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Articles by Nighat Aslam
Total Records ( 2 ) for Nighat Aslam
  Nighat Aslam , Farooq Latif , Sibtain Ahmed and Amer Jamil
  Trichoderma harzianum (E-58) was grown on Vogel`s medium containing glucose and xylan as carbon source. Glucose repressed the β-glucosidase production while xylan produced the enzyme in substantial amounts. Total RNA was isolated from mycelia of Trichoderma harzianum grown in medium containing xylan as carbon source. RT-PCR was performed on the isolated RNA. cDNA strand was synthesized by using oligo (dT)18 primer. Specific primers were used to amplify bgl gene from cDNA. The bgl gene was ligated at SmaI site of pUC18 and transformed into E. coli.
  Sibtain Ahmed , Qurrat-ul-Ain , Nighat Aslam , Saima Naeem , Sajjad-ur-Rahman and Amer Jamil
  Xylanases and cellulases are industrially important enzymes obtained from various microorganisms. Trichoderma harzianum is a preferred microorganism for the production of complete xylanases and cellulases. E-58 strain of the fungus was grown in different media including Enzyme Production Medium (EPM) and Vogel`s medium with different carbon sources like xylan, glucose and CMC to get the optimal production of xylanases and cellulases. It was found that glucose repressed the synthesis of these enzymes whereas xylan and CMC produced these enzymes in substantial amounts. Maximal activity of cellulases and xylanases was marked at pH 5.5 and at temperature 28°C. The fungus was grown for 5 days at 120 rpm in orbital shaker. Maximum enzyme activity of xylanase was found to be 1.209 IU mL-1 whereas maximum activity of cellulases (exoglucanase, endoglucanase and β-glucosidase) was found to be 2.764, 14.4 and 1.1209 IU mL-1 respectively.
 
 
 
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