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| Articles
by
N.K. Gangwar |
Total Records (
1 ) for
N.K. Gangwar |
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A. Kumar
,
S.V. Singh
,
A.K. Srivastava
,
N.K. Gangwar
,
P.K. Singh
,
S. Gupta
,
K.K. Chaubey
,
R. Tiwari
,
S. Chakraborty
and
K. Dhama
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Johne’s Disease (JD), caused by Mycobacterium avium
subspecies paratuberculosis, is endemic in domestic animals and adversely
affects dairy industry worldwide. In the present study, efficacies of ‘Indigenous’and commercial (Gudair, Spain) vaccines were evaluated for control of JD in
experimentally challenged goats. Goats were grouped into Sham-immunized, Indigenous
and Gudair vaccine groups. Vaccinated kids were challenged at 50 and 270 Days
Post Vaccination (DPV), with 3×109 and 5×109 ‘Indian
Bison Type strain ‘S 5’, respectively and sacrificed at 150 and 450 DPV after
1st and 2nd challenge, respectively. Vaccines were evaluated
for improvements in physical condition (diarrhea, weakness, body coat color),
clinical symptoms (shedding of bacilli, mortality, morbidity), immune responses
(cell-mediated and humoral), pathology (gross and microscopic lesions) and production
status (body weights, growth rates). Vaccinated goats gained higher body weights
vis a vis sham-immunized. Mortality was higher in sham-immunized. Cell Mediated
Immunity (CMI) response increased at 30 DPV and showed down regulation from
90 DPV onwards in vaccinated goats. Significant increase in humoral immune response
was observed in vaccinated goats at 180 DPV and maintained till 450 DPV. Microscopical
examination at 180 DPV showed reduced shedding in vaccinated groups, At 200
DPV, group 1 goats showed thickening of small intestine with corrugations specifically
at ileocaecal junction, catarrhal enteritis with infiltration of mononuclear
cells and epitheloid cells. In vaccinated groups, there were focal thickening
of intestines at 450 DPV with lesions of chronic catarrhal enteritis and presence
of lymphocyte, plasma cells and macrophages cells with a few epitheloid cells.
Monitoring of MAP DNA in the blood of experimental goats of all the groups was
done by testing of blood samples by Polymerase Chain Reaction (PCR) and the
vaccinated groups of goats revealed MAP bacilli free status upto 300 DPV. Both
the vaccines provided protection after challenge I, but since indigenous vaccine
also protected goats after challenge II, was therefore superior. In conclusion,
the indigenous vaccine must be exploited for its full potential for effective
prevention and control of this economically important disease having public
health concerns. |
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