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Articles by O.G. Gbenle
Total Records ( 3 ) for O.G. Gbenle
  M.N. Igwo-Ezikpe , O.G. Gbenle , M.O. Ilori , J. Okpuzor and A.A. Osuntoki
  Bacteria isolated from various contaminated soils in Nigeria were investigated for their potential to utilize and biodegrade high molecular weight polycyclic aromatic hydrocarbons which include chrysene, fluoranthene and pyrene. Biochemical and morphological studies identified the isolates as Sphingomonas sp., Pseudomonas sp. and Pseudomonas putida. Biodegradation studies showed that Sphingomonas sp., Pseudomonas sp. and P. putida degraded 100 mg L-1 chrysene to 30.5±0.3, 40.6±0.7 and 17.2±0.2 mg L-1, respectively after 8 days of incubation. Similarly, fluoranthene was degraded to 2.0±0.1, 2.0±0.4 and 0.12±0.1 mg L-1 while pyrene to 0.16±0.2, 6.5±0.3 and 6.6±0.4 mg L-1 correspondingly. Consortium of the isolates degraded 100 mg L-1 chrysene, fluoranthene and pyrene, respectively to 21.3±0.9, 2.2±0.8 and 10.6±0.8 mg L-1. In the presence of phenanthrene as co-substrate, chrysene, fluoranthene and pyrene were, respectively degraded by consortium to 12.4±0.5, 0.2±0.3 and 0.7±0.2 mg L-1 while phenanthrene was undetectable. This study showed that there was delayed degradation of chrysene and fluoranthene in the presence of phenanthrene, this may account for the persistence of these compounds in polycyclic aromatic hydrocarbons polluted sites. This is the first report on the potential of these isolates simultaneous utilization and biodegradation of chrysene, fluoranthene and pyrene when used as sole carbon and energy source.
  M.N. Igwo-Ezikpe , O.G. Gbenle , M.O. Ilori , J. Okpuzor and A.A. Osuntoki
  The experiment was conducted to evaluate the potential of tropical bacterial isolates and its consortium to biodegrade mixture of high molecular weight polycyclic aromatic hydrocarbons (chrysene, fluoranthene and pyrene). The effect of phenanthrene in the degradation was also investigated. The bacterial consortium was made up of Sphingomonas sp., Pseudomonas sp. and P. putida and biodegradation set up for 8 days with initial 100 mg L-1 substrate concentration. Degradation by Sphingomonas sp., Pseudomonas sp. and P. putida, respectively after 8 days gave higher residual chrysene of 40.2±1.4, 40.3±2.2 and 27.4±1.8 mg L-1, fluoranthene of 32.5±1.3, 35.4±1.2 and 10.1±2.5 mg L-1 and pyrene of 37.5±1.2, 34.2±2.4 and 32.0±1.2 mg L-1 compared to 11.5±1.4 (chrysene), 6.2±1.3 (fluoranthene) and 6.0±1.8 (pyrene) mg L-1 obtained using the bacterial consortium. When the media was supplemented with 100 mg L-1 phenanthrene, after 8 days of degradation by bacterial consortium residual chrysene, fluoranthene and pyrene was 0.45±0.25, 0.02±0.02 and 0.20±0.14 mg L-1, respectively while phenanthrene was undetectable. No statistical significant (p < 0.05) difference was obtained between degradation by bacterial consortium and consortium via co-metabolism with phenanthrene rather they had a strong correlation of r = 0.99. The results suggest that bacterial consortium may be useful for the decontamination of sites polluted with high molecular weight polycyclic aromatic hydrocarbons due to synergistic effect.
  M.N. Igwo-Ezikpe , O.G. Gbenle , M.O. Ilori , J. Okpuzor and A.A. Osuntoki
  Alcaligenes faecalis was evaluated for its potential to degrade varying concentrations of chrysene and diesel oil with concomitant biosurfactant production. Biodegradation was set up for 7 days utilizing the substrates as sole carbon and energy sources. Residual chrysene obtained after degradation of 30, 50 and 100 mg L-1, respectively was 17.4±1.5, 27.2±1.2 and 28.7±1.4 mg L-1 while total petroleum hydrocarbon remaining after degradation of 3, 5, 15 and 30% (v/v) diesel oil respectively was 2.58±0.5, 3.09±1.2, 21.65±5.4 and 63.92±8.1%. Microbial cells of A. faecalis and sterilized cell-free extract from diesel oil media showed emulsifying activities against kerosene, diesel oil, engine oil, hexadecane, dodecane, xylene and hexane whereas no emulsifying activity was observed of microbial cells and sterilized cell-free extract from chrysene media. Alcaligenes faecalis cells harvested from diesel oil media also showed haemolytic activity unlike the microbial cells from chrysene media. Growth of the isolate in chrysene and diesel oil media induced secretion of protein and carbohydrate into the media which were statistically significantly (p<0.05) different compared to controls. This study portrays the potential of Alcaligenes faecalis to degrade and grow on chrysene and diesel oil and induce extracellular protein and carbohydrate with concomitant production of biosurfactant for industrial purposes and in hydrocarbon bioremediation.
 
 
 
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