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Articles by Olarewaju M. Oluba
Total Records ( 3 ) for Olarewaju M. Oluba
  Olarewaju M. Oluba , Chimelu Okongwu , Temitayo Lawa and Oghenerobor B. Akpor
  Background and Objective: At present, Fish Meal (FM) and meat meal are the dominant animal protein sources for livestock feeds. However, these protein sources are not affordable for most livestock farmers, especially those in developing countries. Thus, it would be helpful to test other protein-based animal byproducts as alternative cheaper protein sources and as a solution to the overdependence of animal feeds on FM. The present study was undertaken to investigate the feasibility of replacing fish meal (FM) with chicken feather protein hydrolysate (CFPH) in rat diet. Materials and Methods: The changes in growth performance (after 4 weeks) and some tissue biochemical indices (after 7 weeks) were determined following feeding with iso-proteic and iso-energetic diets, in which 0 (control), 20, 40, 60, 80 and 100% of FM were replaced by CFPH (CFPH 0-100), respectively. Results: Food intake and weight gain showed progressive reduction with increasing proportion of CFPH in the diet increased. The growth parameters monitored were observed to decrease progressively with the increasing CFPH level in the diet. Plasma lipid concentrations were not significantly altered in the rats fed with 20 and 40% CFPH but were significantly lower in the rats fed with 60-100% CFPH compared to the rats fed with 100% FM. Fecal nitrogen excretion and plasma total protein concentration were significantly increased in the rats fed with 40-100% CFPH compared to rats fed 20 and 40% CFPH as well as 100% FM. Liver and kidney function indices monitored were not significantly altered in the rats fed CFPH up to 40% compared to the rats fed 100% FM. Conclusion: Based on the data generated from this study, it could be concluded that the inclusion of CFPH in rat diet up to 20% of the total dietary protein content is safe and has no drastic effect on the growth performance. Thus, feeding CFPH to animals could be a cost-effective solution to the challenge of waste feather disposal and could reduce the overdependence of livestock feed on FM, thereby ensuring sustainable livestock farming.
  Oghenerobor B. Akpor , Olarewaju M. Oluba , Olayinka O. Alabi , Abigail G. Adeyonu and Ayoola J. Shoyomb
  To reduce the production cost of growth medium for microbial cultivation, a variety of natural products such as milk, animal tissues and plants have been exploited. The present study was aimed at generating Chicken Feather Protein Hydrolysate (CFPH) from alkaline hydrolysis of chicken feathers as a medium component in the cultivation of selected bacteria and yeasts. Alkaline hydrolysis of the raw chicken feather was carried out to obtain CFPH. The study was carried out using different combinations of CFPH: peptone ratios for the growth medium. Three bacteria Esherichia coli, Staphylococcus aureus and Klebsiella pneumonia) and two yeasts (Candida carpophila and Candida tropicalis) species were as the test microbial species used as test microorganisms. For growth rate studies, 0.5 mL of the broth cultures of the respective test isolates was inoculated in the growth medium. Immediately after inoculation and at every 2 h interval, for a 10 h duration, aliquot samples of the inoculated broth cultures were aseptically withdrawn from each flask for optical density measurement. The study revealed 60 % CFPH yield with the hydrolysis method employed. The results obtained demonstrated that growth performance of the test microorganisms varied for each of the CFPH: peptone combinations. Generally, growth rates of the yeast were observed to be significantly higher (p<0.05) in media with CFPH: peptone combinations of 4:6, 6:4 and 8:2 than media with peptone only. Although, in most of the bacteria species investigated, growth was better in the peptone only media, media containing 4:6, 6:4 and 8:2 CFPH: peptone combinations compared well in terms of growth rate. Data obtained in this study showed the potential of CFPH as an alternative growth substrate to peptone in microbial culturing. Thus, revealing the possibility of conversion of chicken feather waste to more valuable use.
  Oghenerobor B. Akpor and Olarewaju M. Oluba
  Background and Objective: Accomplishing fast and uniform seedling development is strategic for crop vigour because delayed germination regularly open seedlings to unfavorable ecological conditions and soil-borne diseases. This study was aimed at assessing the germinability enhancement and protective potentials of leaf decoctions of four selected plants (Chromolaena odorata, CO; Nauclea latifolia, NL, Ipomoea asavifolia, IP and Moringa oleifera, MO) in comparison to hydrogen peroxide (HP), normal saline suspended cells of Pseudomonas aeruginosa (PA) and water (control) against selected fungal pathogens (Aspergillus fumigatus, Aspergillus flavus, Fusarium and Aspergillus niger) on Abelmoschus esculentus (okra). Materials and Methods: Surface sterilized seeds of Abelmoschus esculentus were soaked in the fungal pathogens for 1 h before being steeped in a given concentration of the respective decoctions for another 1 h and then planted on wet blotter for 7 days while monitoring the germination parameters. Results: The highest germination rates of 64.23 and 64.29% were observed when the seeds were primed with 5-fold dilution of CO and IP, respectively. However, germination was highest (64.28%) at 2-fold and 5-fold dilutions when primed with MO while HP-primed seed gave the germination rate was 92.86%. For seeds primed with the PA, germination rate of 71.42% was observed in the undiluted and 5-fold dilutions. The optimum soaking time was observed to be between 3 and 6 h, when the seeds were primed with the different decoctions. Following pre-treatment of the seeds with the fungal pathogens prior to priming with the different osmotica, germination rate reduced to 50%. This is significant compared to no growth observed in infected seeds without priming. Conclusion: Priming with decoctions of CO, IP and MO as well as HP and PA significantly improve germinability and confer protection against fungal infection. Thus, scale-up to field trials using CO, IP and MO decoctions, as well as HP and PA as bio-fungicide prior to planting for protection of okra seeds, seems justified as a sustainable alternative to the use of chemical fungicides.
 
 
 
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