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Articles by P. Daisy
Total Records ( 7 ) for P. Daisy
  A. Nirmala , J. Eliza , M. Rajalakshmi , Edel Priya and P. Daisy
  Medicinal plants play a major role in the management of Diabetes mellitusespecially in developing countries. The present study investigated the possible protective effects of hexane extract of Cassia fistula bark on certain biochemical parameters in Streptozotocin (STZ) induced diabetes in rats. Cassia fistula (Caesalpinaceae) has been used in traditional medicine. The barks of C. fistula have already been scientifically proved to possess anti-oxidant properties. The hypocholesterolemic and hypoglycemic effects of the hexane extract of stem bark of C. fistula, in normal and streptozotocin induced diabetic rats, were investigated in the present study. Hexane extract of C. fistula bark at doses 0.15, 0.30, 0.45 g kg-1 body weight for 30 days suppressed the elevated blood glucose levels in diabetic rats. The extract at 0.45 g kg-1 was found to be comparable with glibenclamide, the reference drug. The lipid profile (total cholesterol, triglyceride, HDL-cholesterol, LDL and VLDL-cholesterol) after the extract treatment at 0.45 g kg-1 body weight showed remarkable improvement compared to the diabetic control animals. Antioxidant and polyphenol content present in the extracts might contribute to the antihyperglycemic and antilipidemic properties. Thus the results suggest that Cassia fistula barks would be effective in the treatment of diabetes and in prevention and management of coronary artery disease.
  R. Jasmine , P. Daisy and B.N. Selvakumar
  Methicillin Resistant Staphylococcus Aureus (MRSA) has gained much attention in the last decade, as the MRSA is a major cause of hospital acquired (nosoconical infections). β-lactam antibiotics are the preferred drugs against S. aureus infections, although S. aureus has developed resistance to the β-lactam antibiotics due to the production of chromosomal or plasmid mediated β-lactamases or by producing Pencillin Binding Proteins (PBPs). The Extended Spectrum β-Lactamase (ESBL) producers are highly resistant to several conventional antibiotics. This limits therapeutic options. Hence efforts are now taken to screen few medicinal plants, which are both economic and less toxic, against the ESBL producers. Among the several plants screened, we have chosen to screen the alcohol extracts of a traditional medicinal plant, Elephantopus scaber (Asteraceae) against several clinical strains of ESBL producing MRSA. ESBL producers were screened by double disc synergy test. Methanol, hexane and acetone extracts of Elephantopus scaber were investigated for their ability to inhibit the growth of the chosen ESBL producing multidrug-resistant bacteria by the disc diffusion method. Minimal Inhibitory Concentrations (MICs) were determined by micro broth dilution method. Synergistic interaction of plant extracts with certain antibiotics was also evaluated. On the basis of promising activity, acetone extracts were fractionated and their phytochemical analysis showed the presence of terpenoids, proteins and traces of steroids. TLC bioautography of the fraction showed the active compound to be terpenoids. The strong in vitro antibacterial activity of terpenoid derivatives against ES βL-producing MRSA bacteria suggests the compounds might find wide pharmaceutical use. Further investigations to elucidate the active compound are required.
  R.C.R. Latha and P. Daisy
  Terminalia bellerica Roxb. is extensively used in Indian traditional systems of medicine to treat various diseases including diabetes mellitus. The present study investigated the antidiabetic effects of Terminalia bellerica fruit extracts and their protective effect in preventing the secondary complications of diabetes mellitus. Hexane, ethylacetate and methanol crude extracts of Terminalia bellerica fruits were administered at the dose of 200, 300 and 300 mg kg-1, respectively, for 60 days to Streptozotocin induced diabetic male Wistar rats. It was found that the fruit extracts significantly (p<0.05) increased the plasma insulin, C-peptide and glucose tolerance levels compared to the diabetic control and the effect was more pronounced in methanol extract treated rats. In addition the plant extracts significantly (p<0.05) increased body weight and serum total protein and significantly decreased the serum levels of total cholesterol, triglycerides, low density lipoprotein cholesterol, urea, uric acid and creatinine in diabetic rats. Thus the results of this experimental study indicated that Terminalia bellerica fruit extracts restored all the biochemical parameters related to the patho-biochemistry of diabetes mellitus and prevented diabetic nephropathy, dyslipidemia and other diabetes-induced complications. These beneficial therapeutic effects of Terminalia bellerica fruits may be due to the synergistic action of more than one bioactive compound and due to the significantly increased C-peptide in extract treated diabetic rats.
  P. Daisy , Nirmala A. Rayan and D. Rajathi
  The antidiabetic activity of the aqueous extract of Elephantopus scaber (Linn) root and leaf was evaluated in normal and alloxan-induced hyperglycemic rats. Oral administration of Elephantopus scaber root and leaf extracts (0.3 g kg-1 body weight) for 12 weeks resulted in significant reduction in the glucose levels. The effect of these extracts on triglycerides, HDL, cholesterol, serum urea and creatinine were also assessed to evaluate their activity in controlling diabetes related metabolic alterations. The biochemical estimations were complemented with the immunocytochemical staining to localize pancreatic islets cells secreting insulin. Though treatment with humulin (0.6 g kg-1 body weight) is more effective in normalizing blood glucose levels, regeneration of islet β-cells was mediated only by the extract treatment. The results indicate the effective role of the root and leaf extracts as an hypoglycemic agent against alloxan-induced diabetes in rats.
  R. Jasmine , P. Daisy and B.N. Selvakumar
  The extended spectrum β-lactamase producers are highly resistant to several conventional antibiotics. Hence efforts are now taken to screen few medicinal plants against the ESBL producers. Among the several plants screened, we have chosen to screen the alcohol extracts of a traditional medicinal plant, Elephantopus scaber (Asteraceae) against ESBL producers. ESBL producers were screened by double disc synergy test. Methanol, hexane and acetone extracts of Elephantopus scaber were investigated for their ability to inhibit the growth of extended spectrum β-lactamases (ESBL) producing multidrug-resistant enteric bacteria by the disc diffusion method. MICs were determined by micro broth dilution method. The crude plant extracts demonstrated zones of inhibition in the range of 5-16 mm against the chosen test bacteria. On the basis of promising activity, acetone extracts were selected to determine their efficacy in terms of Minimal Inhibitory Concentration (MIC), which ranged from 1.6-25 mg mL-1. The acetone extract was subjected to activity-guided fractionation. The most effective fraction had a MIC of 62.5-250 μg mL-1. Phytochemical analysis showed the presence of terpenoids, proteins and traces of steroids. TLC bioautography of the fraction showed the active compound to be terpenoids. The fraction was further tested for their in vivo cytotoxic activity to mammalian system using rats. No marked manifestations were observed. Normal liver and kidney functioning were also observed. The strong in vitro antibacterial activity of terpenoid derivatives against ESβL-producing Gram-negative bacteria suggests the compounds might find wide pharmaceutical use.
  P. Daisy and R. Jasmine
  In type-II diabetic individuals, there is an increase in hepatic glucose production impaired insulin signaling, decreased glucose transport and phosphorylation and diminished glycogen synthesis contribute to insulin resistance in target tissues. Liver and skeletal muscles are major target sites for insulin-mediated glucose uptake, metabolism and utilization in humans. Indeed, impaired insulin action in liver and skeletal muscles is responsible for the majority of the decreased levels of non-oxidative glucose disposal observed in type II diabetes. The influences of Elephantopus scaber (ES) (roots and leaves) on glucose oxidation in liver and skeletal muscles were studied. The present study shows that E. scaber extracts have a positive role in glucose oxidation and corrects the metabolic alterations caused by diabetes effectively. A significant reduction in the blood glucose levels and a corresponding increase in the serum insulin levels further proves the hypoglycemic activity of the plant by oxidation of glucose. These observed effects of E. scaber on glucose oxidation in liver and skeletal muscles are comparable to the effects of insulin and suggest a possible therapeutic effect of E. scaber on glucose oxidation in diabetes. Further studies on the isolation of active compounds and determining their mode of action would be of great interest.
  R. Jasmine , P. Daisy and B.N. Selvakumar
  Bioactivity-guided fractionation of the acetone extract of Elephantopus scaber (ES) yielded a new terpenoid, 6-[1-(10,13-dimethyl-4,5,8,9,10,11,12,13,14,15,16,17-dodecahydro-1H-cyclopenta[α]phenanthren-17-yl)ethyl]-3-methyl-3,6-dihydro-2 H-2-pyranone. The structure of the above mentioned compound was elucidated by interpretation of their spectroscopic data. Biological testing of the compound demonstrated significant antibacterial activity against a few multi drug-resistant ESBL-producing clinical isolates. The crude plant extracts demonstrated zones of inhibition in the range of 5-16 mm against the chosen test bacteria. On the basis of promising activity, acetone extracts were selected to determine their efficacy in terms of Minimal Inhibitory Concentration (MIC), which ranged from 1.6-25 mg mL-1. The acetone extract was subjected to activity-guided fractionation. The most effective fraction had a MIC of 62.5-250 μg mL-1. Phytochemical analysis showed the presence of terpenoids, proteins and traces of steroids. TLC bioautography of the fraction showed the active compound to be terpenoids.
 
 
 
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