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Articles by N.D. JAN
Total Records ( 2 ) for N.D. JAN
  A.N. Mahar , N.D. JAN , Q.I. Chachar , G.S. Markhand , M. Munir and A.Q. Mahar
  Production and infectivity to cabbage butterfly, Pieris brassicae larvae and pupae was compared to four entomopathogenic nematodes such as Steinernema carpocapsae, S. feltiae, Heterorhabditis indica and H. bacteriophora. Nematodes production of all species was determined by the number infective juveniles (IJs) established in cabbage butterfly larvae and pupae using sand and filter paper bioassay. S. carpocapsae produced the maximum number in larvae and pupae at 25°C as compared to other nematodes but production of H. indica, was better at 30°C in larvae and pupae followed by H. bacteriophora, S. carpocapsae and S. feltiae. Infectivity test of larvae and pupae was also done in sand media. Infective juveniles recovered from larvae and pupae when treated with S. carpocapsae produced maximum infective juveniles at 25°C temperatures than all other isolates. H. indica produced higher number of IJs in larvae and pupae than all other nematode isolates at 30°C. This research indicates the application of nematodes with the knowledge of insect pest biology represents a possible new strategy for controlling cabbage butterfly larvae and pupae.
  A.N. Mahar , D.A. Darban , S.R. Gowen , N.G.M. Hague , N.D. Jan , M. Munir and A.Q. Mahar
  The bacterium from Pseudomonas putida from Steinernema abbasi and its metabolic secretions caused the mortality of the Galleria mellonella pupae. Experiments were conducted in sand and filter paper on time exposure, temperature, moisture, dose and time of penetration of bacterium in pupae and tested stored or dried toxic metabolites using G. mellonella pupae as a test target organism. Death of pupae was probably due to the toxic metabolites. Pseudomonas putida cells were recovered from the haemocoele when bacterial cells were applied to the G. mellonella pupae indicating that bacterial cells can enter the haemocoele in the absence of nematode vector. Penetration of bacterium was found rapidly after application on G. mellonella pupae. Pseudomonas putida or its toxic secretions can be used as a microbial control for insect control. The experimental results indicate that there is possibility of using P. putida and its toxic secretions as a biopesticide and can contribute in the development of new microbial and biological control against insect pests.
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