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Articles by Neelofar Jabeen
Total Records ( 2 ) for Neelofar Jabeen
  Phalisteen Sultan , A.S. Shawl , P.W. Ramteke , Arif Jan , Nahida Chisti , Neelofar Jabeen and S. Shabir
  A protocol has been developed for in vitro shoot proliferation from callus cultures of Podophyllum hexandrum Royle. Callus initiation occurs from root segments of established in vitro grown seedlings on Gamborg`s B5 media (half strength) supplemented with 2,4-D (0.5-1.5 mg L-1) and BA (0.2-1.0 mg L-1). The rhizome of this plant contains several important lignans and most important being podophyllotoxin, main precursor for anticancer drugs teniposide and ertoposide. In addition Podophyllum hexandrum has been reported to have radioprotection properties. Ruthless collection has led to the disappearance of this important medicinal plant from many areas of Himalayas. Thus there is need for immediate conservation of this important medicinal plant through tissue culture means. In the present study shoot proliferation occurs from callus cultures cultured on basal MS medium fortified with BA and IAA either alone or in combination (0.5-5.0 mg L-1) each. Regenerated shoots were rooted with high efficiency on MS medium fortified with activated charcoal (0.5-1.0%) and NAA (0.5-2.0 mg L-1). The rooted plantlets were transferred to green house in jiffy pots containing sand, soil and vermiculite in 1:1:1 ratio.
  Neelofar Jabeen , A.S. Shawl , G.H. Dar , Arif Jan and Phalisteen Sultan
  A protocol has been developed for in vitro shoot proliferation from callus cultures of Aconitum heterophyllum Wall. Callus initiation occurs from nodal segments on MS media fortified with NAA (0.5 mg L-1) and BAP (0.25 mg L-1). Callus was transferred on Msmediasupplemented with BAP (0.25 mg L-1) for shoot proliferation. The best response for shoot proliferation was obtained on MS media+ NAA (0.25 mg L-1) + BAP (0.5 mg L-1). The well-developed micro shoots were transferred to root induction media containing MS basalmedia + IAA (1.0 mg L-1). The rooted plantlets were finally transferred to green house for hardening and field transfer.
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