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Articles by Nantiya Chuaychot
Total Records ( 1 ) for Nantiya Chuaychot
  Thongchai Taechowisan , Nantiya Chuaychot , Srisakul Chanaphat , Asawin Wanbanjob and Yuemao Shen
  Some endophytic actinomycetes (120) were isolated from the roots of Alpinia galanga. Identification of these endophytes was based on their morphology and amino acid composition of the whole-cell extract. Most isolates were classified as Streptomyces sp. (82), with the remainder belonging to Nocardia sp. (11), Microbispora sp. (3) and Micromonospora sp. (2). Eight isolates were unclassified and 14 were lost during subculture. The strain identified as endophytic Streptomyces sp. Tc052 strongly inhibited test microorganisms. This endophyte was cultured, the agar was extracted with organic solvent and the extract was purified on a column of silica gel to give a major component, which was identified to be kaempferol, isoscutellarin, umbelliferone and cichoriin on the basis of spectroscopic data. These compounds together with the extract were tested for their antimicrobial activity against bacteria and yeast using micro-dilution methods for the determination of Minimum Inhibitory Concentrations (MIC) and Minimum Microbicidal Concentration (MMC). The MIC values obtained with the crude extract varied from 64-128 μg mL-1 against tested microorganisms. All the isolated compounds showed various activities.
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