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Articles by P. Dwivedi
Total Records ( 2 ) for P. Dwivedi
  S. Sundaram , I.Z. Ahmad and P. Dwivedi
  An efficient protocol for in vitro regeneration of endangered medicinal plant Calotropis procera has been developed. The juvenile leaf explants were transferred to MS medium containing different combinations of PGRs. Among the various combinations of Kinetin (1.0-3.0 mg L-1) and 2,4-D (1.0-5.0 mg L-1) the intensity of callus induction was highest in MS medium with Kinetin (1 mg L-1) and 2, 4-dichloro phenoxy acetic acid (3 mg L-1). The percentage of calli forming embryos was 82.6. During organogenic callus formation, different types of calli with variation in color and texture were noticed and among them, the light green, fragile calli responded well for the induction of shoots. Among the various combinations of BAP and IAA used the differentiation was seen to be the best in the presence of 2 mg L-1 BAP and 1 mg L-1 NAA frequency of shoot regeneration was highest 75%. The shoot was transferred to MS media for root regeneration containing PGRs BAP (1-4) +NAA (1-4) mg L-1. The frequency of root regeneration was 100% in MS medium containing BAP (1) + NAA (4) mg L-1. The protocol was optimized by manipulations of different PGRs for enhanced multiplication. Protocol explained in this research paper provides a rapid plant regeneration system which could be used for the somaclonal variation; shoot induction and producing transgenic plants in Calotropis.
  S. Sundaram , P. Dwivedi and S. Purwar
  Antibacterial properties of different extracts of Chlorophytum borivilianum were studied. Ethanol, ethyl acetate, acetic acid and water were used to prepare the extract. The antibacterial activity of different extracts were carried out against four bacteria, Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa and Bacillus subtilis, by agar cup diffusion method. Zone of inhibition produced by different extracts were measured. Acetic acid extract of C. borivilianum showed antibacterial activity against all the tested bacteria in the order of sensitivity as Staphylococcus aureus>Pseudomonas aeruginosa>Escherichia coli>Bacillus subtilis. The antibacterial activity of Staphylococcus aureus was sensitive with 6, 24, 12 and 8 mm zone of inhibition at 10 mg mL-1 of water, acetic acid, ethanol and acetone extract respectively. For, Pseudomonas aeruginosa zone of inhibition is 8, 20, 12 and 10 mm for water, acetic acid, ethanol and acetone. Escherichia coli revealed no zone of inhibition for water extract whereas it possess 18, 10, 2 mm zones of inhibition at 10 mg mL-1 for acetic acid, ethanol and acetone respectively. Bacillus subtilis showed 3, 20, 9 and 4 mm zone of inhibition at 10 mg mL-1 for different extracts. These results showed that the extract has a wide range of antibacterial property than the other extracts.
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