Asian Science Citation Index is committed to provide an authoritative, trusted and significant information by the
coverage of the most important and influential journals
to meet the needs of the global scientific community.
In order to obtain more insights into the molecular mechanisms of action of polyene
antibiotics in yeast cells, a number of nystatin sensitive mutants of Saccharomyces
cerevisiae were isolated by ethylmethane sulfonate mutagenesis. The mutants
were characterized in terms of their sensitivity test to stresses such as high
temperature or high concentration of monovalent and divalent cations. The invertase
overlay assay method suggests that some of these mutants are defective for vacuolar
protein sorting. By tetrad analysis four of these mutants were found to possesses
single gene mutation.
