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Articles by Na Li
Total Records ( 7 ) for Na Li
  Lingli Zhang , Na Li and Xueling Gao
  Objective: The aim of this study was to compare the phytochemical characteristics and in vitro antioxidant activities of blueberry wines of 10 widely grown blueberry cultivars and further study the effect of bioactive composition on antioxidant activity. Methodology: The blueberry wines of 10 blueberry cultivars from China were analyzed for their chemical composition and antioxidant activity. ‘Gardenblue’ wine possessed the highest content of total phenolic and tannins and the highest antioxidant activity than any other blueberry wine. ‘Britewell’ wine was the darkest and most purple shade of red corresponding to the highest Total Anthocyanin Value (TAC). Results: Variance analysis showed significant differences (p<0.05) in phenolic compounds (Total phenolics, anthocyanins and tannins) and antioxidant activities by DPPH, FRAP and reducing power assays among 10 blueberry wines. Correlation analysis revealed that total phenolics and tannins were distinctly responsible for the antioxidant capacity (DPPH, FRAP and reducing power). Conclusion: Among 10 blueberry wines, ‘Gardenblue’ wine can be considered as fruit wines with abundant phenolic compounds and antioxidant activities.
  Yun-Zhou Yu , Na Li , Rui-Lin Wang , Heng-Qi Zhu , Shuang Wang , Wei-Yuan Yu and Zhi-Wei Sun
  A new gene encoding the Hc domain of Clostridium botulinum neurotoxin serotype F (FHc) was designed and completely synthesized with oligonucleotides. A soluble recombinant Hc of C. botulinum neurotoxin serotype F was highly expressed in Escherichia coli with this synthetic FHc gene. Subsequently, the purified FHc was used to vaccinate mice and evaluate their survival against challenge with active botulinum neurotoxin serotype F (BoNT/F). After the administration of FHc protein mixed with Freund adjuvant via the subcutaneous route, a strong protective immune response was elicited in the vaccinated mice. Mice that were given two or three vaccinations with a dosage of 1 or 10 µg of FHc were completely protected against an intraperitoneal administration of 20,000 50% lethal doses (LD50) of BoNT/F. The BoNT/F neutralization assay showed that the sera from these vaccinated mice contained high titers of protective antibodies. Furthermore, mice were vaccinated once, twice, or three times at four different dosages of FHc using Alhydrogel (Sigma) adjuvant via the intramuscular route and subsequently challenged with 20,000 LD50 of neurotoxin serotype F. A dose response was observed in both the antibody titer and the protective efficacy with increasing dosage of FHc and number of vaccinations. Mice that received one injection of 5 µg or two injections of >=0.04 µg of FHc were completely protected. These findings suggest that the recombinant FHc expressed in E. coli is efficacious in protecting mice against challenge with BoNT/F and that the recombinant FHc subunit vaccine may be useful in humans.
  Na Li , Jie Song , Liang Kong , Shao-Heng Li , Ya-Nan Jiao , Yu-Hui Yan , Ying-Jia Yao , Ya-Kun Meng , Xiao-Fei Li , Miao-Miao Tong , Nan Zhang , Kai Kang , Ting-Guo Kang and Jing-Xian Yang
  Background and Objective: Mechanical trauma injury is caused by some external force which does harm to the vasculature, tissues and neighboring neuronal cells. This injury is a serious insult to neuronal cells which may release lactate dehydrogenase as the characteristics of cell damage. The release of inflammatory cytokines in injury cells is a normal immune response but the over expression of some pro-inflammatory cytokines such as interleukin-6 and tumor necrosis factor-α are detrimental to wound recovery. Suppression of pro-inflammatory cytokines is beneficial to alleviate mechanical trauma injury-induced cell damage. The present study aims to establish the mechanical trauma injury model in vitro and investigate the protective effect of 2,3,5,4’-tetrahydroxystilbene-2-O-glucoside on this model and its mechanism. Materials and Methods: The SH-SY5Y cells were used to establish the mechanical trauma injury model in vitro by scratching out the monolayer and generating an area devoid of cells. Then, the extent of cell damage of the model was measured by lactate dehydrogenase content determination and 12 h was confirmed as the key time point to explore 2,3,5,4’-tetrahydroxystilbene-2-O-glucoside concentration given. The cell viability was measured by cell counting kit-8 to determine the optimal concentration of drug administration. The extent of cell damage was detected by immunofluorescence analysis to observe whether 2,3,5,4’-tetrahydroxystilbene-2-O-glucoside can protect the integrity of the cell structure TUNEL staining was used to detect whether it can decrease cell apoptosis. Finally, Tested the inflammatory cytokine levels (interleukin-6, interleukin-10 and tumor necrosis factor-α) by enzyme-linked immunosorbent assays, reverse transcription-polymerase chain reaction and western blotting to clarify the mechanism of cytoprotection. Data were assessed by the SPSS version 13.0. Results: The 2,3,5, 4’-tetrahydroxystilbene-2-O-glucoside increased viability of SH-SY5Y cells and the migrative ability, protected the integrity of cell structure, reduced apoptosis, decreased pro-inflammatory cytokine levels (interleukin-6 and tumor necrosis factor-α) and increased anti-inflammatory cytokine level (interleukin-10) in mechanical trauma injury-induced SH-SY5Y cell model. Conclusion: These studies demonstrate that 2,3,5, 4’-tetrahydroxystilbene-2-O-glucoside relieves the mechanical trauma injury-induced damage in SH-SY5Y cells by attenuating the levels of inflammatory responses. This might help us to further understand the pharmacological role of 2,3,5,4’-tetrahydroxystilbene-2-O-glucoside in anti-inflammation and neuroprotection in the neural cells.
  Na Li , Jia-Bo Wang , Yan-Ling Zhao , Lin Zhang , Xi-Bo Ma , Xiao-Fei Li , Jie Song , Xin Yang , Xiao-He Xiao , Jie Tian and Ting-Guo Kang
  Background: Hepatocytes damage is sometimes closely related to oxidative stress and reactive oxygen species which are the major contributors to lipopolysaccharide-induced liver injury. Emodin, the active natural product in rhubarb of hydroxyanthraquinone skeleton, has been reported of protective activity to liver tissue, whose mechanism is generally thought of antioxidation based on chemical reaction or indirect evidence. There is no visualized evidence proved the reactive oxygen species scavenging effect of emodin in vivo. Materials and Methods: The dynamic reactive oxygen species luminescent signal in mice injured by bacillus calmette guerin and lipopolysaccharide was monitored by using the optical molecular imaging approach. Results: The elevations of serum alanine aminotransferase and aspartate transaminase activities in bacillus calmette guerin/lipopolysaccharide-injured mice were reversed by emodin, indicating the protection of emodin to hepatocytes. And emodin significantly and dose-dependently attenuated the reactive oxygen species luminescent signal elicited by bacillus calmette guerin/lipopolysaccharide, indicating visually the in vivo reactive oxygen species scavenging effect of emodin. In addition, emodin significantly and dose-dependently elevated the activity of superoxide dismutase, content of reduced glutathione and total antioxidant capacity and meanwhile decreased the contents of hydrogen peroxide, lipid peroxides and malondialdehyde in livers of bacillus calmette guerin/lipopolysaccharide-injured mice. It could be attributed to the anti-oxidative effect of emodin which helps to maintain the reactive oxygen species balance in vivo. Conclusion: Emodin can protect liver against bacillus calmette guerin/lipopolysaccharide-induced injury and the mechanism includes reactive oxygen species scavenging effect and anti-lipid peroxidation at least.
  Dongmei Li , Yan Qin , Na Li and Guangxin Wang
  Importing test paper questions into the database is a key part of initializing the question bank. This thesis proposes a method based on SVM (Support Vector Machine) and shallow parsing to recognize massive test paper and automatically finish the initialization of the question bank. This approach first use SVM to build a hyperplane to separate test paper into two parts, which are the question numbers and the questions. Secondly, automata model based on the principle of shallow parsing is constructed to judge the question numbers which are recognized by SVM and revises the wrong results. Finally, the successful recognized questions are imported into the database automatically after confirming. A large number of experimental results demonstrate that this method does not need any artificial pre-processing work. It can be used to recognize the Word test paper that contains pictures, tables and formulas. The algorithm is proved to be feasible, effective and adaptable and the recognition rate can achieve 100%.
  Yu Zhang , SoHee Kwon , Teppei Yamaguchi , Fabien Cubizolles , Sophie Rousseaux , Michaela Kneissel , Chun Cao , Na Li , Hwei-Ling Cheng , Katrin Chua , David Lombard , Adam Mizeracki , Gabriele Matthias , Frederick W. Alt , Saadi Khochbin and Patrick Matthias
  Posttranslational modifications play important roles in regulating protein structure and function. Histone deacetylase 6 (HDAC6) is a mostly cytoplasmic class II HDAC, which has a unique structure with two catalytic domains and a domain binding ubiquitin with high affinity. This enzyme was recently identified as a multisubstrate protein deacetylase that can act on acetylated histone tails, α-tubulin and Hsp90. To investigate the in vivo functions of HDAC6 and the relevance of tubulin acetylation/deacetylation, we targeted the HDAC6 gene by homologous recombination in embryonic stem cells and generated knockout mice. HDAC6-deficient mice are viable and fertile and show hyperacetylated tubulin in most tissues. The highest level of expression of HDAC6 is seen in the testis, yet development and function of this organ are normal in the absence of HDAC6. Likewise, lymphoid development is normal, but the immune response is moderately affected. Furthermore, the lack of HDAC6 results in a small increase in cancellous bone mineral density, indicating that this deacetylase plays a minor role in bone biology. HDAC6-deficient mouse embryonic fibroblasts show apparently normal microtubule organization and stability and also show increased Hsp90 acetylation correlating with impaired Hsp90 function. Collectively, these data demonstrate that mice survive well without HDAC6 and that tubulin hyperacetylation is not detrimental to normal mammalian development.
  Yan Hui Zhu , Na Li and Ming-Wei Wang
  A new lignan glycoside was isolated from the n-BuOH extract of Peperomia duclouxii C. DC., and its structure was elucidated to be 2-(4”-hydroxy-3”, 5”-dimethoxybenzyl)-3-(5′-methoxy-3′, 4′-methylenedioxybenzyl)butyrolactone-4”-O-β-D-glucopyranoside (1) by spectral methods.
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