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Articles by P. Chutichudet
Total Records ( 14 ) for P. Chutichudet
  B. Chutichudet , P. Chutichudet and S. Kaewsit
  An analyzing study to evaluate the relationship between Polyphenol Oxidase (PPO) activity, internal qualities and degree of browning was conducted on lettuce var. Grand Rapids in order to understand the basis changes relating to the appearance of leaf browning and some quality characteristics. For PPO activity, Factorial experiment in completely randomized design was arranged with two factors: developmental stage (Factor A) at four levels (28, 42, 59 and 73 days after planting, DAP) with three different parts of plant (Factor B) (stem, leaf and root), while Completely Randomized Design (CRD) was applied for studying the internal qualities and browning appearance in leaves at 28, 42, 59 and 73 DAP. The experiment was carried out during May-July, 2009, with four replications, ten plants per replication. The results showed that each developmental stage and the various sections of the plant affected PPO activity. The leaf section of the plant particularly affected PPO, especially at 73 DAP. In addition, at the more mature stages of the leaf, more phenolic substance, ascorbic acid content and pH value were apparent, while quinone content decreased at advancing leaf age. In addition, as the plants reached an older stage, the leaf colour in terms of L* and b* decreased. Thus, the appropriate harvest for lettuce var. Grand Rapids should be at 59 DAP in order to face fewer incidence of browning and maintain good quality.
  P. Chutichudet , B. Chutichudet and K. Boontiang
  The siam tulip is a relatively new exported cut flower that has become widely recognized for its attractive colorful pink bract. The main problem limiting this lovely flower’s value is its poor vase life after cutting. The objective of this study was to extend siam tulip’s shelf life by using a substance against ethylene action, 1-Methylcyclopropene (1-MCP). The experiment was arranged in a factorial completely randomized design, composed of two factors: 1-MCP concentration at four levels (0, 300, 600 or 900 ppb) and a period of fumigation at two levels (4 or 8 h) in a hermetically sealed plastic bucket (50 L) at 25°C. The results showed that flowers treated with 300 ppb 1-MCP for 8 h had maximal water uptake, preserved the highest anthocyanin content and the least occurrence of bract browning during postharvest life. No marked differences in weight loss of flowering stalk was observed.
  B. Chutichudet and P. Chutichudet
  A study to evaluate boron, in terms of borax (B4O.2Na.10H2O) or boric (H3BO3) by foliar spraying, on growth and external qualities was conducted on lettuce var. Grand Rapids under field conditions. A Factorial in Completely Randomized Design was arranged with four replications and composed of two factors; two types of boron (borax or boric) with four concentration rates (0, 0.0625, 0.125 or 0.1875%). The results showed that plants-treated with 0.0625% boric had the maximal plant height and bush size. While two types of boron at any concentration had no effect to biomass, chlorophyll content and the leaf colour. Furthermore, plants treated with 0.0625% boric experienced the lowest browning appearance at harvesting stage.
  Benjawan Chutichude , P. Chutichudet and S. Kaewsit
  The purpose of this experiment was to investigate the effect of preharvest soil application of dolomite on the growth, activity of Polyphenol oxidase (PPO) and internal characteristics was evaluated on lettuce cv. Grand Rapids under field conditions. A factorial in completely randomized design was arranged with four replications and composed of two factors; application time four levels (25, 40, 55 days after planting, DAP compared with untreated treatment, Control) with four concentration rates (0, 50, 100 and 150 ppm). The results show that dolomite application irrespective of application times or concentration rates had no effect on stem diameter, plant height, degree of leaf browning, fresh weight, biomass, chlorophyll content, leaf colour in terms of a* and b*, the content of phenolic, quinone, Total Soluble Solids (TSS), Titratable Acidity (TA), pH or ascorbic acid content. While maximum response of leaf increment was achieved with treating of 150 ppm dolomite at 25 DAP. Dolomite application irrespective of concentrations at all application times (25, 40 and 55 DAP) reduced the bush size compared with the control. In addition, application of 150 ppm dolomite at 55 DAP had the maximal brightness of leaf colour, L* value. Furthermore, dolomite treatment of 50 ppm at 25 DAP gave the least level of PPO activity at 33 DAP.
  P. Chutichudet , B. Chutichudet and K. Boontiang
  Patumma is one of the most popular exported cut flowers in Thailand due to its attractive large pink bracts. Its export value, however is limited because of its poor vase life. The objective of this research was to extend patumma’s shelf life by using a substance against ethylene action, 1-Methylcyclopropene (1-MCP). The experiment was arranged in a Factorial in Completely Randomized Design, composed of two factors : 1-MCP concentration at three levels (0, 100 and 300 ppb) with period of fumigation at two levels (12 and 15 h) in a hermetically sealed plastic bucket (50 L) at 20°C. Afterwards, the treated and untreated flower stems were dipped in a plastic bottle filled with distilled water and stored in ambient temperature (27°C, 91% RH). Weight loss of flowering stalk, water uptake by flowering stem, vase life, anthocyanin content and bract colour were recorded every other day at ambient temperature. The results showed that flowers treated with 300 ppb of 1-MCP for 15 h had the least weight loss of the flowering stem and preserved the highest anthocyanin content at 8 DAV. For water uptake by the flowering stem, flowers treated with 100 ppb of 1-MCP for 12 h gave the highest water uptake from six to ten days after vase life (DAV), while the maximal vase life (10.25 days) of flowers treated with 100 ppb of 1-MCP for 12 h was observed. For bract colour, the results showed that treatment with 100 ppb of 1-MCP for 15 h gave the maximal L* and a* values at 12 DAV.
  Chomdao Khumjing , B. Chutichudet , P. Chutichudet and K. Boontiang
  This study was aimed at evaluating the effect of different calcium formulas with various concentration rates on leaf color, contents of phenolic compounds and quinone, Polyphenol oxidase (PPO) activity and browning percentage in Grand Rapids lettuce. A Factorial in Completely Randomized Design was arranged with four replications and composed of two factors: three forms of calcium in terms of calcium chloride (CaCl2), calcium nitrate [(Ca(NO3)2] or calcium oxide (CaO) by soil dressing application with five concentrations (0, 0.5, 1.0, 1.5 or 2.0%). The results showed that plants-treated with 1.5% CaCl2 had the maximal leaf color in term of greenness (a*) values, while treatment of 2% CaCl2 had the lowest contents of phenolic compounds, quinone and activity of Polyphenol oxidase (PPO) which corresponded to the least browning level at harvesting stage.
  Benjawan Chutichudet and P. Chutichudet
  The effects of preharvest different shading (0, 50, 60, 70 and 80%) on the contents of phenolic and quinone, leaf color, browning appearance and activity of Polyphenol Oxidase (PPO) were evaluated on lettuce cv. Grand Rapids under field conditions. A Completely Randomized Design was arranged with four replications and composed of five treatments: no shading (Control) compared with shading sunlight to 50, 60, 70 or 80% when the plants aged 15 Days After Planting (DAP). The results showed that plants grown under shading of 60% sunlight had the lowest contents of both phenolic and quinone substances. In addition, leaf color from plants treated with 60% shading showed the lowest a* value. Furthermore, at harvest, both the least browning damage and the minimal activity of PPO were achieved with the treatment of 60% reduction of sunlight.
  B. Chutichudet and P. Chutichudet
  This study aimed to evaluate the effect of two calcium formulas (CaCl2 and CaH4O8P2) at 0.4 and 0.8% on physical and chemical changes to Chiang Mai Pink patumma. The evaluation which included a comparison with control, included an assessment of weight loss, water absorption, bract color, polyphenol oxidase (PPO) activity, phenolic content, browning incidence and vase life. A completely randomized design was arranged with five treatments: CaCl2 0.4%, CaCl2 0.8%, CaH4O8P2 0.4%, CaH4O8P2 0.8% compared with control. The experiment was carried from May to July 2010 with four replications and ten flowers per replication. The results showed that treating with both calcium solutions (CaH4O8P2 0.4%, CaH4O8P2 0.8%) led the flowering stem to absorb less water. At 0.8% CaH4O8P2 caused maximal bract color in terms of a* and b*, highest phenolic content and browning severity. These above characteristics brought about the shortest vase life (7.60 days). The Control flower was found to have minimum PPO activity, phenolic substance and browning damage. Thus, applications of CaCl2 and CaH4O8P2 at 0.4 and 0.8% were found to be not the appropriate substances for maintaining the quality and prolonging the vase life of the patumma’s flower.
  Benjawan Chutichudet and P. Chutichudet
  Patumma flower is one of the new exported cut flower that faces with the poor vase life affecting to this flower’s price after cutting. Effects of true flower thinning in conjunction with 1-MCP fumigation at 0, 100 and 300 ppb on postharvest changes and vase life of patumma flower 'Chiang Mai Pink' was studied at ambient conditions (27°C, 91% RH). A Factorial in Completely Randomized Design was arranged with two factors: flower thinning and 1-MCP concentrations. The experiment was conducted from May-September 2011 with four replications and ten flowers per replication. Flowering weight loss, water uptake, bract color and vase life were recorded every other day. The results showed that treatments with true flower thinning, irrespective of 1-MCP application, caused the least flowering weight loss and maintained the highest bract color in terms of L* and a*. In addition, deflowered flowers with no treatments with 1-MCP had maximal vase life of 20.10 days.
  P. Chutichudet , Benjawan Chutichudet and S. Kaewsit
  This laboratory experiment was carried out at the Department of Agricultural Technology, Mahasarakham University, Northeast Thailand during June to October 2007. The experiment aimed to search for the most appropriate harvesting age of fruits of Santol orchard plants with respect to colour, tissue texture, total soluble solid content, titratable acidity and pH of Santol fruits. A Randomised Complete Block Design (RCBD) with four replications was used. Each replication consisted of 10 fruits, thus a total of 160 fruits were used. The Santol fruits were harvested at different ages, i.e., 100, 115, 130 and 145 days after full bloom of flowers and these harvested ages were used as treatments, i.e., 100 for T1 (Control), 115 for T2, 130 for T3 and 145 for T4. The results showed that the most appropriate harvesting date for high quality Santol fruits was found with T4, i.e., 145 days after full bloom of flowers where Santol fruits of T4 gave the highest mean values of fruit length, diameter, fresh weight fruit-1 of 10.71 cm, 9.31 cm and 399.76 g, respectively. Yellowness of skin colour of fruits was evenly distributed. Total soluble solid content of pericarp, flesh tissue and seeds were highest for T4 with mean values of 13.93, 15.05 and 18.260 brix, respectively. Flesh texture density highly decreased with an increase in numbers of days after full bloom of flowers. Titratable acidity content in fruits was highly decreased with an increase in numbers of days after full bloom of flowers whereas a reverse was found with pH of fruit juices.
  Chutichudet Benjawan , P. Chutichudet and T. Chanaboon
  This investigation was carried out at Mahasarakham University Experimental Farm, Mahasarakham University, Northeast Thailand in the late rainy season of the 2003 to 2004 with the use of Roi-Et soil series (Oxic Paleustults). The experiment aims to search for more information on the effect of different rates of chemical Paclobutrazol (PBZ) application on growth, yield and quality of edible okra pods. A Randomised Complete Block Design (RCDB) with four replications was used for the experiment. The experiments consisted of five treatments, i.e., 0 (T1), 4,000 (T2), 8,000 (T3), 12,000 (T4) and 16,000 ppm ha-1 (T5) of chemical PBZ. The results showed that an increase in PBZ application rate highly decreased plant height, harvesting age and significantly decreased leaf area of the fifth leaf but highly increased pod length, fresh weight/pod and fresh pod yield ha-1 of the okra plants. PBZ had no significant effect on stem diameter and diameter of pods of the okra plants. Total soluble solid, fibre content, titratable acid, vitamin C and pectin contents in pods were not affected by chemical PBZ application. Pod yield highly increased with an increase in rate of PBZ application. The highest edible pod yield reached a value of 4,501 kg ha-1 for the highest rate of PBZ application (T5).
  Chutichudet Benjawan , P. Chutichudet and S. Kaewsit
  Abstract: This green manure experiment with the use of okra crop as indicator plant was carried out at Mahasarakham University Experimental Farm, Mahasarakham province, Northeast Thailand during May to September 2005 to investigate four types of green manure legume crops on growth, yield and quality of edible fresh pods of okra crop when grown on Roi-Et soil series (Oxic Paleustults). The four types of green manure plants include Jack bean, Cowpea, Green gram, and Giant sensitive plant. The experiment consisted of five treatments, i.e., T1 (Control), T2 (Jack bean), T3 (Cowpea), T4 (Green gram) and T5 (Giant sensitive plant). The experiment was laid in a Completely Randomized Design (CRD) with four replications. The results showed that Roi-Et soil series (Oxic Paleustults) contained some considerable mean values of organic matter (1.64-1.66%) but soil available phosphorus and exchangeable potassium were relatively low, particularly potassium. Green manures of the four legume plants slightly improved soil property of the Roi-Et soil series (tested at the end of the experimental period). The most effective green manure on stem diameter, bushy diameter, leaf numbers plant- 1 and leaf area of the fifth leaf of the okra plants, in most cases, was found with Jack bean and Cowpea ranked the second. However, in most cases, Cowpea gave a similar effect as that of Green gram and Giant sensitive plant. Pod length and weight pod- 1, pod diameter and edible fresh pod yields (5,941.86 kg ha- 1) were highest with Jack bean green manure treatment (T2), whilst the rest, in most cases, were similar. Green manure treatments gave highly significant effect on total soluble solids of pods over the control treatment, whilst total acidity, fibre and pectin contents were unaffected by green manure treatments. Green manure of Jack bean was the best legume crop for green manure to be used in improving soil fertility, particularly for Roi-Et soil series (Oxic Paleustults).
  Chutichudet Benjawan and P. Chutichudet
  This laboratory experiment was carried out at the Department of Agricultural Technology, Mahasarakham University, Northeast Thailand during July and August 2008. The experiment aimed to determine an effective natural organic acid that would delay the unattractive skin browning of santol fruit, while at the same time not damaging the quality of the fruit. The experiment included a study of the fruit’s polyphenol oxidase (PPO) activity, phenolic content and quinone content, as they relate to colour and a study of total soluble solid content, pH, titratable acidity and vitamin C content as they relate to fruit quality. A Completely Randomized Design (CRD) with four replications was used. Each replication consisted of 10 fruits. Santol fruit was harvested at 145 days after full bloom and dipped for 30 min in aqueous solutions of two organic acids that were used as treatments, i.e., 0% for T1 (control), 5% citric acid for T2, 5% ascorbic acid for T3, 10% citric acid for T4 and 10% ascorbic acid for T5 and stored at room temperature (28°C, 90% R.H.) to investigate the effect of the acid on fruit weight, skin colour, PPO activity and other internal parameters. The results showed that the most appropriate anti-browning agent for santol fruit was found with T2. It gave the highest mean values, 57.37 and 55.95, of brightness (L*) at 4 and 10 Days After Storage (DAS), respectively. In addition, PPO activity of flesh tissue was lowest for T2 with mean values of 0.0078 to 0.0092 by 0 and 300 S, respectively. The phenolic content in the flesh tissue significantly increased with an increase in numbers of DAS, whereas the reverse was found with the pH level in the fruits. They were lowest for T2, with mean values of 6.00, by 10 DAS. There were no significant differences among the treatments in any of the measured Total Soluble Solids (TSS), Titratable Acidity (TA) and vitamin C content.
  P. Chutichudet , B. Chutichudet and K. Boontiang
  The objective of this study was to extend patummas shelf life by using a substance against ethylene action, 1-Methylcyclopropene (1-MCP). The experiment was arranged in a Factorial in completely randomized design, composed of two factors : 1-MCP concentration at four levels (0, 300, 600 or 900 ppb) and period of fumigation at two levels (4 or 8 h) in a hermetically sealed plastic bucket (50 L) at 25°C. Afterwards, the treated and untreated flower stems were dipped in a plastic bottle filled with distilled water and stored in ambient temperature (27.5°C, 91% R.H.). Each treatment consisted of 10 replicates, one flower per replication. The experiment was carried out from June to August, 2008 at the laboratory of the Division of Agricultural Technology, Faculty of Technology, Mahasarakham University, in the Northeast of Thailand. The results showed no obvious differences in shelf life, except that the flower-treated with 600 ppb of 1-MCP for 8 h had the least storage life of 6.40 days. Treatment with 1-MCP had no effect on water uptake, rot appearance and wilting percentage. In addition, flower-treated with 1-MCP at 600 and 900 ppb for 4 and 8 h retained a higher content of anthocyanin by 26.57 and 16.32 mg/100 g Fresh Weight (FW) by 4 and 10 days after vase life (DAV), respectively.
 
 
 
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