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Articles by Narsimha Reddy
Total Records ( 3 ) for Narsimha Reddy
  Lin Zhang , Cheang Sao Khoo , Sundar Rao Koyyalamudi , Nuria de Pedro and Narsimha Reddy
  Background and Objective: Medicinal herbs offer an important traditional way to prevent and cure several diseases such as cardiovascular disease, chronic inflammation and cancer as they contain bioactive compounds including those with antioxidant, immunomodulatory and anticancer activities. The purpose of this study was to determine biological activities of organics from hot water extracts of medicinal herbs and to obtain the correlation of activities with polyphenol contents. Materials and Methods: In this study, 16 herbs were selected based on their traditional medicinal uses and obtained their hot water extracts. Ethanol soluble organic molecules were separated from these extracts and their antioxidant, immunomodulatory and anticancer activities were assessed. Antioxidant activities were evaluated using DPPH, ABTS•+ scavenging methods and ferric ion reducing assay. Total phenolic and total flavonoid contents of these extracts were estimated based on the Folin-Ciocalteu and aluminium chloride colorimetric methods. The immunomodulatory properties of the herbs were determined on the basis of their ability to inhibit NO and TNF-α production in LPS induced RAW 264.7 macrophages. Cell viabilities were determined using MTT assay. The anticancer activities were measured against five human cancer cell lines. All data was analysed using one-way ANOVA and Duncan’s multiple range methods. Results: Organic molecules extracted from Alpinae officinarum (A. officinarum), Artemisia annua, Cynanchum paniculatum, Lobelia chinensis (L. chinensis), Spatholobus suberectus (S. suberectus), Xanthium sibiricum and Amauroderma rugosum (A. rugosum) have exhibited significant antioxidant activities and considerably inhibited the production of NO and TNF-α. Seven herbal extracts out of sixteen herbs studied showed highly significant anticancer activity against MCF7. The extract from Rabdosia rubescens displayed significant anticancer activities against three cancer cell lines. Observed biological activities of the extracts showed good correlation with their flavonoid contents. Conclusion: Extracts from Akebia quinata, A. officinarum, Artemisia scoparia, L. chinensis, S. suberectus and A. rugosum exhibited significant biological activities with large quantities of polyphenols. These herbs are potential candidates for the isolation of novel anticancer agents.
  Lin Zhang , Cheang Soo Khoo , Sundar Rao Koyyalamudi , Nuria de Pedro and Narsimha Reddy
  Background and Objective: It is evident from the scientific literature that plant and mushroom polysaccharides possess significant antioxidant, immunomodulatory and anticancer activities. Sixteen traditional Chinese anticancer herbs were evaluated for the immunomodulatory and anticancer potential of their polysaccharides. This study also aimed to correlate the bioactivities of these polysaccharides with their monosaccharide composition and to identify the best herbs for further detailed studies. Methods: Polysaccharides were extracted from the selected traditional Chinese medicinal (TCM) herbs and their biological activities examined. The antioxidant activities were examined using DPPH scavenging, ABTS∙+ scavenging and iron chelating assays. The immunomodulatory properties of the polysaccharides were determined by evaluating their capacity to activate mouse macrophages (RAW 264.7) to produce the cytokines, namely, interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α). The anticancer activities of the polysaccharides were determined against five human cancer cell lines. Cell viabilities were evaluated by the MTT test to assess the toxicities of these polysaccharides. The total sugar content and the monosaccharide composition of the polysaccharides were determined with a view to correlate their bioactivities with chemical constituents. All data were analyzed using ANOVA and Duncan’s multiple range methods. Results: The polysaccharides isolated from Artemisia annua L., Lobelia chinensis Lour, Amauroderma rugosum (Blume and T. nees), Artemisia scoparia Waldst. and Kit, Artemisia vulgaris L., Curcuma aromatic Salisb, Rheum palmatum L. and Cyperusrotundus Blanco showed significant anticancer, immunomodulatory and antioxidant activities with low toxicity. The results suggested that the polysaccharides extracted from A. annua, L. chinensis, A. rugosum and S. suberectus have strong potential for immunotherapy and hence suitable candidates for cancer treatment. Conclusion: Polysaccharides from several herbs were found to exhibit significant antioxidant, immunostimulatory and anticancer activities. The results demonstrated that, A. annua, L. chinensis and A. rugosum and S. suberectus are highly suitable herbs for the discovery of anticancer polysaccharides.
  Lin Zhang , Narsimha Reddy , Cheang Soo Khoo , Sundar Rao Koyyalamudi and Christopher E. Jones
  Background and Objective: Lobelia chinensis Lour is an important anticancer herb used in traditional Chinese medicine. Many botanical polysaccharides are known to exhibit immunomodulatory and anticancer activities. This research aimed to analyze the L. chinensis polysaccharides (LCPs) for their biological activities relevant to their anticancer function. Materials and Methods: Water-soluble LCPs were extracted and purified using size-exclusion chromatography to obtain two dominant polysaccharides, LCP-1 and LCP-2 having molecular masses of 1899 kDa and 5.3 kDa, respectively. The antioxidant potentials of the isolated polysaccharides were evaluated by measuring radical scavenging activities against DPPH (2,2-diphenyl-1-picrylhydrazyl radical), ABTS∙+(2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid radical) and OH(hydroxyl radical). Immunostimulatory activities of LCP-1 and LCP-2 were measured using mouse macrophages. Structure of the most active fraction (LCP-2) was determined using FT-IR and NMR spectroscopic techniques. Results: Two isolated polysaccharide fractions displayed significant antioxidant activities and stimulated the production of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6), although LCP-2 is more effective. Detailed structural characterization by FT-IR and NMR was undertaken for the most active fraction (LCP-2) and confirmed that LCP-2 is(2,1)-β-fructan. Conclusion: The results suggested that the polysaccharides isolated from Lobelia chinensis Lour are potential candidates for immune-chemotherapy and suitable for the treatment of cancer.
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