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Articles by P. Gupta
Total Records ( 2 ) for P. Gupta
  M. A. Ganie , B. A. Laway , S. Nisar , M. M. Wani , M. L. Khurana , F. Ahmad , S. Ahmed , P. Gupta , I. Ali , I. Shabir , A. Shadan , A. Ahmed and S. Tufail
  Aims  Wolfram syndrome, also known as DIDMOAD, is a relatively rare inherited neurodegenerative disorder, first evident in childhood as an association of juvenile-onset diabetes mellitus and optic atrophy, followed by diabetes insipidus and deafness. The aim of the study was to examine the clinical profile of patients with DIDMOAD syndrome presenting to a tertiary care hospital in north India.

Methods  Clinical presentation of juvenile-onset diabetes mellitus fulfilling the diagnosis of Wolfram syndrome was studied using a prepared standardized form.

Results  Subjects with juvenile-onset non-autoimmune diabetes mellitus attending the diabetic clinic at a tertiary care centre in north India were followed for 10 years and a diagnosis of fully developed Wolfram syndrome was confirmed in seven individuals. The series consisted of five male and two female patients with a mean age of 17.5 ± 7.34 years. Two subjects had consanguinity and none had any other family member affected. Optic atrophy was present in all, sensorineural hearing loss in 4/7, central diabetes insipidus in 4/7 and nephrogenic diabetes insipidus in 2/7 subjects. The new associations found were: spastic myoclonus, short stature with pancreatic malabsorption, nephrogenic diabetes insipidus, cyanotic heart disease and choledocholithiasis with cholangitis. Genetic analysis revealed mutation in exon 8 of the WFS1 gene in all the cases studied.

Conclusions  The present clinical series of Wolfram syndrome reveals a varied clinical presentation of the syndrome and some new associations.

  P. Gupta , L.S.B. Upadhyay and R. Shrivastava
  Lipase-catalyzed transesterification of vegetable oils is an important reaction that produces fatty acid alkyl esters which are valuable intermediates in oleo chemistry and are excellent substitutes to diesel fuel. In present work lipase producing bacteria were isolated from oil spilled soil samples collected from different areas of Raipur, India by serial dilution method. Lipase activity of extracellular lipase was determined by titrimetric method. The sodium alginate entrapment was carried out to immobilize lipase according to the standard method. Out of 15 bacterial isolates (LPB1-LPB15), LPB1 exhibited the maximum extracellular lipase activity on lipase assay medium. Thus, it was selected for further study. Olive oil was found to be the best substrate for lipase production (0.0070 μg/mL /min) among the substrates tested. This isolate exhibited further increase in activity with value of 0.0099 μg/mL/min using olive oil as substrate in production medium supplemented with lecithin as emulsifier at pH 7.2 after 3 days of incubation at 30°C (160 rpm). The transesterification capability of the crude extracellular lipase from LPB1 was assessed using thin layer chromatography by using hexane/diethyl ether/acetic acid as solvent system in the ratio of 90:10:1 (v/v/v). The free extracellular lipase exhibited the formation of methyl esters with the vegetable oils tested such as karanja (Rf 0.59), neem (Rf 0.59), castor (Rf 0.6) and olive oil (Rf 0.62). Both the soluble and immobilized lipase of this isolate demonstrated the methanolysis of non edible oil of Karanja (Pongamia pinnata) within 1-3 h.
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