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Articles by Nafiseh Sadat Naghavi
Total Records ( 2 ) for Nafiseh Sadat Naghavi
  Nafiseh Sadat Naghavi , Mahdieh Golgoljam and Mojgan Akbari
  Bacteriophages have been proposed as specific alternative for chemotherapy against multidrug resistant bacterial infections. The aim of the present study was the isolation and purification of bacteriophages from sewage, which were effective against multidrug resistant bacterial isolates and detection of their morphological characteristics. Sewage and clinical samples were used for the isolation of antibiotic resistant pathogenic bacteria. The bacteriophages were isolated and purified from sewage samples by overlay cultures of the isolated bacteria after centrifugation and filtration procedures. Three isolates of the most multidrug resistant Escherichia coli, Klebsiella pneumonia and Pseudomonas aeruginosa selected for bacteriophage isolation. Most of the isolates were resistant to different classes of antibiotics in the aminoglycosides, cephalosporins, macrolides, tetracyclines and β-lactam antibiotic groups. The isolated bacteriophges showed different plaque morphologies on the bacterial cultures. The three purified bacteriophages had icosahedral or hexagonal heads. Long tails observed in Escherichia coli bacteriophages, with the total length of about 170 nm for the phages particles; short tails observed in Klebsiella pneumonia phages particles, with the approximate total lengths of 150 nm; and short tails with the approximate 35 nm total particles length observed in Pseudomonas aeruginosa bacteriophages.
  Nasibeh Afsari , Nafiseh Sadat Naghavi and Alireza Nazari
  Identification of bacterial infections involved in ornamental fishes has a great importance with considering the adverse effects on the aquatic health as well as the possibility of common diseases between human and fish. In the present study pathogenic bacteria were isolated and purified from skin, gill, kidney and brain of symptomatic goldfish samples from aquaculture ponds in Iran on the enriched culture media. The isolated bacteria have been first identified according to the morphological and biochemical characteristics. Molecular detection was done based on the amplification of specific genome fragments and sequencing of the amplified nucleotides. The isolated bacteria detected as Pseudomonas mendosina and Aeromonas spp. including Aeromonas hydrophila (the most sequence similarity), Aeromonas veronii, Aeromonas sobria and Aeromonas salmonicida (partial similarity in the genome sequence). The results of present study showed that gold fishes which are among the most favorable ornamental fishes, can be carriers of human pathogens from Pseudomonas and Aeromonas genera and this fact is necessary to considered in aquaculture healthcare systems.
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