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Articles by P. Das
Total Records ( 2 ) for P. Das
  Sanjiva Bimal , Shubhankar K. Singh , P. K. Sinha , P. K. Das , H. Schallig , P. Das and S. K. Bhattacharya
  The efficacy of aqueous and freeze dried (FD) antigen was evaluated by direct agglutination test (DAT) for sero-diagnosis of visceral leishmaniasis. A total of 16 samples collected from 5 confirmed Kala-azar (KA) cases, 6 cases 2 each of filariasis, malaria, leprosy and 5 non-endemic healthy subjects were used in determination of cut off titre of both the antigens in DAT. A 1:800 for aqueous and 1:400 dilution for FD DAT antigen was found as cutoff values for differential diagnosis of KA cases from control subjects. Both antigens revealed 100% correlation in terms of sensitivity and specificity in diagnosis of KA cases and healthy subjects at these titres. Using both antigens and the cut off titres, sera from 26 individuals collected from different categories of leishmaniasis viz. Kala-azar pre-treatment 10, Kala-azar post-treatment 10, HIV-KA co-infection 1 and PKDL 5 were evaluated by DAT. Both the antigens showed equal sensitivity and specificity in diagnosis of different categories of leishmaniasis. The study concludes that though aqueous antigen showed equal sensitivity and specificity but FD antigen which is stable at 56oC do not require cold chain whereas aqueous antigen is unstable at room temperature and require proper cooling to retain its antigenic activity as studied in between day 1 and day 14, so the FD antigen should be used for routine diagnosis of KA cases particularly in field condition.
  Pranati , Sanjiva Bimal , K. Pandey , P.K. Sinha , A.K. Gupta , Shubhankar K. Singh , Shanthy Sundaram and P. Das
  Cytokine behavior of T-lymphocytes is not very clear when a patient is co-infected with Leishmania donovani and HIV. To achieve this we studied groups of patients comprising of VL infected, HIV infected and HIV-VL co-infected cases. As compared to lone infections by either of the infecting agent, the co-infection cases showed greatly diminished CD4 T-cell counts and slightly enhanced values of cytokines (IFNγ and IL4). The effect of 63 KDa antigenic fraction of L. donovani, previously reported by our group as having potential immunoprophylaxis effects, was also studied for its effect on T-cell cytokine pattern in the co-infection cases. It was interesting to find that this antigenic fraction showed an increase in the proportion of CD4 T-cells secreting IFNγ in both VL (alone) and HIV-VL co-infection groups.
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