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Articles by Nazlina Ibrahim
Total Records ( 4 ) for Nazlina Ibrahim
  Amal Fayyad , Nazlina Ibrahim and Wan Ahmad Yaacob
  Acute and recurrent Herpes simplex virus type 1 (HSV-1) infections remain an important problem due to the emergence of acyclovir (ACV) resistant virus that makes the search for novel antiviral substances imperative. The aim of this study was to evaluate the antiviral activity of hexane fraction from Marrubium vulgare against Herpes simplex virus type 1 and to determine the mechanism of its action. Cytotoxicity, antiviral assay, time of addition, time of removal, virucidal activity, cells pre-treatment, penetration, staining and thin sections by transmission electron microscope and quantitative PCR assays were conducted to determine the mode action of hexane fraction. Cytotoxicity was demonstrated using the MTT assay, antiviral activities were demonstrated using plaque reduction assay and analysis of data was performed by one way ANOVA and T-test. Hexane fraction was found to inhibit type 1 Herpes simplex virus (HSV-1) in a dose-dependent manner. Further studies revealed that hexane fraction inactivated HSV-1 particles directly as was verified by transmission electron microscopy. Pretreatment of cells and penetration assays did not affect the inhibition of the virus. Hexane fraction suppressed the intracellular growth of HSV-1 in post-infection in time of addition (from 2-8 h p.i.), in time of removal (from 12-48 h p.i) and inhibited HSV-1 IE, E and L gene expressions. In summary, our findings suggest that the hexane fraction can inactivate extracellular viral particles and inhibit viral biosynthesis in host cells. The results also showed that the hexane fraction from Marrubium vulgare could be used as a potential antiviral agent.
  Noor Zarina Abd Wahab and Nazlina Ibrahim
  Background and Objective: Secondary metabolites derived from Goniothalamus umbrosus were found to have a lot of beneficial bioactive effects on human health, such as antibacterial, antivirus, anti-tumor and anti-malaria. In this study, Styrylpyrone Derivative (SPD) extracted from G. umbrosus root was tested against dengue virus type 2 (DENV-2) replication. Materials and Methods: In the present study, cytotoxicity of SPD was evaluated by a cell viability assay using 3-(4,5-dimethylthiazol-2,5-diphenyl tetrazolium bromide (MTT) assay. Focus forming unit reduction assays were carried out to evaluate the antiviral activity of SPD against DENV-2. These include post-treatment, pre-treatment and virucidal assays. Results: Cytotoxicity test of SPD in Vero cells showed the concentration of 50% of cytotoxicity (CC50) was 42.5 μM (8.5 μg mL1), this indicates that SPD is non-cytotoxic. High antiviral activity was observed in post-treatment with 100% reduction of DENV-2 foci at 25 μM of SPD. In the pre-treatment assay, more than 50% foci reduction was observed using the same concentration of SPD was observed. Whereas, more than 50% foci reduction was observed at 25 μM SPD in the virucidal assay. Conclusion: The findings indicated that the SPD from G. umbrosus has good potential for a prospective nature-based antiviral drug.
  Sumaia M.M. Bakoush , Wan A. Yaacob , Jumaat H. Adam and Nazlina Ibrahim
  Rafflesia cantleyi is locally known as Bunga Pakma has been used in Malaysian folk medicine by men as an energy drink or an aphrodisiac. However, there has been no evaluation of the effect of the repeated dosing of R. cantleyi bud. The current work was undertaken to evaluate the acclaimed aphrodisiac activity of R. cantleyi bud on male rats. The 40 adult male rats were divided into 4 groups (n =10 per group/dose). Rats in group A (control) were administered with 1 mL of distilled water while those in groups B, C and D were given same volume containing 250, 500 and 1000 mg kg–1 b.wt., of R. cantleyi bud water extract, respectively for 28 days. The effect of the extract on body weight, reproductive organ weight and serum testosterone concentration were determined. Sexual behavior parameters were monitored in male rats for weekly mounting test and on day 28 by pairing with a receptive female (1:1). The results revealed that the R. cantleyi bud water extract significantly increase body weight and serum testosterone content (p<0.05). Moreover, the bud extract markedly influence the orientation behavior of treated animals which showed more attraction towards female rats. Sexual behavior observations on the animals result revealed presence of precopulatory and copulatory behaviors (chasing, sniffing and mounting) by the tested male rats. The extract at doses 250, 500, 1000 mg kg–1 b.wt. significantly increase the frequency of mount and intromission (p<0.05). In addition, the ejaculation latency was significantly prolonged. The mount and intromission latencies were reduced significantly whereas ejaculation frequency significantly increased (p<0.05). Computed percentage of mounted, intromitted, ejaculated, index of libido and copulatory efficiency were higher in treated group compared to control group. The present study demonstrates that water extract of R. cantleyi bud increases testosterone level and enhances sexual behavior in male rats. The dose taken must be cautiously monitored.
  Sumaia M.M. Bakoush , Wan A. Yaacob , Jumaat H. Adam and Nazlina Ibrahim
  Rafflesia cantleyi (Rafflesiaceae) is a parasitic flowering plant scarcely found in the Peninsular Malaysia. The bud of this plant is sought after by folk medicinal practitioner for various uses by men and women. Scientific evidences on this plant are however limited. In this study we explored the antioxidant activity, total phenolic content and the total flavonoid content of the aqueous extracts from R. cantleyi bud parts including disk, perigone tube and bract. The antioxidant activity was determined by employing three different systems including scavenging activity of 2, 2-diphenyl-1-picrylhydrazyl radicals (DPPH), hydrogen peroxide (H2O2 ) scavenging activity and Ferric Reducing Antioxidant Power (FRAP). The antioxidant activity of bract extract was higher than those of perigone tube and disk. Total phenolic and total flavonoids contents were significantly higher (p<0.05) in the bract compared to perigone tube and disk. Strong correlation between total phenolic and antioxidant properties was indicated. The result of this study showed that bract of R. cantleyi bud possesses significant free radical scavenging property with this antioxidant activity contributed by the phenolic compounds. The results supported the traditional medicinal use of the bract as energy drink which is due to its natural sources of antioxidants.
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