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Articles by Nanjappa Ashwath
Total Records ( 2 ) for Nanjappa Ashwath
  Poonam Bhatia and Nanjappa Ashwath
  Cotyledonary explants of the Red Coat cultivar were exposed to light:dark cycles in regeneration medium (MS basal+15 μM zeatin) for four weeks, with the view of elucidating the role of light in direct shoot regeneration. Light has not been found essential for shoot regeneration in tomato. However, maximum shoot regeneration response (60%) could only occur in the explants exposed to 16 h light and 8 h dark. The response declined at 2 h dark (47%) or 24 h light (40%). Comparable number of shoots was produced in the 16 h light (3.3) and 24 h dark (3.2) treatments, but in 24 h light treatment the number of shoots produced per explant was less (2.7). Shoots with maximum height were observed in 24 h light (6.3 mm) followed by 16 h light (4.5 mm) and 24 h dark (3.9 mm). The shoots regenerated in the dark were not etiolated. Mimicking of light regulated processes by exogenous application of high concentration of cytokinin can be the possible reason for the non-etiolated behaviour of the dark regenerated shoots. Although light was not essential for shoot regeneration, appropriate light:dark cycles were required for proper chlorophyll development.
  Poonam Bhatia and Nanjappa Ashwath
  To optimise medium pH for in vitro shoot regeneration and shoot growth from the cotyledon explants of tomato cv. Red Coat, the explants were inoculated onto MS medium which was maintained at a wide range of pH (4.5-7.5). Percentage shoot regeneration and number of shoots produced per explant were not significantly affected by the medium pH. The proportion of explants that produced callus and callus diameter remained unaffected by the medium pH. In contrast, the shoot height was significantly affected and taller shoots were only produced at a pH range of 5.5-6.0. Lower (4.5-5) and higher (6.5-7.5) medium pH significantly reduced shoot height. The results suggest that the explants of tomato Cv. Red Coat are moderately tolerant to a wide range of medium pH, as most of the traits studied remained unaffected by the change in media pH. However, the best regeneration and growth occurred only in the pH range 5.5-6.0.
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