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Articles by Nagendra K. Singh
Total Records ( 2 ) for Nagendra K. Singh
  Dinesh Yadav and Nagendra K. Singh
  Wheat is one of the major staple food sources of humanity consumed by majority of the world’s population. It is the single largest source of protein and second only to rice in fulfilling the daily calorie need of Indian population. From nutritional point of view wheat protein is considered to be limiting in essential amino acids like lysine, tryptophan and threonine and hence there is a need for nutritional quality improvement using modern biotechnology tools. Attempts have been made to obtain high lysine mutants in maize, barley and sorghum by plant breeders though similar mutants could not be isolated in wheat possibly due to its polyploidy nature. With recent developments in recombinant DNA technology in plant molecular biology research, including plant transformation and regeneration, alternative molecular approaches can now be implemented to alter the amino-acids composition of seed proteins for improved nutritional quality. Various molecular approaches like protein sequence modification, synthetic genes, over expression of homologous genes and transfer and expression of heterologous genes can be targeted for nutritional quality improvement. Wheat seed storage proteins classified as gliadins, glutenins, globulins and albumins have been extensively studied. Triticin, a minor storage protein of wheat endosperm, accounting for only 5% of the total seed protein is considered to be nutritionally rich due to the presence of unique lysine-rich decapeptide repeat motif inserted in the hypervariable region of this gene. It belongs to the legumin super family (11-12S globulins) of storage proteins, which predominates in the seeds of legumes and some cereals such as rice and oat. There exists three possibilities for utilizing the triticin for higher lysine content namely by increasing the length of lysine-rich hypervariable region, enhancing the expression of the original/ modified triticin gene using more efficient prolamins promoter and increasing the gene copy number. Successful attempts have been made to clone full-length triticin cDNA, amplify hypervariable region from different wheat progenitors and clone strong seed storage protein promoters from wheat, oat and rice.
  Jawahar Katara , Rupesh Deshmukh , Nagendra K. Singh and Sarvjeet Kaur
  Bacillus thuringiensis is a bacterium of huge agronomic and scientific interest. The subspecies of this bacterium colonize and kill a large variety of host insects and nematodes with a high degree of specificity. In the present investigation, 32 native isolates of Bacillus thuringiensis recovered from different regions in India and 8 known Bacillus thuringiensis strains were analyzed at the molecular level using random amplified polymorphic DNA (RAPD) markers. Seven RAPD markers were used for diversity analysis. The RAPD banding pattern data was subjected to dendrogram construction using Unweighted Pair Group Method with Arithmetic Mean (UPGMA) analysis using NTSYSpc2.2 software. Eight main clusters were formed at 25% similarity level and four isolates were standalone of these clusters. Most of the isolates were found to be diverse, even though they were isolated from the same source or location. The RAPD markers were found to be effective to distinguish B. thuringiensis native isolates recovered from different sources and locations. The results of present investigation help to understand diversity of B. thuringiensis in India, which would be exploited to find new types of B. thuringiensis endotoxins.
 
 
 
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