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Articles by O.B. Oloyede
Total Records ( 4 ) for O.B. Oloyede
  E.C. Egwim and O.B. Oloyede
  Alpha amylase was obtained from Acha (D. exilis) sprouted for 96 h and immobilized on palm wood chips, coconut and cotton wood fibers according to standard procedures. Palm wood chips maintained enzyme activity up to 25 h and still retained about 7x10-2 mg glucose mL min-1 of residual activity while coconut and cotton wool lost all activity by 12 h. Enzyme half-life improved by 857.0, 21.4 and 28.6 folds using palm wood chips, coconut and cotton wool fibre as carriers, respectively. Palm wood clip improved Vmax and catalytic efficiency by 177 and 163%, respectively. The results concludes that palm wood chip can be used to immobilize α-amylase from D. exilis for several cycles of reuse.
  O.B. Oloyede , A.A. Odutuga , J.B. Minari and A.A. Amballi
  Sixty day-old broiler chicks were used in a 4-weeks feed trial to determine the status of raw, roasted and fermented bambara groundnut. The raw and processed bambara groundnuts were used as the sole source of protein in a basal broiler chick starter diet. Soybean based diet served as the control. There were four (4) experimental groups each made up of five birds in three replicates, allocated to the experimental diets. Application of roasting and fermentation techniques were able to reduce the levels of some antinutrients in the bambara groundnut except for oxalate that increased significantly for the fermented sample. The results showed that the serum parameters like total protein, uric acid and creatinine levels indicated that both roasted and fermented based diet fed to the birds were unable to efficiently make available the nutrients in the diets to the birds. Serum transaminases (GOT and GPT) and serum alkaline phosphatase (ALP) showed significant (p<0.05) increase in the activity of the serum of birds fed on roasted and fermented bamabara groundnut based diet. It is therefore concluded that the quality of the protein of the raw and processed bambara groundnut based diet demonstrated inferiority relative to the soybean based diet.
  O.B. Oloyede , J.B. Minari and N.O. Muhammad
  Sixty broiler chicks (day-old) were used in a 4-week feeding experiment to assess the growth response and heamatological variables of broilers-chicks fed raw, roasted and fermented bambara groundnut as a sole source of protein. Soybean based diet served as the control. There were four (4) experimental groups, each made up of five birds in three replicates, allocated to the experimental diets. The results showed that the average final live weight, average weekly weight gain and average feed intake of birds fed fermented bambara groundnut performed better than birds fed roasted bambara groundnut but demonstrated inferiority when compared to the control. Among other organs measured the relative weight of gizzard were significantly (p<0.05) influenced by the dietary treatment. Protein quality as revealed by the haematological parameters, like Haemoglobin (Hb), Packed Cell Volume (PCV) and Red Blood Cell (RBC) levels indicated that fermented based diet fed to the birds performed better than but demonstrated inferiority when compare with the soybean based diet. However, the results showed that raw bambara groundnut based diet has a high protein quality when compared with the control diet. The use of bambara groundnut therefore in the feeding of broilers in the developing countries if properly harnessed and processed can make poultry production affordable.
  A.Y. Faremi and O.B. Oloyede
  The effects of soap and detergent industrial effluents on some enzymes in the stomach of Albino rats (Rattus norvegicus) were investigated. Four concentrations (5, 25, 50 and 100% v/v) of the effluent were used as the main sources of water for four groups of rats over thirty days while feeding the rats ad libitum with a commercial rat chow such that the method of feeding did not constitute a variable. Tap water was used as the control water. The physicochemical characteristics of the effluents and tap water were determined and compared with WHO standard. The average life weight gain was measured at every fifth days. At the end of the experiment, the rats were sacrificed and activities of alanine amino transferase (ALT), aspartate amino transferase (AST), lactate dehydrogenase (LDH) and alkaline phosphatase (ALP) were monitored in the stomach of the rats as a means of assessing the biochemical implication of the consumption of the chemical effluents. Soap and detergent industrial effluents presented high level of Biochemical Oxygen Demand (BOD), chemical oxygen demand (COD), Total Dissolved Solids (TDS) and some heavy metals like Pb, Cd and Mn. There were significant (p<0.05) difference and effluent concentration dependent increase in the activity of ALT, AST, LDH while ALP activity showed an effluent concentration dependent decrease in the stomach of the rats. The results showed that the consumption of the water contaminated with soap and detergent industrial effluents may be involved in the stomach dysfunction.
 
 
 
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