and N.R. Sackville Hamilton
  The genetic diversity of 74 genotypes, including Black Glutinous Rice (BGR) from Lao`s germplasm was assessed using 24 microsatellite markers. A total of 75 alleles were detected at the 24 microsatellite markers. The number of alleles per marker varied from 2 to 7 with an average of 3.1 alleles per locus. The Gene Diversity (GD) and Polymorphism Information Content (PIC) ranged from 0.18 to 0.79 and 0.17-0.76, respectively and the Allele Frequency (AF) ranged from 0.36 to 0.90. The markers were able to classify rice genotypes into four groups; indica rices were put in the three groups while the other group consisted of tropical japonica rice. The first indica group (G1) included 24 genotypes of BGR and five genotypes of white rice. Most of genotypes in this group have thick culms, broad leaf blades, large and bold grain shapes and some of them have purple coloration on all vegetative parts. In other varieties with black pericarp, all other plant parts are green. Indica group 3 (G3) included 25 genotypes of BGR. The special characters of this subgroup were small and slender culms, narrow short leaves, purple leaf margins, purple leaf tips and purple stripes on leaf blades and sheathes. However, the markers used could not differentiate between LG 8215 and LG 7937. Indica group four (G4) consisted of eight genotypes of white rice and the four check varieties. Group (G2) consisted of five white rices, four BGRs and three check varieties.
  N. Singkham , S. Jogloy , T. Kesmala , P. Swatsitang , P. Jaisil , N. Puppala and A. Patanothai
  Oleic acid in peanut oil increases shelf-life and kernel quality. Heritability for oleic acid is important for predicting selection progress. The aims of this study were to estimate the heritability of oleic acid content by parent-offspring regression and to determine the correlation among oil characters. The data were collected in the F2 and F3 generations derived from the crosses between two high-oleic peanuts (SunOleic 97R and Georgia-02C) and a low oleic peanut (KKU 1). The F2 populations were planted in the rainy season (2008) and the F3 populations derived from the F2 populations were planted in the dry season (2008/09) at Khon Kean University. Heritability estimates in narrow sense were intermediate to high for oleic and linoleic acids (0.63-0.72 and 0.57-0.72, respectively). Heritability estimates for % oil were low in all populations. Significant and negative correlation was observed between oleic and linoleic acids (r = -0.98). The high heritability for oleic acid in this study indicated that selection for high oleic acid in peanut was effective in the F2 generation.
  N. Jangpromma , P. Songsri , S. Thammasirirak and P. Jaisil
  In vitro assessments of chlorophyll content are expensive, laborious and time consuming. The alternative methods which are more rapid and straightforward could be very useful. The aim of this study was to investigate the effects of water stress on chlorophyll content and SPAD Chlorophyll Meter Reading (SCMR) and the relationship between chlorophyll content and SCMR under well-watered and drought conditions. Ten sugarcane genotypes (Uthong 6, Khon Kaen 80, K86-161, Khon Kaen 3, 03-4-425, KU60-1, Phill.66-07, B34-164, Uthong 2 and LF82-2122) and two water regimes (well-watered control and water stress at early growth stage) were laid out in factorial experiment in a randomized complete block design with two replications. Drought was imposed to the crop for 10 days during 90 days after transplanting (DAT) to 100 DAT. Data were recorded for total chlorophyll content by N, N-dimethylformamide extraction and SCMR on the second fully expanded leaf from the top at 90 DAT, 100 DAT and 110 DAT. Drought significantly reduced chlorophyll content and SCMR. The reduction in chlorophyll content was more severe than SCMR. Similar responses of sugarcane genotypes to drought and well-watered conditions were observed for chlorophyll content and SCMR. The breeding line 03-4-425 and the cultivars KU60-1 were identified as the best genotypes for chlorophyll content and SCMR. The correlation coefficients between chlorophyll content and SCMR were significant under well-watered conditions and the relationships were more strong and consistent under drought and drought relief. The SCMR can be used for evaluation of chlorophyll content under different water regimes.
  C. Jogloy , P. Jaisil , C. Akkasaeng , T. Kesmala and S. Jogloy
  The rapid accumulation and conversion of the dry matter into harvestable yield under limited crop duration are preferable for peanut (Arachis hypogaea L.) grown in various cropping systems where intensified cropping systems are generally practiced. In these cropping systems, peanut genotypes with acceptable yield and shorter crop duration are required. The objectives of this study were to estimate the broad sense heritability for Crop Growth Rate (CGR), Pod Growth Rate (PGR), Partitioning Efficiency (PC) and reproductive duration in an advanced generation of segregating population of large-seeded type peanut and to investigate the relationship among these characters. Two-hundred breeding lines in the F6 generation of 10 peanut crosses (twenties for each cross) were assigned in a randomized complete block design with two replications. CGR, PGR, PC and RD were recorded at harvest. Heritability estimates for CGR (0.00-0.60) were lower than those for PGR (0.04-0.68), PC (0.00-0.82) and RD (0.17-0.90). Correlation coefficients among CGR, PGR and PC were positive and significant, whereas they were negatively and significantly correlated with RD. The results suggested that improvement of CGR, PGR, PC would be possible among studied materials and would result in lower reproductive duration and early maturity.
  A. Poledate , S. Laohasiriwong , P. Jaisil , N. Vorasoot , S. Jogloy , T. Kesmala and A. Patanothai
  The objective of this study was to determine relative importance of gene effects for PBNV incidence and PBNV severity evaluated at 30, 40, 50 and 60 days after planting. Eight generations of three crosses involved three parental lines were evaluated for disease incidence (percentage of infected plants) and disease severity under natural occurrence of PBNV infection in a randomized complete block design with six replications. Evaluations were carried out at 30, 40, 50 and 60 Days After Planting (DAP). The analysis followed Hayman’s model and Gamble’s notations were used to describe parameters of gene effects. Joint scaling test was used to determine adequacy of the model. Additive gene effect was the most important contribution to genetic variation in generation means for both disease incidence and disease severity in the cross ICGV 86388 x IC 10. Selection for lower disease incidence and disease severity in this cross is promising. Additive gene effect and additive x additive epistatic gene effect were also important but in lower magnitude in the cross ICGV 86388 x KK 60-1 for disease incidence at 60 DAP. The presence of significant dominance gene effect in this cross for disease incidence might hinder the progress from selection. The consistent and significant additive gene effect for disease severity might provide a better selection strategy. Additive gene effect was significant for disease incidence only in the cross IC 10 x KK 60-1 at 60 DAP. Additive x dominance epistatic gene effect was also significant at 40 DAP, but no genetic parameter was significant for disease severity. This cross is considered less promising.
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