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Articles by O.M. David
Total Records ( 2 ) for O.M. David
  O. Famurewa and O.M. David
  Avocado pear (Peasea amaricana Cmill) has an excellent nutritional quality that can support the growth of microorganisms. Different media were formulated from both defatted and undefatted dehydrated avocado pear. The proximate analyses of the pear flour show that defatted samples were better in term of minerals contents than their corresponding undefatted samples. Pseudomonas aeruginosa, Staphylococcus aureus and Escherichia coli in that order thrived very well in the composed media. The test bacteria grew better in media composed with defatted pear than their corresponding undefatted samples. Undefatted samples seem to support fungal growth than defatted samples. Trichoderma sp. grew better than Aspergillus flavus and Penicillium notatum. Comparing with the performance of conventional bacteriological and mycological media, avocado pear is a good and cheap media material for the cultivation and isolation of both bacteria and fungi.
  O.M. David , A.O. Oluduro , Shitttu , O.A. Olowe and O. Famurewa
  Haematological, enzymatic and histopathologic changes during gelatinase positive (gel+) Enterococcus feacalis infection was assessed in an animal (albino rat) model using standard methods. The role of gelatinase in post-enterococcaemia was established. White Blood Cell (WBC) count, Packed Cell Volume (PCV) and platelets were significantly reduced (at p≥0.05) in gelatinase positive (gel+) than in gelatinase-negagive (gel¯) compared to the controls. The enzymes Aspartate aminotransferase (AST), Alanine aminotransferase (ALT), Acid Phosphatase (ACP) and Alkaline Phosphatase (ALP) showed the following values 105, 43, 39.39 and 102.63 IU L-1, respectively for the gel+ infected animas, 108, 57, 164.6 and 428.94 IU L-1, respectively for gel¯ and 108, 67, 77.77 and 202.63 IU L-1, respectively for the control. The results obtained for the bilirubin test were 18.5 mg dL-1, total bilirubin and 7.83 mg dL-1 conjugated bilirubin for gel+ infected animas, total and conjugated bilirubin recorded 7.4 and 2.46 mg dL-1, respectively in gel¯ infected animas and 5.55 and 4.92 mg dL-1, respectively in the control. Histopathological changes within the individual groups varied and overall changes were less extensive than observed in animals infected with gel+ E. faecalis. Thin section showed an overall loss of structural integrity. The results show areas of pronounced haemorrhage, necrosis with bacterial clusters and distortion in morphology. There was a striking difference in the severity of lesions between gel¯ and gel+ infected animal. However, in an intraperitoneal rat infection model, gel+ strain was relatively less pathogenic. These findings highlight the importance of gelatinase as a pathogenic factor and are likely key determinants important to pathogenesis of pathogens.
 
 
 
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