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Articles by O.A. Adaramoye
Total Records ( 3 ) for O.A. Adaramoye
  T.E. Lawal , E.A. Iyayi , B.A. Adeniyi and O.A. Adaramoye
  Enzymes were extracted from four fungi: Aspergillus niger, Trichoderma viride, Rhizopus stolonifer and Mucor mucedo. The purified enzyme extracts were used to degrade Groundnut Pod (GNP) in solid state. Undegraded GNP-, degraded GNP- and Roxazyme G2G-based diets were fed to starter and finisher broilers at the rates 70 and 100 g kg-1 of diet, respectively. There was a production of a broad spectrum of enzymes from the 4 fungi. Treatment of the GNP with the fungal enzyme extracts caused a more significant (p<0.05) reduction in the crude fibre and complex carbohydrate fractions and an increase in the crude protein, metabolizable energy and phosphorus in the GNP compared to the undegraded and Roxazyme treated GNP. The amounts of glucose, fructose, galactose and sucrose in the GNP were significantly (p<0.05) increased on biodegradation with the fungal enzyme extracts. Enzyme extracts from M. mucedo and R. stolonifer were more superior in this regard compared to extracts from the other fungi. Diets containing the degraded GNP resulted in significantly (p<0.05) reduced viscosity, better apparent nutrient digestibility and performance in broilers compared to the other diets. Results suggest the possibility of production of a multienzyme complex from some common tropical fungi. These enzyme complexes are more effective in biodegrading complex carbohydrates of by-products like GNP than Roxazyme which is specific for cereal based diets.
  T.E. Lawal , E.A. Iyayi , B.A. Adeniyi and O.A. Adaramoye
  Palm Kernel Cake (PKC) was used as a substrate to elicit the production of polysaccharidases from Aspergillus niger, Trichoderma viride, Rhizopus stolonifer and Mucor mucedo. The extracted enzymes produced were purified and used to ferment PKC in solid state at the rate of 250 ml/kg of the material for 7 days. Unbiodegraded and enzyme degraded PKC were used to formulate broiler starter and finisher diets at the rates of 70 g kg-1 and 100 g kg-1, respectively. A 6th diet was formulated in which Roxazyme G2G, a commercial enzyme was used to supplement the unbiodegraded PKC at the recommended inclusion level of 0.15 g kg-1. A total of 360 1-d-old broiler chicks were randomly allocated to the 6 treatments of 6 replicates each with each replicates having 10 birds. Cellulose and hemicellulose were significantly (p<0.05) reduced in the biodegraded PKC compared with the unbiodegraded PKC and PKC supplemented with Roxazyme G2G. The level of soluble sugars increased in a similar trend. Crude protein, phosphorus and energy increased significantly (p<0.05) in the biodegraded PKC compared to that treated with Roxazyme G2G and the unbiodegraded PKC. Apparent digestibility of nutrients was significantly improved (p<0.05) in birds that received the diets based on the biodegraded PKC than those on the unbiodegraded PKC and Roxazyme G2G supplemented diets. Feed conversion and weight gain in birds were significantly (p<0.05) higher in birds on the diets based on the biodegraded PKC compared to those on diets based on the unbiodegraded PKC and Roxazyme supplemented diets. Results of the study showed that PKC can act as a substrate for the production of a multienzyme complex from the 4 fungi. The enzyme complexes so produced were more efficacious in breaking down the cellulose and hemicellulose in it compared to Roxazyme G2G which is an enzyme product specific for cereal-based diets.
  O.A. Adaramoye
  It became evident in this study that carbon tetrachloride (CCl4), can induce renal oxidative damage. The hepatoprotective effects of vitamin E (Vit. E) and kolaviron (KV), a biflavonoid complex from the seeds of Garcinia kola are well documented. The present study was designed to investigate and compare the renal protective effects of Vit. E and KV in mice given CCl4 (1.2 g kg-1) intra-peritoneally thrice a week for two weeks. CCl4 caused a marked increase in serum and renal lipid peroxidation (LPO) by 106 and 225%, respectively. Treatment with KV at 100 and 200 mg kg-1 and Vit. E at 100 mg kg-1 significantly (p<0.05) decreased the CCl4-mediated increase in LPO. Furthermore, CCl4-intoxication decreased the levels of renal reduced glutathione (GSH), superoxide dismutase (SOD) and catalase (CAT) by 44, 56 and 43%, respectively. Treatment with KV and Vit. E significantly (p<0.05) ameliorated the GSH and SOD levels. Specifically, KV at 100 and 200 mg kg-1 increased GSH by 32 and 27% and SOD levels by 50 and 53%, respectively. Likewise, treatment with Vit. E increased GSH and SOD levels by 31 and 53%, respectively. Effects on markers of renal functions showed that CCl4-intoxication significantly (p<0.05) elevated serum urea and creatinine by 287 and 186%, respectively. While treatment with Vit. E decreased serum urea and creatinine by 60 and 55%, respectively, KV produced insignificant (p>0.05) effect on these parameters. This study found KV unable to protect against CCl4-induced renal damage but confirmed the potency of Vit. E to enhance recovery from renal oxidative damage.
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