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Articles by P. Lakshmanaperumalsamy
Total Records ( 4 ) for P. Lakshmanaperumalsamy
  R. Subashkumar , Tha. Thayumanavan , C. Thilagavathy G. Vivekanandhan , K. Savithamani and P. Lakshmanaperumalsamy
  A total of 105 milk samples were analysed for Aeromonas hydrophila and 17.14% of the milk samples were found to be contaminated with A. hydrophila. Out of the milk samples, 30.5% were found to be of poor quality. The A. hydrophila strains were resistant to bacitracin, cefazolin and novobiocin and sensitive to gentamycin, kanamycin, neomycin and polymyxin-B. About 94.4% of strains were found to be haemolysin producers. Proteolytic activity was exhibited by all the isolates. The environmental factors such as NaCl and pH were influencing the growth of the A. hydrophila isolates. None of the RAPD and ERIC profiles were found identical. This clearly revealed the wide genetic heterogeneity among the strains tested.
  A. Vijaya Chitra and P. Lakshmanaperumalsamy
  The potential of the bacterial isolates to biodegrade nitrate from explosive industry effluent was tested using a batch scale process. Three bacterial species capable of biodegrading nitrate were isolated from effluent and sludge samples taken from a washwater soakaway manufacturing nitroglycerin and slurry explosives. Bacteriological analysis of the samples revealed the presence of about 58% nitrate reducing bacteria belonging to the genera Alcaligenes, Corynebacterium, Bacillus, Pseudomonas and Micrococcus. Among the isolates Pseudomonas sp.-NGS 5, Bacillus sp. NGS 6 and Corynebacterium sp.-SEE 12 were found to be efficient in nitrate reduction. Physico-chemical parameters were also analyzed for all the samples. Individual and different bacterial consortia were used for the removal of nitrate in synthetic solution. It was found that the bacterial consortium was efficient in nitrate removal in the effluent. The consortium combination-Pseudomonas, Bacillus and Corynebacterium was used for the removal of nitrate in nitroglycerine and slurry explosive effluents that were diluted to different concentrations viz., 25, 50, 75 and 100%. The percentage of nitrate removal by the bacterial consortium was 55 and 58% in nitroglycerine and slurry explosive effluent, respectively.
  R. Subashkumar , Tha. Thayumanavan , G. Vivekanandhan and P. Lakshmanaperumalsamy
  Of the samples examined, 25.7% of children diarrhoeal samples harbored Aeromonas spp. Among them 16.7% were A. hydrophila. Though all the isolates were resistant to all β-lactam antibiotics (except carbapenem) and non β-lactam antibiotics tested and further studied for the presence of plasmid in all the strains. The majority of isolates were resistant to clarithromycin and vancomycin. Thirty one (77.5%) of the 40 isolates harbored plasmids, with sizes ranging from 2.6 to 15.2 kb. These results indicate that multiple antibiotic resistant and genetically diverse aeromonads are easily available among the children diarrhoeal samples collected from the study area. It indicates that the practice of frequent application of therapeutics for the treatment of diseases might have enhanced occurrence of drug resistance and carrying the resistant plasmids.
  S. Rajakumar , P. Velmurugan , K. Shanthi , P.M. Ayyasamy and P. Lakshmanaperumalsamy
  The present study deals with enumeration and seasonal distribution of total and faecal coliform bacteria, the indicators of microorganisms causing faecal pollution of two lakes namely Kodaikanal and Yercaud lake, Tamilnadu, India was investigated. Water samples from 4 different sites from the lakes were collected during February 2003 to January 2004 and were analysed for total coliform count, faecal coliform count and faecal streptococci, which is an indicator organism of faecal contamination. The range of MPN 100 mL-1 for total coliform was found 46-50, 46-60 and 33-40, in Kodaikanal lake water and 94-140, 110-180 and 79-110 in Yercaud lake water in summer, monsoon and winter, respectively. In Kodaikanal lake the faecal coliform was in the range of 27-33 MPN 100 mL-1, 33-46 MPN 100 mL-1 and 17-27 MPN 100 mL-1 and in Yercaud lake it was 63-94 MPN 100 mL-1, 70-110 MPN 100 mL-1 and 63-79 MPN 100 mL-1 in summer, monsoon and winter, respectively. The range of faecal streptococci found in Kodaikanal lake water was 7-14 MPN 100 mL-1 during summer, 8-17 MPN 100 mL-1 during monsoon and 5-14 MPN 100 mL-1 during winter and that of Yercaud lake water was 17-46 MPN 100 mL-1, 26-49 MPN 100 mL-1 and 9-43 MPN 100 mL-1 in summer, monsoon and winter respectively. Bacteriological analysis of the lake waters indicated that water was polluted by faecal contaminants to the extent that they were unpotable for drinking purposes and unsuitable for recreational activity. Hence the lakes needed thorough impoundment.
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