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Articles by O. Prado
Total Records ( 2 ) for O. Prado
  E.S. Pablo , A.L.M. Sandoval , M.R. Fernandez , E. Morales , O. Prado , G. Tellez and M.T.M. Quintero
  In the present study, eighty-four Hy-Line W36 laying hens in two experiments were distributed in 7 treatments with 3 replicates of four hens each. Each treatment, hens received 3 dipping/2 min every 48 h. Residual activity was done by counting lice one month after dipping. Treated hens with no live lice were reinfested with 20 lice and repeated during three months. In experiment one, aqueous suspensions of three plant extracts were tested as dips for control of MS lice: a) Neem (Azadirachta indica) 500 ppm; b) Ruda (Ruta graveolens) 11,700 ppm; or c) Solanacea (Ardisia solanacea) 50,000 ppm; d) Negative Control (water). After the first dipping, a significant difference (p<0.05) in the number of dead lice were observed in the hens that received Neem (84.1%) or Solanacea (98.1%), however, after the second and third dipping, all treated groups showed a significant increase in the number of dead lice compared with the control. Average after the 3 dips was: Neem (93.6%); Ruda (85.2%); Solanacea (98.2%); Control (49.1%). One month later, all 3 treated groups had 0 lice compared with 38 lice in the control group. Counts of live lice at two months after first reinfestation were: Neem (0); Ruda (1); Solanacea (43); Control (51). Counts of live lice at three months after second reinfestation were: Neem (0); Ruda (15); Solanacea (NA); Control (60). In experiment two, 3 aqueous suspensions were tested: group 1) Ruda tincture 50,000 ppm; group 2) Coumaphos 1,000 ppm; or group 3) M. anisopliae 50,000 ppm. After the first dipping, a significant difference in the number of dead lice were observed in the hens that received Coumaphos (100 %), however, no significant differences were observed between treatments after the second and third dipping Counts of live lice one month later were: group 1 (2 lice); group 2 (0 lice); group 3 (38 lice). Counts of live lice at two months after first reinfestation were: group 1 (13) and group 2 (16). The results of the present study suggest that some alternative bio-control methods for lice in laying hens are effective.
  G. Tellez , G.M. Nava , J.L. Vicente , M. De Franceschi , E.J. Morales , O. Prado , J.C. Terraes and B.M. Hargis
  A trial was conducted to evaluate the influence of dietary Aspergillus Meal (AM) prebiotic on intestinal development, morphology in turkey poults. Day-old Nicholas poults (n = 100) were randomly assigned to two groups. Poults in each group were divided and placed in floor pens with 50 poults each. One group served as a control and received a ration containing no added AM prebiotic. Poults in the treatment group were provided a ration supplemented with 0.2% AM prebiotic. Each dietary regimen and water were provided ad libitum to 30 days of age. Poults were humanely killed by CO2 inhalation and specimens of duodenum and distal ileum were collected at 10, 20 and 30 days. Histology sections were cut (5 μm) and stained with haematoxylin and eosin and combinations of either of periodic acid-Schiff with alcian blue, or high iron diamine with alcian blue stains to evaluate 20 intact villi on each section. The morphometric variables analyzed included villi height, villi surface area and crypt depth. In addition, goblet cells, classified as neutral, acidic or sulpho mucin cells, respectively, were quantitative for each treatment group. At all times of evaluation, AM prebiotic significantly increased the number of acid mucin cells in the duodenum, neutral mucin cells in the ileum and sulpho mucin cells in the duodenum and ileum. Villi height and villi surface area of both duodenum and ileum were significantly increased at days 10 and 30 compared to control. The present study suggest that AM prebiotic has an impact on the mucosal architecture and goblet cells proliferation in the duodenum and ileum of neonate poults.
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