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Articles by Ooi Wai Ling
Total Records ( 3 ) for Ooi Wai Ling
  Ooi Wai Ling , Son Radu , Gulam Rusul , Mohamed Ismail Abdul Karim , Endang Purwati and Samuel Lihan
  A total of 30 strains of Escherichia coli O157:H7 isolated from beef and chicken burger were characterized by Enterobacterial Repetitive Intragenic Consensus (ERIC) genotyping. The ERIC polymorphism patterns obtained as illustrated in a dendrogram showed a significant discriminatory fingerprint among the 30 E. coli O157:H7 strains. Nearly every isolates had a unique fingerprint and that there were no bands that were highly conserved among the isolates. This study suggests that there is considerable genetic heterogeneity among the E. coli O157:H7 strains by ERIC PCR, and that this has application in screening strains from clinical or food samples to detect a virulent strain with a known fingerprint, and to trace its dissemination.
  Son Radu , Rozila Alias , Gulam Rusul , Samuel Lihan and Ooi Wai Ling
  A total of 70 isolates of Escherichia coli O157:H7 isolated from beef samples were examined with respect to plasmid profiles and random amplified polymorphic DNA (RAPD) patterns. All isolates carried the 90 kb pO157 plasmid alone or in combination with other smaller plasmids. Using Gen1-50-02 (5’-CAATGCGTCT-3’), Gen1-50-09 (5’-AGAGGCGATG-3’) and Gen1-50-10 (5’-CCATTTACGC-3’) as primers, respectively, we obtained DNA polymorphisms which allowed us to discriminate the E. coli O157:H7 isolates into one, six and five RAPD patterns; providing bands ranging in size from 0.25 to 4.0 kb. Our results demonstrate that both plasmid profiling and RAPD-PCR fingerprinting methods are suitable tools for a fast and reliable molecular typing of E. coli O157:H7. The RAPD-PCR method is more sensitive with respect to the individualization of isolates and that RAPD-PCR assay could be a valuable technique for epidemiological studies.
  Yuherman , Son Radu , Gulam Rusul , Lum Keang Yeang , Ooi Wai Ling and Jamal Khair
  DNA fingerprinting by PCR amplification of enterobacterial repetitive intergenic consensus (ERIC) and pulsed-field gel electrophoresis (PFGE) were used to compare environmental and clinical isolates of Vibrio cholerae O1 and non-O1. All the V. cholerae O1 and non-O1 isolates were typable using ERIC PCR. Though PFGE generated banding patterns to discriminate the isolates into twelve fingerprints, eight isolates were untypable by PFGE due to consistent degradation of the bacterial DNA. Based on the dendrogram generated from ERIC-PCR method, three of the clinical isolates (C1, C2 and C3) were closely related to environmental isolates (E6 or E10). The results indicate that ERIC-PCR is a very discriminative and efficient method for studying genetic diversity of V. cholerae isolates.
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