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Articles by Nabil A. Alhakamy
Total Records ( 2 ) for Nabil A. Alhakamy
  Mohammad Y. Alfaifi , SeragEldin I. Elbehairi , Ali A. Shati , Usama A. Fahmy , Nabil A. Alhakamy and Shadab Md
  Background and Objective: Role of dietary phenolic compounds in the modification of pathophysiological conditions in cancer is immense. Thus, ellagic acid (EA), a natural polyphenolic compound has been recognized for its anticancer activity in different preclinical studies. However, clinical application is limited because of its poor aqueous solubility and thereby, inadequate oral bioavailability. The present work was aimed to formulate micellar delivery, an effective nano-delivery tool for EA, using D-α-tocopheryl polyethylene glycol succinate (TPGS) by film-hydration method. Materials and Methods: Film hydration method was introduced to encapsulate EA within the TPGS micellar structure, which was then characterized and evaluated for in vitro release study. EA-TPGS micelle was further exposed to OVACR3 to determine cancer protecting potential of the formulation. Results: The delivery system (EA-TPGS micelles) consists of spherical shape of 113.2±23 nm with 0.260±0.038 PDI and drug-encapsulation efficiency of 88.67%±3.21. In vitro release profile in the phosphate-buffer saline was found to observe sustained pattern with 67.8% cumulative release within 12 h. Further, a dose dependent cytotoxicity of EA-TPGS micelles was observed on OVACR3 cells with an IC50 value of 12.36 μM. EA-TPGS significantly reduced viable cells via arrest of G1 phase of cell cycle and thereby induce apoptosis probably through inhibiting p15 and p21. Decreased fluorescence unit in ROS determination assay also reflected potential antioxidant activity of the EA-TPGS. Conclusion: These findings strengthened that use of EA-TPGS micelles could overcome the limitations in delivery of hydrophobic chemotherapeutics through oral route.
  Usama A. Fahmy , Osama A.A. Ahmed , Mohamed A. El-moselhy , Hani Z. Asfour and Nabil A. Alhakamy
  Background and Objective: Thymoquinone (TQ) has been reported for its efficacy to inhibit various cancer stages. Nanoparticles (NPs), showed great promise as drug carriers for cytotoxic agents. This study aims to investigate the ability of zein based NPs to enhance TQ cytotoxicity in MCF-7 cells. Materials and Methods: TQ was loaded on ZN NPs using the antisolvent phase separation technique. The prepared TQ ZN NPs were investigated for size, shape, in vitro release and cytotoxicity activity in MCF-7 cells. The presentation of data was performed using mean±SD. The IBM Statistical Package for Social Science (SPSS) statistics software (version 25) (SPSS Inc., Chicago, IL, USA) was utilized for carrying out statistical analysis. Results: TQ-Zein NPs revealed spherical shaped NPs with in vitro TQ sustained release for over 36 hrs and enhanced cytotoxicity activity in MCF-7 cells when compared with either pure TQ or ZN. Cell cycle analysis results showed accumulation of MCF-7 cells in G2/M and pre-G1 phases were observed in MCF-7 cells challenged with TQ ZN NPs. A significant increase in the % of cells for early and late apoptosis in addition to the total cell death as shown by cells stained with annexin V. Conclusion: Formulation of TQ in the form of ZN based NPs improved cellular permeation and apoptotic activity of TQ that leads to potentiation of its cytotoxic activities against MCF-7cells.
 
 
 
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