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Articles by O. Oyelakin
Total Records ( 3 ) for O. Oyelakin
  A. Onasanya , A. Basso , E. Somado , E.R. Gasore , F.E. Nwilene , I. Ingelbrecht , J. Lamo , K. Wydra , M.M. Ekperigin , M. Langa , O. Oyelakin , Y. Sere , S. Winter and R.O. Onasanya
 

Case No: 26082013

This article has been withdrawn due to technical issue.

  B.O. Akanji , J.O. Ajele , A. Onasanya and O. Oyelakin
  Genetic fingerprinting of 30 Pseudomonas aeruginosa (Pa) isolates from three types of nosocomial infection cases from two Osun State Teaching Hospitals was compared using RAPD-PCR markers. Ten out of 50 operon primers tested showed polymorphism with reproducible results among the isolates and produced 131 bands of which 74 were polymorphic with sizes ranging between 200 and 3,000 bp. Cluster analysis using the 74 polymorphic markers classified the 30 Pseudomonas aeruginosa isolates into two (PgA and PgB) genetic groups. Comparing isolates proportion in each genotype based on their site of infection, antibiotics resistance pattern and geographical location, it was revealed that the proportion of urinary tract infection isolates in PgA genotype was significantly less than those in PgB genotype (z = -1.195, p<0.05) while the proportion of septicaemia isolates in PgA genotype was significantly higher than its proportion in PgB genotype (z = 1.348, p<0.05). However the proportion of wound infection isolates of PgA and PgB genotypes were significantly the same (z = -0.278, p>0.05). The PgA genotype contained few isolates with increased virulence and resistance to new antimicrobial modules and could possibly be new emerging P. aeruginosa strains from PgB genotype population. The study has critically revealed the genetic diversity and distribution among P. aeruginosa isolates in Osun State.
  A. Onasanya , P. Kiepe , A. Basso , G. Nkima , F.E. Nwilene , I. Ingelbrecht , J. Lamo , M.M. Ekperigin , R.O. Onasanya , O. Oyelakin , S. Winter and Y. Sere
  Genomic DNA fingerprinting is a useful tool for effective and reliable identification and differentiation of Xanthomonas oryzae pv. oryzae (Xoo) pathogen from rice. The study aimed to conduct molecular characterization and DNA fingerprinting of 23 Xoo isolates from East Africa and two Xoo isolates from IRRI (Philippines) as control. PCR analysis was carryout on genomic DNA of 25 Xoo isolates using 6 Xoo specific primer pairs. Cluster analyses of genetic data obtained from 25 Xoo DNA fingerprints revealed two major genotypes (GrpA and GrpB) among the 25 Xoo isolates. GrpA has three subgroups (GrpA1; GrpA2; GrpA3) and GrpB (GrpB1; GrpB2; GrpB3). GrpA genotype consists of 20 Xoo isolates from Uganda, Rwanda and Philippines while GrpB genotype has 5 Xoo isolates from Rwanda. Some Xoo isolates were identical (PX-1, PX-2; UX621, RX2101; RX554, UX623, RX4113; UX211, UX213, UX214, RX4112, UX215). The emergence of subgroup genotypes could possibly be due to mutations and interactions among isolates and strains in host cells. Some Xoo isolates from Rwanda and Uganda were identical suggesting possible pathogen migration between these countries and long-term survival. Durable resistance rice cultivars would need to overcome both GrpA and GrpB Xoo genotypes in order to survive after their deployment into different rice ecologies in East Africa.
 
 
 
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