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Articles by P. Chowdhury
Total Records ( 2 ) for P. Chowdhury
  P. Chowdhury , R. Hari , B. Chakraborty , B. Mandal , S. Naskar and Nirmalendu Das
  Pleurotus fossulatus (Cooke) Sace is member of oyster mushroom can produced extracellular laccase (benzenediol: oxygen oxidoreductase; EC 1.10.3.2) in submerged fermentation. To analyze the optimum production for laccase P. fossulatus was cultured both in stationary and shaking condition in different media. Partial purification of laccase was done after 0-80% ammonium sulphate precipitation, followed by DEAE (Diethylaminoethyl) Sephadex (A-50) anion exchange chromatography. Potato-sucrose peptone (PSP) medium and Potato-dextrose (PD) medium showed highest laccase production in shaking and stationary conditions, respectively. Though the time required for optimum laccase production in stationary condition was much more than the shaking condition but the amount of laccase was about 2.75t greater in former condition. The laccase produced in stationary condition was more stable than the enzyme produced in shaking condition. The partially purified enzyme showed highest affinity towards o-dianisidine than guaiacol and ABTS (2,2’- Azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) as evidenced by their Km. The physico-chemical properties of the laccase suggested the significance of this enzyme in industrial applications.
  N. Das , S. Naskar , P. Chowdhury , B. Pasman and D. Adhikari
  The aim of the present study was to find out a common laccase isoenzyme in five Pleurotus spp. which might be responsible for regulation of mycelial growth in producer organisms. Laccase activities were assayed from the extracellular culture filtrates and optimum dates for laccase production under stationary condition were detected. Partially purified 25th days culture filtrates were analyzed for activity staining in 10% Native PAGE. The culture filtrate of all the strains had shown the oxidization of o-dianisidine, guaiacol and ABTS with different efficiencies. P. ostreatus showed optimum laccase activity in 25th day whereas other strains shown optimum activities in 26th day. The highest laccase activity and biomass were found in P. ostreatus and lowest in P. florida. Though different Pleurotus species exhibited differential laccase isoenzyme expression patterns but one isoenzyme band had been found to be common in all the species, which resulted in similar rf value with that of a previously reported growth regulating laccase (L2) of P. florida.
 
 
 
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