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Articles by Nasser Mahdavi Shahri
Total Records ( 2 ) for Nasser Mahdavi Shahri
  Faezeh Yazdani Moghaddam , Jamshid Darvish , Nasser Mahdavi Shahri , A.S. Abdulamir and Siti Khalija Daud
  Problem statement: The digestive tract of animals including rodents has taxonomic and ecologic importance due to its ability in adaptation for different environments. Whereas colon absorbs water and carbohydrates, it has a vital role ecologically and physiologically and enjoys different histological structures in different rodents with various diets. Approach: Different rodent specimens belong to the family Muridae, namely Gerbilinae subfamily (Jerbillus nanus, Meriones Persicus, Meriones lybicus) Microtinae (Microtus, transcaspicus, Ellobius fascocapillus, Microtus sp.) Dipodidae (Alactage elater, Jucullus blanfordi) and scuridae (Funambulus penantii, spermophillus folvus), were studied via histochemistry techniques. Various parts of the digestive tract were removed and measured. Proximal colon was cut into 2-3 cm segments and fixed by bouin solution. The segments stained by lectin (PNA, WGA) chemochemistry. Results: Microscopic observations indicated that there were remarkable distinctions in the carbohydrates and glycoproteins content of the different layers of colon among different species of rodents. Both PNA and WGA lectin histochemistry showed high and significant discriminatory power among the studied species of rodents especially lectin staining in mucosal and absorptive cells (p<0.05). Goblet and epithelial cells showed much lower affinity to lectins. Conclusion: It was concluded that use of lectin histochemistry was a valid method for phylogenic characterization of rodents, may be other animals, depending on colon tissues. Moreover, Colon tissues proved to be highly distinct and variable among species of animals. This assay can also be used in determining the diet nature, geographical variation, diseases affection on different species of animals.
  Nasser Mahdavi Shahri , Fatemeh Naseri , Masoumeh Kheirabadi , Sakineh Babaie , Fereshteh Sadeghie Shakib and Mahnaz Azarniya
  The aim of this study is to introduce an experimental model to produce blastema tissue and to prepare samples for distinction studies with Transmission Electron Microscope (TEM). Blastema tissue is a group of undifferentiated cells that are able to divide and differentiate in some parts of the body. New Zealand White male rabbits with body weight of less than 2.5 kg and age of nearly 6 months were used in this study. At first with the help of punching techniques 4.5 mL holes were produced in rabbits` pinnas and then in 4, 5, 6, 7, 11, 21 and 24 days after regeneration the tissues around the punching holes were biopsied, using a grasp with more diameter than the one which the holes had been caused by and then samples were prepared for histological studies with electron and objective microscopes. Qualitative and quantitative investigations of electron micrographs demonstrated that in punching site dedifferentiation of blastema cells was obvious. The number of cells of each type were counted and they were compared with each other. The surface of organelles such as cytoplasm, nucleus, endoplasmic reticulum, golgi sacs, mitochondrions, lysosoms and light and dark vacuoles were also measured and compared and then the related data were studied statistically. According to the results obtained from morphologic (cytological) and quantitative studies (comparison between different cellular organelles), The development of Blastema tissue cells regarding the time of study is obvious, so that the existence of chondroblast cells in chondrogenesis and endothelial cells in angiogenesis in 11 and 24 days after regeneration can be seen. So, considering the importance of understanding the blastema cells ultrastructure in mammals, morphology of their development and the fact that it has not been reported so far, this study is the first step and can be continued by other researchers.
 
 
 
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