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Articles by Neeti Sharma
Total Records ( 1 ) for Neeti Sharma
  Neeti Sharma , Banshi Dhar Pant and Jyoti Mathur
  Background and Objective: Plants provide defense mechanism against biotic stress through various strategies. There is involvement of secondary metabolites of plants in defense against pathogens. The objective of the study was to determine the polyphenol content and phenylalanine ammonia lyase (PAL), peroxidase and polyphenol oxidase (PPOx) enzyme activities in the pathogen inoculated and control plants of Eruca sativa (E. sativa) cultivars of Rajasthan. Materials and Methods: The present study described polyphenol, PAL, peroxidase and polyphenol oxidase activity in three cultivars of E. sativa viz. RTM-2002 (susceptible), RTM-314 (susceptible) and RTM-1212 (resistant) against Fusarium oxysporum and the polyphenols were analyzed by TLC and HPLC. Comparison of mean method was used for statistical analysis of the data, which was analyzed using SPSS-16 statistical software (SPSS Inc.) at Bioinformatics Centre in Banasthali University, Rajasthan. Statistical comparison of mean values was performed using paired t-test. Results: TLC and HPLC analysis of polyphenol content showed that it was maximum in resistant cultivar RTM-1212 and minimum in susceptible cultivar RTM-314. As compared to control plants, the activities of enzymes (PAL, peroxidase and PPOx) were higher in pathogen inoculated plants with an increase till 168 h. Thus, based on phytochemical analysis in the pathogen infected plants, it was observed that RTM-1212 cultivar of E. sativa responded more actively to pathogen attack as compared to other cultivars. Conclusion: The results indicated a role of polyphenol, PAL, peroxidase and polyphenol oxidase in the defense response of E. sativa, which is an economically important oilseed crop of semi-arid regions.
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