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Articles by Nihal Buzkan
Total Records ( 3 ) for Nihal Buzkan
  Nihal Buzkan and Andrew Walker
  Detection of Grapevine fanleaf virus (GFLV) in grapevine samples is difficult due to seasonal effect, low viral titer and uneven distribution of the viral particles within the plant and presence of phenolic compounds. To circumvent this problem in grape tissue, a nucleic acid purification (viral RNA) procedure is aimed to improve the extraction methods to remove the inhibitory components of the tissue. In this work, RNA from hybrid grapevines of five different populations (9621, 9623, 9630, 9631 and 9635) from the breeding program of the Department of Viticulture and Enology, University of California, Davis (CA) on which nematodes (Xiphinema index) carrying GFLV was extracted with silica particle suspension and phenol/chloroform methods. Extracted RNAs were submitted to cDNA synthesis and amplification procedure with primers specific to GFLV. The silica method could detect the virus infection from 43 cuttings although viral nucleic acid of the same samples from phenol/chloroform extraction were not amplified in PCR reaction The success of the silica methods was because phenolic compounds were bound to silica particles, eliminated by buffer solution with ethanol. The protocol does not require ultracentrifugation and is possible to complete in few hours which is much shorter than phenol/chloroform application. It also appears to be widely applicable to particularly difficult plant tissues.
  Nihal Buzkan , Veysel Oztekin , Mehmet Demir , N. Yesim Yalcin-Mendi and Muruvet Ilgin
  The research and experimental plot for peach and nectarine at University of Kahramanmaras Sutcu Imam (KSU) has been established in the growing period of 1998-1999. The severity and distribution of five virus diseases of peach and nectarine trees were determined during the 2004-2005 growing season. A total of 262 samples were individually collected and tested by DAS-ELISA for Prunus necrotic ringspot virus (PNRSV), Prune dwarf virus (PDV), Plum pox virus (PPV), Apple chlorotic leafspot virus (ACLSV) and Apple mosaic virus (ApMV). A total of 22.9% of ELISA-tested samples (60 out of 262) were infected by one (22.5 %) or two (0.4%) viruses. PPV was the most widespread virus (11.45%), followed by PNRSV (7.25%) and ACLSV (3.82%). ApMV and PDV were not detected in the all tested samples. These results demonstrated that we are facing to the problem of not having healthy nursery stocks for fruit trees.
  Yesim YALCIN MENDI , Muzaffer IPEK , Nihal BUZKAN , Yıldız AKA KACAR and Sebahattin CURUK
  Two different types of explant (proximal and flamingo-bill) from Emphasis seedlings, a hybrid cultivar of bottle gourd (Lagenaria siceraria (Molina) Stand.), germinated under dark and light conditions were cultured on 9 regeneration MS media containing various combinations of BA (0, 1.0, and 2.0 mg l-1) and IAA (0, 0.25, and 0.5 mg l-1). Comparison of the explant types showed that the flamingo-bill type explant had better shoot formation than did the proximal explant. The MS medium containing 1 mg l-1 of BA was optimal for shoot formation capacity when flamingo-bill type explants germinated in the dark (44%) and light (36%) were used. Histological analysis showed that explant cell division began after 3 days in regeneration medium and formation of primordium was observed in the tissues in culture between days 5 and 7. Differentiation of meristimatic structures was first observed after 9 days and development completed after 9-12 days in the culture.
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