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Articles by Nastiti Kusumorini
Total Records ( 5 ) for Nastiti Kusumorini
  Tyas Rini Saraswati , Wasmen Manalu , Damiana Rita Ekastuti and Nastiti Kusumorini
  An experiment was conducted to study the effects of period of turmeric powder supplementation and ration quality on egg production in quails. One hundred and fifty female quails were assigned into a completely randomized design with a 2 x 5 factorial arrangement. The first factor was ration quality consisted of two levels i.e., ration with high carbohydrate (standard protein) and ration with high protein (standard carbohydrate) contents. The second factor was a period of turmeric supplementation (at a dosage of 54 mg/quail/day) consisted of 5 levels i.e. (1) Without turmeric supplementation (control) (2) 30 days at the age of 14 to 44 days (3) 30 days at the age of 45-75 days (4) 30 days at the age of 7-8 months and (5) 8.5 months at the age of 14 days to 9 months. Each experimental unit consisted of 15 quails. Parameters measured were feed and water consumptions, total number of egg production, follicle hierarchy, liver functions, oviduct weight and length and blood metabolites. The results showed that quails supplemented with turmeric for 30 days prior to sexual maturity and for 8.5 months started before sexual maturity gave the highest egg production (20 and 17%, respectively), liver function, vitellogenin secretion, follicle development and blood metabolites. Ration with high carbohydrate with standard protein content gave the best egg production, liver function and blood metabolites. It was concluded that improvement of liver functions could increase total egg production by increasing yolk precursors synthesis and their depositions in the developing follicles.
  Tyas Rini Saraswati , Wasmen Manalu , Damiana Rita Ekastuti and Nastiti Kusumorini
  This research was designed to study the profile of the hormones estriol, progesterone, in the blood during a cycle of ovulation in controlled laying hens and laying hens that treated by giving the turmeric powder. Determination of hormone profile was done on laying hens. Six laying hens aged 20 month were divided into two experimental unit. Each experimental unit consist of three laying hens i.e., P0: control (not supplemented with turmeric powder), P1: turmeric powder supplementation at a dose of 405 mg/laying hens/day for a month. Blood was drawn via jugular vein by 4 mL. Blood sampling performed every 2 h starting after hen lay eggs during a cycle of ovulation, then retrieved and analyzed the serum levels of estrogen and progesterone. The data graphed to determine the profile of the hormones estrogen, progesterone and compared between the controlled and the treated with turmeric powder. The results showed that administration of turmeric powder can shorten the cycle of ovulation about 5 h 35 min. Turmeric powder did not affect the pattern of fluctuations in the hormone progesterone. There are 4 progesterone peaks during the first cycle of ovulation both in controlled laying hens and laying hens that treated with turmeric powder. There was a change to the hormone estriol fluctuations. In controlled laying hens takes four peaks hormone estriol in an ovulatory cycle, whereas in laying hen treated with turmeric powder was 1 hormone estriol peak occurring approximately 15 h before ovulation.
  Sunarno , Wasmen Manalu , Nastiti Kusumorini and Dewi Ratih Agungpriyono
  Physiological aging and aging due to oxidative stress decrease alanine-glutamine dipeptide concentrations in plasma and hippocampus. These conditions impact the decreased availability of glutamine in intracellular and glutathione levels in the hippocampus. Decrease in hippocampal glutathione level will be followed by decreased function of hippocampal neuron. One way to improve the function of the hippocampus is to increase the availability of glutamic acid in hippocampal neuronal cells. This study was designed to obtain the concentration profile of alanine-glutamine dipeptide in plasma and hippocampus and the repair function in the aging hippocampus is known from structural repairs to the mitochondria of neurons. The experimental rats were assigned into a completely randomized design with 2 x 2 x 2 factorial arrangement and three replications. The first factor was the age of the experimental rats, consisted of two levels i.e., 12 and 24 months. The second factor was oxidative stress consisted of two levels, i.e., without or with oxidative stress. The third factor was the concentration of alanine-glutamine dipeptide administration consisted of 2 concentrations, i.e., 0 and 7%. Administration of alanine-glutamine dipeptide 7% can improve function in the aging hippocampus, both in physiological aging or aging due to oxidative stress in younger or aged rats, in normal or oxidative stress rats. This research concluded that the alanine-glutamine dipeptide 7% gave the best results in increased alanine-glutamine dipeptide plasma and hippocampus, glutathione levels, the repair response of mitochondrial structure that mediates the repair function in the aging hippocampus.
  Ronald Tarigan , Nastiti Kusumorini and Wasmen Manalu
  Obesity has been a world-wide health problem and associated with many degenerative diseases. Two approved anti-obesity compounds, sibutramine and orlistat, have various serious side effects which limit their uses. IgY anti lipase will inhibit the activity of pancreatic lipase in the gastrointestinal tract. The objective of this study was to evaluate the effectivity of IgY anti lipase obtained from yolk as pancreatic lipase inhibitor for prevention of obesity. IgY anti lipase was obtained from the yolk of hens immunized with porcine pancreatic lipase and was purified with sodium sulphate precipitation. The protein was analyzed by SDS-PAGE and was identified by Immunoblotting. The ability of IgY anti lipase to inhibit pancreatic lipase was tested by ELISA colorimetric microplate assay at five grades of concentration. Yolk containing IgY anti lipase was then tested in rabbit to evaluate its ability to inhibit fat absorption in vivo. The result showed that IgY contained two proteins with molecular weights of 61.2 and 26.9 kDa and was positively reacted with the rabbit anti chicken coupled with HRP. IgY anti lipase and orlistat did not differ in inhibition capacity at various concentrations (p>0.05). The inhibition capacity of IgY obtained from unimmunized hen was significantly lower than IgY anti lipase and orlistat (p<0.05). Yolk containing IgY anti lipase also had the ability to inhibit dietary fat absorption in vivo, but its inhibition capacity was lower than orlistat (p<0.05). It is concluded that IgY anti lipase could be used to inhibit fat absorption to prevent obesity.
  Ronald Tarigan , Nastiti Kusumorini and Wasmen Manalu
  Obesity has been a world-wide health problem and associated with many degenerative diseases. Two approved anti-obesity compounds, sibutramine and orlistat, have various serious side effects which limit their uses. IgY anti lipase will inhibit the activity of pancreatic lipase in the gastrointestinal tract. The objective of this study was to evaluate the effectivity of IgY anti lipase obtained from yolk as pancreatic lipase inhibitor for prevention of obesity. IgY anti lipase was obtained from the yolk of hens immunized with porcine pancreatic lipase and was purified with sodium sulphate precipitation. The protein was analyzed by SDS-PAGE and was identified by Immunoblotting. The ability of IgY anti lipase to inhibit pancreatic lipase was tested by ELISA colorimetric microplate assay at five grades of concentration. Yolk containing IgY anti lipase was then tested in rabbit to evaluate its ability to inhibit fat absorption in vivo. The result showed that IgY contained two proteins with molecular weights of 61.2 and 26.9 kDa and was positively reacted with the rabbit anti chicken coupled with HRP. IgY anti lipase and orlistat did not differ in inhibition capacity at various concentrations (p>0.05). The inhibition capacity of IgY obtained from unimmunized hen was significantly lower than IgY anti lipase and orlistat (p<0.05). Yolk containing IgY anti lipase also had the ability to inhibit dietary fat absorption in vivo, but its inhibition capacity was lower than orlistat (p<0.05). It is concluded that IgY anti lipase could be used to inhibit fat absorption to prevent obesity.
 
 
 
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