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Articles by Novra Arya Sandi
Total Records ( 2 ) for Novra Arya Sandi
  Mitra Slipranata , Siti Isrina Oktavia Salasiaa , Widi Nugroho , Novra Arya Sandi , Colin Cargill and Sukendra Mahalaya
  Background and Objective: Streptococcus suis (S. suis) is one of the infectious agents that can cause a variety of symptoms of the disease in pigs. The Papua people live closely with pigs and the pigs raise extensively around their Honai (traditional home). The pigs are part of their way of life and part of their family. Pigs also play an important role in traditional ceremonies. Most of these laboratories would probably misidentify an isolate of S. suis. The aimed of this study was to identify of S. suis isolated from pigs in Papua. Methodology: This study was designed to isolate, identify and characterize of S. suis isolated from pig in various districts in Papua. The presence of 16s rRNA, glutamate dehydrogenase (gdh gene), muramidase released protein (mrp gene), capsular polysaccharide (cps gene) were performed by the Polymerase Chain Reaction (PCR) assay. Samples were swabbed from the tonsil of pigs and cultured an aerobically in sheep blood agar, Gram staining, biochemical tests and the growth properties in liquid media as weel as Serum Soft Agar (SSA). Results: According to results, it have been identified that 54 isolates were positive of S. suis (100%), but only 30% of isolates fermented trehalose and 74% of salicin. Most of S. suis isolates showed α-haemolytic of 88.9% on sheep blood agar and only 11.1% formed β-haemolytic. About 40.7% isolates grew with clear supernatant and 59.3% isolates with turbid supernatant. In the serum soft agar, more then half isolates grew diffuse and less than 37% isolates with compact characteristic. Based on genotype characteristics, all isolates were positive for 16s rRNA and gdh gene, no more 28% isolates were positive for mrp and cps2j gene. Conclusion: This study indicated that all isolates had been correctly identified by the biochemical methods and that PCR was more definitive and revealed the inherent problem associated with the interpretation of biochemical results.
  Novra Arya Sandi and Siti Isrina Oktavia Salasia
  Honey is natural food that common used directly by the society. Forest honey produced by wild bees (Apis dorsata) while the other type of honey produced by Apis mellifera. Beekeeping A. mellifera with monoflora or multiflora nectar is generally carried out by various countries including Indonesia. Scientifically, honey containing bioactive compounds with antimicrobial properties but still uncertain which compounds that play a role in these activities. Reported bioactive compounds that have antibacterial activity of honey are inhibine and non-inhibine. Inhibine is forming enzyme and accumulation of hydrogen peroxide (H2O2) to dilute honey and nectar. The H2O2 has also long been known as an effective antibacterial and major component of penicillin, especially notatin. The types of antibacterial compounds influenced by nectar-source plants with compounds including alkaloids, flavonoids and glycosides. Some researchers reported that beside phytochemicals, antibacterial activity due to presence of Lactic Acid Bacteria (LAB) that produce bioactive compounds with antibiotic-like activity. Some studies reported 3 LAB strains potential to produce bioactive compounds with activity resembles antibiotics, namely L. kunkeei Fhon2, L. kunkeei Lahm and L. kunkeei Yubipro with the greatest inhibition zones than other Lactobacillus. Honeybees are isolated from the stomach LAB capable of producing organic acids, free fatty acids, ethanol, benzoic acid, enzymes, H2O2 and antimicrobial peptides. Different character of the bioactive compounds will jointly deliver results on inhibition zone and broad spectrum for various types of microbial pathogens. Microbial pathogens tested including Serratia marcescens, Klebsiella aerogenes, Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus and methicilin resistant Staphylococcus aureus (MRSA).
 
 
 
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