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Articles by Olga Koutita
Total Records ( 2 ) for Olga Koutita
  Olga Koutita , Konstantinos Tertivanidis , Theodoros Koutsos , M. Koutsika-Sotiriou and George Skaracis
  Genetic diversity during selection process (starting material, material after 3 and 5 cycles of mass-selection and breeding lines produced by selfing and 4 cycles of pedigree selection) obtained from two local Greek cabbage Open-Pollinated (OP) populations was investigated, using RAPD (Random Amplified Polymorphic DNA) markers, on 12 individual plants selected from each population. A total number of 24 random primers were employed and in the entire material studied 277 bands were detected with an average of 11.54 bands/primer. Some of the bands (80 or 72 out of all the bands) revealed polymorphism in selection products of the cabbage populations at an average of 3.47 and 3.00 polymorphic bands/primer for each population. Nei`s standard genetic distances between the selected products were grouped by the Unweighted Pair Group Method and Arithmetic Average (UPGMA) clustering method. Average genetic diversity within and between the selection cycles and S-lines was evaluated. It was concluded that RAPD markers could be used to monitor changes occurring during the breeding process in the two cabbage populations.
  Konstantinos Tertivanidis , Olga Koutita , Ioannis I. Papadopoulos , Ioannis S. Tokatlidis , Efstathios G. Tamoutsidis , Vasiliki Pappa-Michailidou and Metaxia Koutsika-Sotiriou
  Genetic diversity in 19 local dry bean populations (Phaseolus vulgaris L.) originated from the region of Macedonia (Greece, GR and Formal Yugoslavic Republic of Macedonia, FYROM) was investigated using RAPD (Random Amplified Polymorphic DNA) markers in 10 individual plants from each population. A total of 11 random primers detected 56 polymorphic bands, with an average of 5.1 polymorphic bands/primer. The individual plants were grouped in 14 clusters on the basis of the Jaccard coefficient (Unweighted Pair Group Method and Arithmetic Average-UPGMA). The average taxonomic distances between the 19 populations were calculated on the basis of gene frequencies. These distances were used for grouping the populations, by UPGMA and Principal Coordinates Analysis (PCO), resulting in four and 10 groups, respectively. In general, there was a noticeable similarity in the grouping of the individuals and populations with the two methods. According the UPGMA and PCO procedures, the populations were clustered based on geographical origin. It was concluded that RAPD markers could be exploited as alternative or supplementary tools to already established methods for the evaluation and classification of bean genetic resources.
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