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Articles by Nisa Rachmania Mubarik
Total Records ( 8 ) for Nisa Rachmania Mubarik
  Nisa Rachmania Mubarik , Irni Mahagiani , Amaryllis Anindyaputri , Sugeng Santoso and Iman Rusmana
  Problem statement: Chitin, a common constituent of insect exoskeleton, could be hydrolyzed by chitinase. The research was conducted to screen chitinolytic rhizobacteria isolated from rhizosphere of chilli pepper and to determine their chitinase activity in degrading chitin of whitefly, Bemisia tabaci Genn. (Hemiptera: Aleyrodidae). Whitefly is recognized as an important pest on many crops including chilli pepper. Approach: Screening and molecular identification based on 16S rRNA sequence of chitinolytic isolates, chitinase productions, measurement of chitinase activity, characterization of chitinase and effect of the chitinase treatment on whitefly were studied. Results: A total of 25 isolates of rhizobacteria formed a clear zone on solid chitin media. Two isolates, i.e., I.5 and I.21 isolates had the highest chitinolytic index. Based on sequence of 16S rRNA gene, the isolates of I.5 and I.21 were identified as Bacillus sp. and Bacillus cereus, respectively. The highest chitinolytic index and specific activity of I.5 isolate was 0.94 and 0.11 U mg-1 proteins, respectively. Maximum production of I.5 chitinase was occured after 36 h cultivation at 30°C and pH 7.0. The highest chitinolytic index and specific activity of I.21isolate was 0.75 and 0.114 U mg-1 proteins, respectively. Maximum production of I.21 chitinase was occured after 36 h cultivation at 55°C and pH 7.0. Cell culture and crude enzyme of the isolates were tested on chitin of B. tabaci and the effect was observed using a microscope and sterile water was used as a negative control. Hydrolytic observation showed that crude enzyme of I.21 isolate could degrade chitin of B. tabaci exoskeleton and the activity was better than that of I.5 isolate. Conclusion: Chitinase produced by Bacillus cereus I.21 strain has potential application as biocontrol agents for B. tabaci.
  Yadi Suryadi , Dwi Ningsih Susilowati , Triny Suryani Kadir , Zuhay Ratuz Zaffan , Nurul Hikmawati and Nisa Rachmania Mubarik
  Among diseases affecting rice production, blast, sheath and bacterial leaf blight causes significant losses. This study was aimed to control multiple rice diseases using beneficial bacterial consortium as an alternative biocontrol strategies. In vitro, greenhouses and field test were carried out to study antagonistic effect of bacterial consortium against rice pathogens. The bacterial isolates used in single or in mixture combination were apparent to reduce sheath blight, neck blast and bacterial leaf blight under in vitro test. The suspension formulation mixture of A6 (B. firmus E 65, P. aeruginosa C32b, B. cereus II 14), A8 (B. firmus E65, Serratia marcescens E31, P. aeruginosa C32b, B. cereus II 14), talc based-A8 and animal compost granule-A8 had percentage inhibition to P. oryzae of 71.04, 79.19, 63.87 and 66.68%, respectively. P. aeruginosa C32a produced glucanolytic index of 2.26 and specific activity of 0.448 U mg-1. In greenhouse test, the lowest intensity of neck blast was shown by A6 treatment. Bentonite formulation showed good effect in suppressing bacterial leaf blight lesion length in greenhouse test. The cell viability decrease was ranged from 2.39 to 18.30% among different bioformulations. Talc-A8 based formulation was stable at period of storage showing no viability lost. Talc-A5 (Bacillus firmus E65, Pseudomonas aeruginosa C32b) formulation was effective against sheath and bacterial leaf blight but showed lower effect on neck blast disease in the field. Further studies should focus on the evaluation of different carriers other than talc-based powder as stable and cheaper media, then scale-up possibilities for further applications.
  Muhammad Asril , Nisa Rachmania Mubarik and Aris Tri Wahyudi
  Chitin is a major component of fungi cell wall, mycelia, stalks and spore which can be hydrolyzed by chitinase. This study was conducted to measure the ability of chitinase producing bacteria in degrading chitin of fungal pathogens such as Curvularia affinis and Colletotrichum gloeosporioides. These pathogens caused antrachnose, leaf blight and rotting on oil palm leaves. Chitinase producing bacteria, Bacillus thuringiensis SAHA 12.08 isolate were used in this study. SAHA 12.08 showed maximum chitinase with specific activity (7.896 U mg-1 protein) at 60 h incubation. Maximum temperature and pH of chitinase activity were 35°C and 7.0, respectively. Chitinase was partially purified by 30% ammonium sulphate precipitation could increase 2.35 fold than the specific activity. The activity of partially purified chitinase was optimal at 45°C and 7.0, respectively. This chitinase was stable at optimum temperature for 180 min incubation. On SDS-PAGE analysis, the enzyme had molecular weight of 107, 102, 82, 63, 55, 46 and 44 kDa from zymogram analysis only 82 kDa protein band showed chitinase activity. In vitro and detached leaf bioassay showed that chitinase of SAHA 12.08 had antagonist activity and biocontrol efficacy to C. affinis and C. gloeosporioides in oil palm leaves.
  Eliya Mursyida , Nisa Rachmania Mubarik and Aris Tjahjoleksono
  Phosphorus (P) and potassium (K) are essential for the growth and development of plants. Most of phosphate and potassium are bound in the form of rocks P or K-minerals which not be directly absorbed by plants. The utilization of microbes including Phosphate Solubilizing Bacteria (PSB) and Potassium Solubilizing Bacteria (KSB) is deemed as an alternative to address the issue of P and K availability. The objective of the research was to select and identify the phosphate-potassium solubilizing bacteria and measure the quantitative estimation of solubilized P and K. Phosphate-potassium solubilizing bacteria were collected and regrown using dot method on Pikovskaya solid medium to solubilize P and on Aleksandrov solid medium to solubilize K, incubated for 7 days at room temperature. The QC3.a.1 and QC3.d.5 showed the highest solubilization index on Pikovskaya medium containing Ca3(PO4)2, while QC3.a.2 produced the highest amount of soluble phosphate. The QC3.a.2 showed the best of potassium solubilizing activity based on clear zone formation, while QC3.a.1 had the best ability in feldspar hydrolyzation. The three strains were Gram-negative bacteria. Phylogenetic analysis based on 16S rRNA gene sequence showed that isolate QC3.a.1 was closely related to Burkholderia, isolate QC3.a.2 was closely related to Serratia and isolate QC3.d.5 was closely related to Pseudomonas putida.
  Gergonius Fallo , Nisa Rachmania Mubarik and Triadiati
  Rice (Oryza sativa L.) is one of important and suitable corps in East Indonesia including North Central Timor (NCT) Regency. However, farmer NCT Regency have not yet use microbe as biofertilizer. The research aimed to isolate Indole Acetic Acid (IAA) producing by soil bacteria from paddy field in NCT Regency and to apply selected microbe in rice cultivation. Eight isolates were obtained and analyzed their IAA ability based on a colorimetric method using Salkowski reagent. The result showed that the producing eight isolates was able to synthesize IAA with the highest concentration produced by EP.01 isolate. The ability of these isolate to solubilize phosphate was measured by using Pikovskaya media. The EP.02 isolate has the highest ability to solubilize phosphate. The growth curve and IAA synthesis were created using EP.01 and EP.02 isolates as a model. Production of IAA was in line with the cells growth. Both EP.01 and EP.02 isolates were closely related to Bacillus sp. with 97.7 and 98.1% maximum identity, respectively. The application of Bacillus sp. solubilizer as biofertilizer on paddy field using randomized block design with the type of fertilizer as a single factor. Application of fertilizer used compost enriched with 50% NPK, had the best result on the number of filled grains per clump, dry weight of 1000 grains which were 30.76, 29.37 g and 6.3 t ha-1, respectively.
  Agnes Dame Sinta Uli , Nisa Rachmania Mubarik and Dedy Duryadi Solihin
  The decreasing productivity of silkworm cocoon is caused by attack of bacteria, fungi, protozoa and virus. The research was aimed to isolate of suspected pathogenic and biological control bacteria from damage and healthy pupae of golden silkworm (Cricula trifenestrata Helfer) and to do in vivo test of the bacteria toward 4th instar larvae of C. trifenestrata and mulberry silkworms, Bombyx mori Linn. The isolate UPC 60 was suspected as pathogenic bacteria due to cause the mortality of silkworms larvae. The bacteria UPC 40 and UPC 71 were selected as biological control bacteria which had capability to reduce the mortality of silkworms about 56.67 and 43.33%, respectively. Based on 16s RNA gene the bacteria UPC 40 had 97% identity with Alcaligenes faecalis, bacteria UPC 60 had 97% identity with Aeromonas dhakensis and bacteria UPC 71 had 98% identity with Pseudomonas stutzuri. The biological control bacteria showed antagonistic activity toward the pathogenic bacteria. The outcomes of the research that the biological control bacteria expected to be used to reduce pathogenic bacteria in the maintenance of silkworm so that the production and quality of silk yarn can be improved.
  Siti Nur Azizah , Nisa Rachmania Mubarik and Lisdar I. Sudirman
  The objective of this research was to screen and identify of chitinolytic bacteria which have an ability to inhibit the growth of G. boninense and also to characterize its chitinase activity on degrading the chitin of G. boninense. Screening of chitinolytic bacteria against G. boninense by using dual culture method on Potato Dextrose Agar (PDA) showed that bacterial isolates SAHA 12.07 and KAHN 15.12 had the highest percentage inhibition of 68,19 and 40, 29%, respectively. Based on 16S rRNA identifications, SAHA 12.07 and KAHN 15.12 were identified as Bacillus amyloliquefaciens and Serratia marcescens, respectively. Isolate SAHA 12.07 produced optimum chitinase at 48 h and KAHN 15.12 at 54 h of incubation in chitin medium. Crude chitinase of SAHA 12.07 and KAHN 15.12 have precipitated by acetone at 60 and 20%, respectively. Chitinase activity had active at pH 4-10 and temperature about 20-80°C. In vitro lysis of G. boninense bioassay showed that the chitinase of SAHA 12.07 and KAHN 15.12 were capable to inhibit the growth of mycelium G. boninense and could release of N-acetylglucosamine by incubation of G. boninense with partially purified enzyme.
  Miladiarsi , Nisa Rachmania Mubarik and Rahayu Widyastuti
  Background and Objective: Phosphate solubilizing bacteria and nitrogen fixation bacteria as inoculant will increase the availability of phosphate and nitrogen for plants. The aim of this study was to isolate phosphate solubilizing rhizobacteria that could fix atmospheric nitrogen and to measure its effect on chili plant growth in a greenhouse experiment. Materials and Methods: Soil samples for bacterial isolation were collected from cultivation area of chili plants. All samples were selected and characterized each ability in solubilizing phosphate and fixing nitrogen. Selected isolates were identified by using gene 16S rRNA and potential isolate was used as inoculant on chili plants. Results: Three selected isolates of KD2.10, KD2.13 and KE2.15 showed the higher value of phosphate solubilizing index and produced soluble phosphate as much as 102.83, 102.5 and 101.5 mg L–1, respectively. The nitrogenase activity was measured by acetylene reduction assay methods. Isolates KD2.10 and KE2.15 produced ethylene with the amount of 0.0614 and 0.0728 ppm h–1, respectively, whereas isolate KD2.13 could not be measured. The three isolates were categorized as Gram-negative bacteria and isolate KD2.10, KD2.13 and KE2.15 closely related to Burkholderia cepacia , Burkholderia diffusa and Enterobacter cloacae , respectively. The use of Burkholderia cepacia KD2.10 on chili plant growth could increase the plant height, number of leaves, wet and dry weight and plant root length. Conclusion: Isolate Burkholderia cepacia KD2.10 as phosphate solubilizing and nitrogen fixing bacteria was suggested as an effective inoculant in improving the growth of chili plants in a greenhouse experiment.
 
 
 
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